Key features and details
- Mouse monoclonal [MHK-49] to Human Ig light chain
- Suitable for: ICC/IF, ELISA, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Human Ig light chain antibody [MHK-49]
See all Human Ig light chain primary antibodies
DescriptionMouse monoclonal [MHK-49] to Human Ig light chain
SpecificityThis antibody is specific for both soluble and membrane human immunoglobulin light chain kappa. It does not react with human immunoglobulin light chain lambda or heavy chain mu.
Tested applicationsSuitable for: ICC/IF, ELISA, Flow Cytmore details
Species reactivityReacts with: Human
Full length protein corresponding to Human Human Ig light chain.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab48536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration. This antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.|
|Flow Cyt||Use 0.5µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
RelevanceThere are only two types of light chain: kappa and lambda in mammals. Other types of light chains are found in lower vertebrates as the Ig-Light-Iota chain in Chondrichthyes and Teleostei. In each antibody, only one type is present and the two chains are identical. Each light chain has two successive domains: one constant and one variable domain. In humans 60% of light chains are kappa and 40% lambda,whereas in the mouse 95% of light chains are kappa. The amino acid sequences of lambda chains vary slightly at a few positions, allowing them to be classified into subtypes. The number of subtypes varies between species. Monoclonal immunoglobulin free light chains (FLC) are found in the serum and urine (Bence-Jones protein) of patients with a number of B-cell proliferative disorders, including multiple myeloma. Changes in serum FLC concentrations can provide a rapid and sensitive indication of response to treatment.
Cellular localizationCell Membrane: single-pass type I membrane protein and Secreted
- HCAK1 antibody
- Ig kappa chain antibody
- IGKC antibody
ab48536 has not yet been referenced specifically in any publications.