Overview

  • Product name

    Human IFN gamma ELISA Kit
    See all Interferon gamma kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Sample 30 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Sample 30 10%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 5 pg/ml
  • Range

    12.5 pg/ml - 400 pg/ml
  • Recovery

    107 %

  • Assay time

    2h 40m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human interferon gamma ELISA kit is designed for the quantitative measurement of IFN gamma in supernatants, buffered solutions or serum and plasma samples and other body fluids.


    A monoclonal antibody specific for IFN gamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN gamma concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IFN gamma are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IFN gamma present in the samples.


    This kit will recognize both endogenous and recombinant Human IFN gamma.


    Get higher sensitivity in only 90 minutes with Human IFN gamma ELISA Kit (ab174443) from our SimpleStep ELISA® range.


     

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-IFN gamma Red 1 x 400µl 2 x 400µl
    Control Silver 2 vials 4 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IFN gamma Microplate (12 x 8 well strips) 1 unit 2 units
    IFN gamma Standard (Lyophilized) Yellow 2 vials 4 vials
    Standard Diluent (Serum) 1 x 7ml 2 x 7ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
  • Research areas

  • Function

    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Tissue specificity

    Released primarily from activated T lymphocytes.
  • Involvement in disease

    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • Sequence similarities

    Belongs to the type II (or gamma) interferon family.
  • Post-translational
    modifications

    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • IF 1
    • IFG
    • IFI
    • IFN gamma
    • IFN immune
    • IFN, immune
    • IFN-gamma
    • IFNG
    • IFNG_HUMAN
    • Immune interferon
    • Interferon gamma
    • Type II Interferon
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Jurkat were stimulated for 48 hours with 50 ng x mL-1 of PMA (ab120297) and 1 uM Ionomycin (ab120116) and PBMCs were stimulated for 48 hours with 2 % PHA-M (LifeTechnologies). Cell free supernatants were tested, showing results after background signal was subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab46025

Protocols

References

This product has been referenced in:

  • Ren X  et al. Effects of fluconazole on the clinical outcome and immune response in fungal co-infected tuberculosis patients. Microb Pathog 117:148-152 (2018). ELISA . Read more (PubMed: 29432913) »
  • Okamgba OC  et al. Variations in the leukocyte and cytokine profiles between placental and maternal circulation in pregnancy-associated malaria. Res Rep Trop Med 9:1-8 (2018). Read more (PubMed: 30050350) »
See all 4 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Testing reactivity for Aotus nancymaae IFNg

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Aim: To test reactivty of ELISA kit with non-human primate model Aotus nancymaae (owl monkey) IFNg.
Samples used: Culture supernatant from Aotus nancymaae peripheral blood mononuclear cells stimulated 21hrs with 20ng/ml PMA and 1ug/ml ionomycin.
Supernatant was 8 x serially diulted 1/10 and run on ELISA using protocol and reagents supplied with kit.
Results: Graph shows standard curve (black) with sample OD and calculated concentration (red). Non-stimulated supernatant also run, no signal seen (data not shown).
Conclusion: This ELISA kit is suitable to detect IFNg from Aotus nancymaae.
Raw data available on request.

Jennifer Howard

Verified customer

Submitted Aug 17 2018

Question
Answer

Yes, I can confirm you can use ab59555 Extra Standard for Interferon gamma ELISA Kit with ab46025 IFN gamma Human ELISA Kit.

Read More

Answer

Gracias por contactarnos.


He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

Tenemos varios kits contra dichas citoquinas específicos para ratón, y específicos para humano. Después de estudiar la homología entre secuencias, me temo que no podemos predecir que exista reactividad cruzada entre especies. Experimentalmente tampoco tenemos datos para confirmarlo.

Siento no poder ser de mas ayuda. No dudes en contactarnos para cualquier otra consulta.

Read More

Answer

Thank you for your patience. I have checked on the bovine IFN-gamma ELISA Kit. Unfortunately, the kit currently is not available in our catalog as the quality control tests have not yet been completed.  In general, due to the low homology between the human and bovine as well as guinea pig proteins, a human IFN gamma ELISA kit might most likely not work well for these two species, very unfortunately. I also checked our IFN gamma antibodies that were tested in ELISA, but unfortunately, we do not have any that were tested in bovine or guinea pig either. I am sorry to have no better news for you at this moment. Please do not hesitate to contact us if you need any more advice or information. 

Read More

Answer

Thank you for contacting us. I can confirm that ab46087 can be used to detect TNF alpha in human plasma samples. The cost per KIT can be obtained from our Australian distributor Sapphire Bioscience. Their address is as follows. Please discuss with them for special offers; Sapphire Bioscience Pty Ltd 126 Cope Street Waterloo - NSW 2017 Australia Tel +61296982022, 1800062088 Website http://www.sapphirebioscience.com Fax +61296981022 Email sales@sapphirebioscience.com We have ab46025 and ab46048 to detect human IFNg in human plasma. Please check the datasheets for detection limits of these. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up