Human I-309 ELISA Kit (CCL1) (ab100536)


  • Product name
    Human I-309 ELISA Kit (CCL1)
  • Detection method
  • Sample type
    Cell culture supernatant, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 4 pg/ml
  • Range
    4.12 pg/ml - 3000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 79.9 70% - 103%
    Plasma 97.65 88% - 110%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s I-309 (CCL-1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human I-309 (CCL-1) in plasma and cell culture supernatants. (Human I-309 concentration is pretty low in normal plasma, it may not be detected in this assay).

    This assay employs an antibody specific for Human I-309 coated on a 96-well plate. Standards and samples are pipetted into the wells and I-309 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human I-309 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of I-309 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    We have not been able to detect the endogenous Human I-309 in normal serum with ab100536, only in serum spiked with Human I-309.

  • Notes

    Optimization may be required with urine samples

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    5X Assay Diluent D 1 x 15ml
    Biotinylated anti-Human I-309 (lyophilized) 2 vials
    I-309 Microplate (12 x 8 wells) 1 unit
    Recombinant Human I-309 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Cellular localization
  • Information by UniProt
  • Alternative names
    • BF534335
    • C C motif chemokine 1
    • C-C motif chemokine 1
    • Ccl1
    • CCL1_HUMAN
    • CCR8 ligand
    • Chemokine CC Motif Ligand 1
    • Inflammatory cytokine I-309
    • P500
    • RP23-446K18.2
    • SCYA1
    • SISe
    • Small inducible cytokine A1
    • Small-inducible cytokine A1
    • T lymphocyte secreted protein I-309
    • T lymphocyte-secreted protein I-309
    • TCA3
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative standard curve using ab100536



ab100536 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

CCL1-I309 human macrophages and DC secretion

Excellent Excellent 5/5 (Ease of Use)
CCL1-I309 secretion by human macrophages and DC isolated by negative selection in culture for 3 & 7 days.
Kit easy to use, the assay takes about 6hr.
good specificity as shown by CCL1 secretion in all macrophages subtypes (M0=MCSF M1=MCSF+IFNg M2a=MCSF+IL4 DEX=MCSF+Dexamethasone IL10=MCSF+IL10 TGFb=MCSF+TGFb) and DC (GMCSF+IL4) on day 3 & 7.

Abcam user community

Verified customer

Submitted Apr 09 2014

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I am sorry that this product did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to cont...

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Thank you for forwarding your customer's data. We would like to offer some tips to improve the results seen with the standard curve.For clarity, I have separated the evaluation of the data by the two experiments.

Attempt #1


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