Recombinant
RabMAb

Recombinant Anti-Human IgA antibody [EPSISR10] (ab133660)

Overview

  • Product name

    Anti-Human IgA antibody [EPSISR10]
    See all Human IgA primary antibodies
  • Description

    Rabbit monoclonal [EPSISR10] to Human IgA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Human IgA aa 250-350. The exact sequence is proprietary.

  • Positive control

    • Human plasma and tonsil lysates; Human tonsil and stomach tissues; purified Human IgA.
  • General notes

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPSISR10
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 37 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance

      Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system.
    • Cellular localization

      Secreted
    • Database links

    • Alternative names

      • FLJ14473 antibody
      • FLJ35065 antibody
      • FLJ35500 antibody
      • FLJ36402 antibody
      • FLJ39698 antibody
      • FLJ40001 antibody
      • FLJ41548 antibody
      • FLJ41552 antibody
      • FLJ41789 antibody
      • FLJ43248 antibody
      • FLJ43594 antibody
      • FLJ44293 antibody
      • FLJ46028 antibody
      • FLJ46621 antibody
      • FLJ46724 antibody
      • IgA1 antibody
      • Igh2 antibody
      • IGHA1 antibody
      • IGHA2 antibody
      • Immunoglobulin heavy constant alpha 1 antibody
      • Immunoglobulin heavy constant alpha 2 (A2m marker) antibody
      • Immunoglobulin heavy constant alpha 2 antibody
      see all

    Images

    • All lanes : Anti-Human IgA antibody [EPSISR10] (ab133660) at 1/1000 dilution

      Lane 1 : Human plasma lysate
      Lane 2 : Human tonsil lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 37 kDa

    • Anti-Human IgA antibody [EPSISR10] (ab133660) at 1/1000 dilution + Purified Human IgA at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 37 kDa

    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling Human IgA with ab133660 at 1/250 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human stomach tissue labelling Human IgA with ab133660 at 1/250 dilution.

    References

    ab133660 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133660.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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