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    human-ido-elisa-kit-ab245710.pdf

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Signal Transduction Metabolism Amino Acids
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RecombinantSimpleStep

Human IDO ELISA Kit (ab245710)

  • Datasheet
  • SDS
  • Protocol Booklet
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Other - Human IDO ELISA Kit (ab245710)
  • Example of human IDO standard curve in 1X Cell Extraction Buffer PTR.
  • Interpolated concentrations of native IDO in IFN stimulated HeLa cell extracts based on a 20 µg/mL extract load.
  • Sandwich ELISA - Human IDO ELISA Kit (ab245710)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 13 pg/ml
  • Range: 78.125 pg/ml - 5000 pg/ml
  • Sample type: Cell culture extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Overview

  • Product name

    Human IDO ELISA Kit
    See all Indoleamine 2, 3-dioxygenase kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    cell extract 21 3%
    Inter-assay
    Sample n Mean SD CV%
    cell extract 3 3.6%
  • Sample type

    Cell culture extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    13 pg/ml
  • Range

    78.125 pg/ml - 5000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 93 81% - 100%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human IDO ELISA Kit (ab245710) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IDO protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IDO with 13 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human IDO Capture Antibody 1 x 600µl
    10X Human IDO Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human IDO Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
  • Function

    Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.
  • Pathway

    Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
  • Sequence similarities

    Belongs to the indoleamine 2,3-dioxygenase family.
  • Target information above from: UniProt accession P14902 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • 3-dioxygenase
    • I23O1_HUMAN
    • IDO
    • IDO 1
    • IDO-1
    • IDO1
    • INDO
    • indolamine 2,3 dioxygenase
    • Indole 2 3 dioxygenase
    • indoleamine 2 3 dioxygenase
    • indoleamine 2 3 dioxygenase 1
    • Indoleamine 2,3-dioxygenase 1
    • Indoleamine pyrrole 2 3 dioxygenase
    • Indoleamine-pyrrole 2
    see all
  • Database links

    • Entrez Gene: 3620 Human
    • Omim: 147435 Human
    • SwissProt: P14902 Human
    • Unigene: 840 Human

    Associated products

    • Alternative Versions

      • Human IDO Antibody Pair - BSA and Azide free (ab256651)

    Images

    • Other - Human IDO ELISA Kit (ab245710)
      Other - Human IDO ELISA Kit (ab245710)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of human IDO standard curve in 1X Cell Extraction Buffer PTR.
      Example of human IDO standard curve in 1X Cell Extraction Buffer PTR.
      The IDO standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
    • Interpolated concentrations of native IDO in IFN stimulated HeLa cell extracts based on a 20 µg/mL extract load.
      Interpolated concentrations of native IDO in IFN stimulated HeLa cell extracts based on a 20 µg/mL extract load.

      The concentrations of IDO were measured in duplicate and interpolated from the IDO standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IDO concentration was determined to be 9033 pg/mL. IDO was not detected in naive HeLa cells with a 20 ?g/mL extract load.

    • Sandwich ELISA - Human IDO ELISA Kit (ab245710)
      Sandwich ELISA - Human IDO ELISA Kit (ab245710)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab245710? Please let us know so that we can cite the reference in this datasheet.

    ab245710 has not yet been referenced specifically in any publications.

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