Human IDO ELISA Kit (ab245710)
- Datasheet
- References
- Protocols
Overview
-
Product name
Human IDO ELISA Kit
See all Indoleamine 2, 3-dioxygenase kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% cell extract 21 3% Inter-assay Sample n Mean SD CV% cell extract 3 3.6% -
Sample type
Cell culture extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
13 pg/ml -
Range
78.125 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 93 81% - 100% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
IDO in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IDO protein in human cell extracts.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
IDO (Indoleamine 2,3-dioxygenase 1) is a key enzyme in tryptophan metabolism. It converts L-tryptophan to N-formylkynurenine, thereby reducing tryptophan levels. Overexpression of IDO leads to suppression of T and NK cells, allowing tumor metastasis. It is a well-known marker of viral infection and is currently being investigated as a clinical target for cancer, infection, allergy suppression, and transplant rejection.
-
Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IDO Capture Antibody 1 x 600µl 10X Human IDO Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human IDO Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. -
Pathway
Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2. -
Sequence similarities
Belongs to the indoleamine 2,3-dioxygenase family. - Information by UniProt
-
Alternative names
- 3-dioxygenase
- I23O1_HUMAN
- IDO
see all -
Database links
- Entrez Gene: 3620 Human
- Omim: 147435 Human
- SwissProt: P14902 Human
- Unigene: 840 Human
Associated products
-
Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab245710 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
-
Other - Human IDO ELISA Kit (ab245710)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of human IDO standard curve in 1X Cell Extraction Buffer PTR.The IDO standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native IDO in IFN stimulated HeLa cell extracts based on a 20 µg/mL extract load.The concentrations of IDO were measured in duplicate and interpolated from the IDO standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IDO concentration was determined to be 9033 pg/mL. IDO was not detected in naive HeLa cells with a 20 ?g/mL extract load.
Protocols
References
ab245710 has not yet been referenced specifically in any publications.
