Human IDO ELISA Kit (ab245710)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 13 pg/ml
- Range: 78.125 pg/ml - 5000 pg/ml
- Sample type: Cell culture extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IDO ELISA Kit
See all Indoleamine 2, 3-dioxygenase kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% cell extract 21 3% Inter-assay Sample n Mean SD CV% cell extract 3 3.6% -
Sample type
Cell culture extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
13 pg/ml -
Range
78.125 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 93 81% - 100% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IDO ELISA Kit (ab245710) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IDO protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IDO with 13 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IDO Capture Antibody 1 x 600µl 10X Human IDO Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human IDO Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. -
Pathway
Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2. -
Sequence similarities
Belongs to the indoleamine 2,3-dioxygenase family. - Information by UniProt
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Alternative names
- 3-dioxygenase
- I23O1_HUMAN
- IDO
see all -
Database links
- Entrez Gene: 3620 Human
- Omim: 147435 Human
- SwissProt: P14902 Human
- Unigene: 840 Human
Associated products
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Alternative Versions
Images
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Other - Human IDO ELISA Kit (ab245710)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human IDO standard curve in 1X Cell Extraction Buffer PTR.The IDO standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native IDO in IFN stimulated HeLa cell extracts based on a 20 µg/mL extract load.The concentrations of IDO were measured in duplicate and interpolated from the IDO standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IDO concentration was determined to be 9033 pg/mL. IDO was not detected in naive HeLa cells with a 20 ?g/mL extract load.
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Sandwich ELISA - Human IDO ELISA Kit (ab245710)To learn more about the advantages of recombinant antibodies see here.
References (0)
ab245710 has not yet been referenced specifically in any publications.
