Overview

  • Product name
    Human ICAM1 ELISA Kit (CD54)
    See all ICAM1 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 150 pg/ml
  • Range
    150 pg/ml - 20000 pg/ml
  • Recovery

    97 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97.37 89% - 107%
    Serum 97.48 88% - 106%
    Plasma 98.67 90% - 107%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s ICAM1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human soluble ICAM1 in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for Human ICAM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and ICAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human ICAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ICAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get higher sensitivity in only 90 minutes with Human ICAM1 ELISA Kit (CD54) (ab174445) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    340X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human ICAM1 (lyophilized) 2 vials
    ICAM1 Microplate (12 x 8 wells) 1 unit
    Recombinant Human ICAM1 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.
  • Cellular localization
    Membrane
  • Alternative names
    • BB2
    • CD 54
    • CD54
    • CD54 antigen
    • Cell surface glycoprotein P3.58
    • Human rhinovirus receptor
    • ICAM 1
    • Intercellular adhesion molecule 1
    • Major group rhinovirus receptor
    • MALA2
    • MGC6195
    • MyD10
    • P3.58
    • Surface antigen of activated B cells
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab100640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100640

  • Representative standard curve using ab100640

Protocols

References

This product has been referenced in:
  • Strewe C  et al. PlanHab Study: Consequences of combined normobaric hypoxia and bed rest on adenosine kinetics. Sci Rep 8:1762 (2018). Read more (PubMed: 29379127) »
  • Mascarenhas-Melo F  et al. Diabetes abrogates sex differences and aggravates cardiometabolic risk in postmenopausal women. Cardiovasc Diabetol 12:61 (2013). Read more (PubMed: 23570342) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for contacting us.

All of the kits mentioned (ab46027, ab100512, ab100640, ab108891) are suitable for use with citrate plasma.

I hope this information has been of help. If your require any further information please do not hesitate to contact us again.

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Answer

Thank you for your enquiry and your interest in our products.

The capture antibody of the ab100640 (ICAM1 Human ELISA Kit - 1 x 96) kit is a monoclonal mouse IgG1.

If you need any further assistance in the future, please do not hesitate to contact me.

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Question
Answer

Thank you for contacting us. Here is the requested information: ab46087: Normal values for TNFa in such samples are negatives or very low, so we usually recommend to assay the human serum samples neat with Standard buffer, human serum to dilute the standard curve, or at the 1:2 dilution with standard buffer diluent PBS BSA (in that case, the standard curve should be diluted in same buffer). Below are our recommended normal human serum dilutions for the other 3 kits. These recommendations should only be used as a starting point and the optimal sample dilution needs to be determined by the enduser empirically based on researched literature and knowledge of their particular sample. ab100586: 2 fold dilution ab100610: 500-2000 fold dilution ab100640: 5-200 fold dilution I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Please accept my apologies for the miscommunication in the previous email about ab100640, the ICAM1 Human ELISA Kit. Yes, you would need to do a dilution of your samples, so that they would be within the detection range of the kit. Please let me know if there is anything I can help you with.

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Answer

Thank you for contacting Abcam. I have received a reply from the lab about ab100640, the ICAM1 Human ELISA Kit. Based on testing and the reported literature, normal levels of soluble ICAM in serum look to be in the low-to-mid ng/ml levels and not low pg/ml levels. See below for a few references. Soluble ICAM-1 looks to be even higher in most diseased serum samples therefore this kit should be able to work for your samples. http://copewithcytokines.org/cope.cgi?key=Cytokine%20Concentrations%20in%20Biological%20Fluids : 237-294 ng/ml   http://www.scielo.br/pdf/anp/v59n1/v59n1a04.pdf : 276 ± 87.3 ng/ml   http://chestjournal.chestpubs.org/content/115/4/1059.full : 263 ± 98.5 ng/ml I hope the above information and references help and if there is anything else I can do, please let me know.      

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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