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Human IDH1 knockout HeLa cell pellet (ab278819)

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Western blot - Human IDH1 knockout HeLa cell pellet (ab278819)
  • Sanger Sequencing - Human IDH1 knockout HeLa cell pellet (ab278819)

Overview

  • Product name

    Human IDH1 knockout HeLa cell pellet
    See all IDH1 kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HeLa

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 4.

  • Passage number

    <20

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

Target

  • Involvement in disease

    Glioma
    Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.

  • Sequence similarities

    Belongs to the isocitrate and isopropylmalate dehydrogenases family.

  • Post-translational
    modifications

    Acetylation at Lys-374 dramatically reduces catalytic activity.

  • Cellular localization

    Cytoplasm. Peroxisome.
  • Information by UniProt

  • Alternative names

    • Cytosolic NADP isocitrate dehydrogenase
    • Cytosolic NADP-isocitrate dehydrogenase
    • Epididymis luminal protein 216
    • Epididymis secretory protein Li 26
    • HEL-216
    • HEL-S-26
    • ICDH
    • IDCD
    • IDH
    • IDH1
    • IDHC_HUMAN
    • IDP
    • IDPC
    • Isocitrate dehydrogenase (NADP(+)) 1 cytosolic
    • Isocitrate dehydrogenase [NADP] cytoplasmic
    • Isocitrate dehydrogenase 1 (NADP+) soluble
    • NADP dependent isocitrate dehydrogenase cytosolic
    • NADP dependent isocitrate dehydrogenase peroxisomal
    • NADP(+)-specific ICDH
    • Oxalosuccinate decarboxylase
    • PICD
    see all

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab278819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 46 kDa.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 46 kDa.

Images

  • Western blot - Human IDH1 knockout HeLa cell pellet (ab278819)
    Western blot - Human IDH1 knockout HeLa cell pellet (ab278819)
    Lane 1: Wild-type HeLa cell lysate (20µg)

    Lane 2: IDH1 knockout HeLa cell lysate (20µg)

    Lanes 1- 2: Merged signal (red and green). Green - ab230949 observed at 46 kDa. Red - loading control ab8245 observed at 37 kDa.

    ab230949 Anti-IDH1 antibody [EPR21002] was shown to specifically react with IDH1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264916 (knockout cell lysate ab257221) was used. Wild-type and IDH1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab230949 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
  • Sanger Sequencing - Human IDH1 knockout HeLa cell pellet (ab278819)
    Sanger Sequencing - Human IDH1 knockout HeLa cell pellet (ab278819)
    Homozygous: Insertion of the selection cassette in exon 4

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab278819? Please let us know so that we can cite the reference in this datasheet.

ab278819 has not yet been referenced specifically in any publications.

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