Human IFN gamma ELISA Kit (ab46025)
Key features and details
- Sensitivity: 5 pg/ml
- Range: 12.5 pg/ml - 400 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IFN gamma ELISA Kit
See all Interferon gamma kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Sample 30 4.9% Inter-assay Sample n Mean SD CV% Sample 30 10% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 5 pg/ml -
Range
12.5 pg/ml - 400 pg/ml -
Recovery
107 %
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Assay time
2h 40m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human interferon gamma ELISA kit is designed for the quantitative measurement of IFN gamma in supernatants, buffered solutions or serum and plasma samples and other body fluids.
A monoclonal antibody specific for IFN gamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN gamma concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IFN gamma are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IFN gamma present in the samples.
This kit will recognize both endogenous and recombinant Human IFN gamma.
Get higher sensitivity in only 90 minutes with Human IFN gamma ELISA Kit (ab174443) from our SimpleStep ELISA® range.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 2 x 96 tests 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml 1 x 7.5ml 1 x 13ml Biotinylated anti-IFN gamma Red 1 x 400µl 2 x 400µl 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml 1 x 11ml 1 x 24ml Control Silver 2 vials 4 vials 2 vials 4 vials HRP Diluent Red 1 x 12ml 1 x 23ml 1 x 12ml 1 x 23ml IFN gamma Microplate (12 x 8 well strips) 1 unit 2 units 1 unit 2 units IFN gamma Standard (Lyophilized) Yellow 2 vials 4 vials 2 vials 4 vials Standard Diluent (Serum) 1 x 7ml 2 x 7ml 1 x 7ml 2 x 7ml Stop Reagent Black 1 x 11ml 2 x 11ml 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl 2 x 5µl 4 x 5µl -
Research areas
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Function
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
Tissue specificity
Released primarily from activated T lymphocytes. -
Involvement in disease
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
Sequence similarities
Belongs to the type II (or gamma) interferon family. -
Post-translational
modificationsProteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IF 1
- IFG
- IFI
see all -
Database links
- Entrez Gene: 3458 Human
- Omim: 147570 Human
- SwissProt: P01579 Human
- Unigene: 856 Human
Associated products
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Related Products
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SimpleStep ELISA kits
Images
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Representative Standard Curve using ab46025
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab46025 has been referenced in 15 publications.
- Ye T et al. LncRNA CCAT1 is overexpressed in tuberculosis patients and predicts their survival. Immun Inflamm Dis 10:218-224 (2022). PubMed: 34847295
- Long C et al. B7-H3 as a Target for CAR-T Cell Therapy in Skull Base Chordoma. Front Oncol 11:659662 (2021). PubMed: 34868903
- Yang L et al. Long non-coding RNA GAS5 protects against Mycoplasma pneumoniae pneumonia by regulating the microRNA-222-3p/TIMP3 axis. Mol Med Rep 23:N/A (2021). PubMed: 33760178
- Ye Y et al. miR-489-3p inhibits TLR4/NF-?B signaling to prevent inflammation in psoriasis. Exp Ther Med 22:744 (2021). PubMed: 34055060
- Zhao J et al. Optimization of GPC3-specific chimeric antigen receptor structure and its effect on killing hepatocellular carcinoma cells. Bioengineered 12:3674-3683 (2021). PubMed: 34261411