Recombinant Anti-Human IgA antibody [RM128] (ab193189)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgA antibody [RM128] (ab193189)](https://www.abcam.com/ps/products/193/ab193189/Images/ab193189-266961-anti-human-iga-antibody-rm128-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgA antibody [RM128] (ab193189)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM128] to Human IgA
- Suitable for: ELISA, IHC-P, Sandwich ELISA
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-Human IgA antibody [RM128]
See all Human IgA primary antibodies -
Description
Rabbit monoclonal [RM128] to Human IgA -
Host species
Rabbit -
Specificity
ab193189 reacts with Human IgA, including both IgA1 and IgA2. No cross reactivity with Human IgG, IgM, IgD, or IgE. -
Tested applications
Suitable for: ELISA, IHC-P, Sandwich ELISAmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Full length protein corresponding to Human IgA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM128 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use a concentration of 10 - 200 µg/ml.
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| IHC-P |
Use a concentration of 0.5 - 2 µg/ml.
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| Sandwich ELISA |
Use a concentration of 0.05 - 0.2 µg/ml.
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| Notes |
|---|
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ELISA
Use a concentration of 10 - 200 µg/ml. |
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IHC-P
Use a concentration of 0.5 - 2 µg/ml. |
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Sandwich ELISA
Use a concentration of 0.05 - 0.2 µg/ml. |
Target
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Relevance
Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 3493 Human
- Entrez Gene: 3494 Human
- Omim: 146900 Human
- Omim: 147000 Human
- SwissProt: P01876 Human
- SwissProt: P01877 Human
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Alternative names
- FLJ14473 antibody
- FLJ35065 antibody
- FLJ35500 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgA antibody [RM128] (ab193189)
Immunohistochemical analysis of human lymphoid tissue labelling IgA with ab193189 at 0.1 - 1µg/mL.
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![Sandwich ELISA - Anti-Human IgA antibody [RM128] (ab193189)](https://www.abcam.com/ps/products/193/ab193189/Images/ab193189-230345-anti-human-iga-antibody-rm128-sandwich-elisa.jpg)
Sandwich ELISA - Anti-Human IgA antibody [RM128] (ab193189)Sandwich ELISA of Human IgA using ab193189 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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![Sandwich ELISA - Anti-Human IgA antibody [RM128] (ab193189)](https://www.abcam.com/ps/products/193/ab193189/Images/ab193189-230348-anti-human-iga-antibody-rm128-sandwich-elisa.jpg)
Sandwich ELISA - Anti-Human IgA antibody [RM128] (ab193189)Sandwich ELISA of Human IgA using ab193189 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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![ELISA - Anti-Human IgA antibody [RM128] (ab193189)](https://www.abcam.com/ps/products/193/ab193189/Images/ab193189-230347-anti-human-iga-antibody-rm128-elisa.jpg)
ELISA - Anti-Human IgA antibody [RM128] (ab193189)ELISA analysis of Human immunoglobulins coated at 50 ng/well. ab193189 at 200 ng/mL, 50 ng/mL,
or 10 ng/mL was used as the primary antibody and alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. the results shows taht ab193189 reacted to both Human IgA1 & IgA2. There was no cross reactivity with Human IgG, IgM, IgD, or IgE. -
![ELISA - Anti-Human IgA antibody [RM128] (ab193189)](https://www.abcam.com/ps/products/193/ab193189/Images/ab193189-230346-anti-human-iga-antibody-rm128-elisa.jpg)
ELISA - Anti-Human IgA antibody [RM128] (ab193189)ELISA analysis of different amounts of Human IgA detected using a serial dilution of ab193189 as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.
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![Anti-Human IgA antibody [RM128] (ab193189)](https://www.abcam.com/ps/products/193/ab193189/Images/ab193189-6-benefits-of-recombinant-antibodies.png)
Anti-Human IgA antibody [RM128] (ab193189)
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab193189 has been referenced in 2 publications.
- Puligedda RD et al. Human IgA Monoclonal Antibodies That Neutralize Poliovirus, Produced by Hybridomas and Recombinant Expression. Antibodies (Basel) 9:N/A (2020). PubMed: 32121092
- Li W et al. Radiation therapy-induced reactive oxygen species specifically eliminates CD19+IgA+ B cells in nasopharyngeal carcinoma. Cancer Manag Res 11:6299-6309 (2019). PubMed: 31372036