Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM129] to Human Ig light chain (Biotin)
- Suitable for: Flow Cyt, Sandwich ELISA, ICC/IF, IHC-P, ELISA
- Reacts with: Human
- Conjugation: Biotin
Product nameAnti-Human Ig light chain antibody [RM129] (Biotin)
See all Human Ig light chain primary antibodies
DescriptionRabbit monoclonal [RM129] to Human Ig light chain (Biotin)
Tested applicationsSuitable for: Flow Cyt, Sandwich ELISA, ICC/IF, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Does not react with: Mouse, Rat, Goat
Full length protein corresponding to Human IgG. Purified Native Human IgG from Human Plasma
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 48.91% PBS, 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesProtein A affinity purified from an animal origin–free culture supernatant.
Our Abpromise guarantee covers the use of ab193185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|Sandwich ELISA||Use a concentration of 0.02 - 0.25 µg/ml.
May be paired with ab195574.
|ICC/IF||Use a concentration of 0.5 - 2 µg/ml.|
|IHC-P||Use a concentration of 0.5 - 2 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
RelevanceThere are only two types of light chain: kappa and lambda in mammals. Other types of light chains are found in lower vertebrates as the Ig-Light-Iota chain in Chondrichthyes and Teleostei. In each antibody, only one type is present and the two chains are identical. Each light chain has two successive domains: one constant and one variable domain. In humans 60% of light chains are kappa and 40% lambda,whereas in the mouse 95% of light chains are kappa. The amino acid sequences of lambda chains vary slightly at a few positions, allowing them to be classified into subtypes. The number of subtypes varies between species. Monoclonal immunoglobulin free light chains (FLC) are found in the serum and urine (Bence-Jones protein) of patients with a number of B-cell proliferative disorders, including multiple myeloma. Changes in serum FLC concentrations can provide a rapid and sensitive indication of response to treatment.
Cellular localizationCell Membrane: single-pass type I membrane protein and Secreted
- HCAK1 antibody
- Ig kappa chain antibody
- IGKC antibody
A titer Sandwich ELISA using ab193185 as the detection antibody. The plate, coated with the capture antibody anti-human IgG, was loaded with different amounts of human IgG. A serial dilution of ab193185 was used as the detection antibody, followed with an alkaline phosphatase conjugated streptavidin.
ELISA of human immunoglobulins shows ab193185 reacts to both kappa and lambda light chains of human immunoglobulins. No cross reactivity with mouse, rat, or goat immunoglobulin light chain. The plate was coated with 50ng/well of different immunglobulins. 100 ng/mL or 20 ng/mL was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ab193185 has not yet been referenced specifically in any publications.