Overview

  • Product name

    Human HSP60 ELISA Kit
    See all HSP60 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4 2.1%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 6.7%
  • Sample type

    Tissue Extracts, Cell Lysate
  • Assay type

    Sandwich (quantitative)
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Product overview

    ab124534 HSP60 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of HSP60 protein a 60 kDa protein encoded by the Human HSPD1 gene, in cell and tissue lysates. The assay employs an HSP60 specific antibody coated onto well plate strips.

    Standards and samples are pipetted into the wells and analyte present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti-HSP60 primary detector antibody is added. After washing away unbound primary detector antibody, HRP-conjugated secondary detector antibody specific for the primary detector antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of analyte bound. The developing blue color is measured at 600 nm.

    Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.

     

    Species– Human reactive. Non-reactive with mouse and rat. Others untested.

  • Notes

    Typical working ranges:

    HepG2 cell extract: 0.39 - 200 µg/mL

    Hela cell extract: 3.125 - 200 µg/mL

    Store all components at 4°C. This kit is stable for at least 6 months from receipt. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab124534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Analysis of HSP60 staining in Hela cells by Flow: blue: 1µg/mL HSP60 antibody; red: an equal amount of an isotype control antibody.
  • Analysis of HSP60 staining in HDFn cells by ICC: HSP60 primary antibody at 1 µg/mL (green).
  • Western blot using the HSP60 detector antibody at 1µg/mL demonstrates assay specificity. HepG2 cell lysate loaded at 15µg/lane.
  • Sensitivity of HSP60 ELISA kit is used to demonstrate the quantitative differentiation among different human cell lines.
  • Detection of endogenous HSP60 levels in different cell lysates using HSP60 ELISA kit.
  • Detection of endogenous HSP60 levels in different cell lysates using Western blot. HSP60 antibody at 1µg/mL: HepG2 cell lysate (lane 2), HeLa cell lysate (lane 3), and HDFn lysate (lane 4), each were loaded at 15µg/lane.
  • Example standard curve of serially titrated Hela cell lysate in the working range of the assay.
  • Example standard curve of serially titrated HepG2 cell lysate in the working range of the assay.

Protocols

References

ab124534 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you very much for your call today and for your questions about ab124534.

I've confirmed with the lab that the kit has been tested in a spike-recovery assay using 10% goat serum but not with human serum. The average recovery ratewas ˜78%. It has not been tested with human plasma or serum due to the limitation of resource, but it may be suitable for your assay depending on the recovery requirements.

I hope that this information will be useful, but please let me know if you have any further questions or if there is anything else that we can do for you.

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