RecombinantSimpleStep

Human IDO ELISA Kit (ab245710)

Submit a review Submit a question References (1)
Other - Human IDO ELISA Kit (ab245710)
  • Example of human IDO standard curve in 1X Cell Extraction Buffer PTR.
  • Interpolated concentrations of native IDO in IFN stimulated HeLa cell extracts based on a 20 µg/mL extract load.
  • Sandwich ELISA - Human IDO ELISA Kit (ab245710)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 13 pg/ml
  • Range: 78.125 pg/ml - 5000 pg/ml
  • Sample type: Cell culture extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human IDO ELISA Kit
    See all Indoleamine 2, 3-dioxygenase kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    cell extract 21 3%
    Inter-assay
    Sample n Mean SD CV%
    cell extract 3 3.6%

  • Sample type

    Cell culture extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    13 pg/ml

  • Range

    78.125 pg/ml - 5000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 93 81% - 100%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human IDO ELISA Kit (ab245710) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IDO protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IDO with 13 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Associated products

Images

  • Other - Human IDO ELISA Kit (ab245710)
    Other - Human IDO ELISA Kit (ab245710)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of human IDO standard curve in 1X Cell Extraction Buffer PTR.
    Example of human IDO standard curve in 1X Cell Extraction Buffer PTR.
    The IDO standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
  • Interpolated concentrations of native IDO in IFN stimulated HeLa cell extracts based on a 20 µg/mL extract load.
    Interpolated concentrations of native IDO in IFN stimulated HeLa cell extracts based on a 20 µg/mL extract load.

    The concentrations of IDO were measured in duplicate and interpolated from the IDO standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IDO concentration was determined to be 9033 pg/mL. IDO was not detected in naive HeLa cells with a 20 ?g/mL extract load.

  • Sandwich ELISA - Human IDO ELISA Kit (ab245710)
    Sandwich ELISA - Human IDO ELISA Kit (ab245710)
    To learn more about the advantages of recombinant antibodies see here.

References (1)

Publishing research using ab245710? Please let us know so that we can cite the reference in this datasheet.

ab245710 has been referenced in 1 publication.

  • Yang N  et al. Irradiated Tumor Fibroblasts Avoid Immune Recognition and Retain Immunosuppressive Functions Over Natural Killer Cells. Front Immunol 11:602530 (2020). PubMed: 33584669

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab245710.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com