Human IFN gamma High Sensitivity ELISA Kit (ab46048)
Key features and details
- Sensitivity: 0.69 pg/ml
- Range: 0.78 pg/ml - 25 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human IFN gamma High Sensitivity ELISA Kit
See all Interferon gamma kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 3.9% Inter-assay Sample n Mean SD CV% Overall 8.6% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 0.69 pg/ml -
Range
0.78 pg/ml - 25 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 108 % - % Serum 92 % - % Plasma 72 % - % -
Assay time
3h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s IFN gamma Human High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IFN gamma in Human sera, plasmas, buffered solutions and cell culture media.
A monoclonal antibody specific for IFN gamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN gamma concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IFN gamma are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IFN gamma present in the samples.
This kit will recognize both endogenous and recombinant Human IFN gamma.
Get results in 90 minutes with Human IFN gamma ELISA Kit (ab174443) from our SimpleStep ELISA® range.
-
Platform
Microplate
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 2 x 96 tests 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 15ml 200X Wash Buffer White 2 x 10ml 1 x 10ml Amplification Diluent Brown & blue spot 1 x 25ml 1 x 15ml Amplifier Yellow 2 x 200µl 1 x 200µl Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7.5ml Biotinylated anti-IFN gamma Red 2 x 0.4ml 1 x 0.4ml Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml HRP Diluent Red 2 x 23ml 1 x 23ml IFN gamma Microplate (12 x 8 well strips) 2 units 1 unit IFN gamma Standard (Lyophilized) Yellow 4 vials 2 vials Plastic Plate Covers 4 units 2 units Stop Reagent Black 2 x 11ml 1 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl -
Research areas
-
Function
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
Tissue specificity
Released primarily from activated T lymphocytes. -
Involvement in disease
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
Sequence similarities
Belongs to the type II (or gamma) interferon family. -
Post-translational
modificationsProteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- IF 1
- IFG
- IFI
see all -
Database links
- Entrez Gene: 3458 Human
- Omim: 147570 Human
- SwissProt: P01579 Human
- Unigene: 856 Human
Associated products
-
SimpleStep ELISA kits
Images
-
IFN gamma measured in cell culture supernatants from control or treated (48 hours; 2% PHA-M; LifeTechnologies) PBMCs, diluted 1/100-1/10000 (duplicates +/- SD).
-
IFN gamma measured in undiluted human biological fluids; mouse and rat samples (plasma and serum) were below limit of detection (duplicates +/- SD).
-
Dilution curves of IFN gamma (ab46048) and NIBSC standard (87/586). One ng of standard IFN gamma corresponds to 12.3 (+/- 1.3) IU NIBSC 87/586. Background signal subtracted (duplicates; +/- SD).
-
Representative Standard Curve using ab46048
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab46048 has been referenced in 3 publications.
- Xu W et al. Alterations in bile acids as metabolic signatures in the patients with human adenovirus type 7 infection. Front Med (Lausanne) 9:896409 (2022). PubMed: 36160124
- Wang X et al. Penehyclidine hydrochloride alleviates lipopolysaccharide-induced acute respiratory distress syndrome in cells via regulating autophagy-related pathway. Mol Med Rep 23:N/A (2021). PubMed: 33300058
- Weng J et al. CD44 3'-Untranslated Region Functions as a Competing Endogenous RNA to Enhance NK Sensitivity of Liver Cancer Stem Cell by Regulating ULBP2 Expression. Int J Biol Sci 15:1664-1675 (2019). PubMed: 31360109