For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    By product type
    Proteins and Peptides
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Lysates
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    • Protocols & troubleshooting
    • Technical FAQs
    • Get technical help
    • RabMAb Advantages
    • Buying FAQs
    • Antibody Guide
    • Scientific webinars
    • Biochemical product FAQ
    • Support resources
    • eProcurement
    Check out our protocols

    Visit protocols and troubleshooting or check them out using the Abcam app for iPhone

    Protocols and troubleshooting

    iPhone app

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    human-iga-antibody-rm128-ab193189.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Immunoglobulins Heavy Chain IgA
Share by email
Recombinant

Recombinant Anti-Human IgA antibody [RM128] (ab193189)

  • Datasheet
Submit a review Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgA antibody [RM128] (ab193189)
  • Sandwich ELISA - Anti-Human IgA antibody [RM128] (ab193189)
  • Sandwich ELISA - Anti-Human IgA antibody [RM128] (ab193189)
  • ELISA - Anti-Human IgA antibody [RM128] (ab193189)
  • ELISA - Anti-Human IgA antibody [RM128] (ab193189)

Add compatible products

Primary

Product image

 

Secondary

Product image

 

Unfortunately, one or more of the products below are unavailable in your country/region.

Sorry, we can't display this right now.
Please contact us to place your order, or try again later.

View more associated products

  • Datasheet
  • References (1)
  • Protocols

Overview

  • Product name

    Anti-Human IgA antibody [RM128]
    See all Human IgA primary antibodies
  • Description

    Rabbit monoclonal [RM128] to Human IgA
  • Host species

    Rabbit
  • Specificity

    ab193189 reacts with Human IgA, including both IgA1 and IgA2. No cross reactivity with Human IgG, IgM, IgD, or IgE.
  • Tested applications

    Suitable for: ELISA, Flow Cyt, IHC-P, ICC/IF, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human Human IgA.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 48% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RM128
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgA

Associated products

  • Alternative Versions

    • Anti-IgA antibody [RM128] (Biotin) (ab224184)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • Recombinant Human IgA2 protein (ab158743)
    • Native Human IgA1 protein (ab91020)

Applications

Our Abpromise guarantee covers the use of ab193189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 10 - 200 µg/ml.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P Use a concentration of 0.5 - 2 µg/ml.
ICC/IF Use a concentration of 0.5 - 2 µg/ml.
Sandwich ELISA Use a concentration of 0.05 - 0.2 µg/ml.

Target

  • Relevance

    Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system.
  • Cellular localization

    Secreted
  • Database links

    • Entrez Gene: 3493 Human
    • Entrez Gene: 3494 Human
    • Omim: 146900 Human
    • Omim: 147000 Human
    • SwissProt: P01876 Human
    • SwissProt: P01877 Human
    • Alternative names

      • FLJ14473 antibody
      • FLJ35065 antibody
      • FLJ35500 antibody
      • FLJ36402 antibody
      • FLJ39698 antibody
      • FLJ40001 antibody
      • FLJ41548 antibody
      • FLJ41552 antibody
      • FLJ41789 antibody
      • FLJ43248 antibody
      • FLJ43594 antibody
      • FLJ44293 antibody
      • FLJ46028 antibody
      • FLJ46621 antibody
      • FLJ46724 antibody
      • IgA1 antibody
      • Igh2 antibody
      • IGHA1 antibody
      • IGHA2 antibody
      • Immunoglobulin heavy constant alpha 1 antibody
      • Immunoglobulin heavy constant alpha 2 (A2m marker) antibody
      • Immunoglobulin heavy constant alpha 2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgA antibody [RM128] (ab193189)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgA antibody [RM128] (ab193189)

      Immunohistochemical analysis of human lymphoid tissue labelling IgA with ab193189 at 0.1 - 1µg/mL.

    • Sandwich ELISA - Anti-Human IgA antibody [RM128] (ab193189)
      Sandwich ELISA - Anti-Human IgA antibody [RM128] (ab193189)

      Sandwich ELISA of Human IgA using ab193189 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

    • Sandwich ELISA - Anti-Human IgA antibody [RM128] (ab193189)
      Sandwich ELISA - Anti-Human IgA antibody [RM128] (ab193189)

      Sandwich ELISA of Human IgA using ab193189 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

    • ELISA - Anti-Human IgA antibody [RM128] (ab193189)
      ELISA - Anti-Human IgA antibody [RM128] (ab193189)

      ELISA analysis of Human immunoglobulins coated at 50 ng/well. ab193189 at 200 ng/mL, 50 ng/mL,
      or 10 ng/mL was used as the primary antibody and alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. the results shows taht ab193189 reacted to both Human IgA1 & IgA2. There was no cross reactivity with Human IgG, IgM, IgD, or IgE.

    • ELISA - Anti-Human IgA antibody [RM128] (ab193189)
      ELISA - Anti-Human IgA antibody [RM128] (ab193189)

      ELISA analysis of  different amounts of Human IgA detected using a serial dilution of ab193189 as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Datasheets and documents

    • Datasheet
  • References

    This product has been referenced in:

    • Li W  et al. Radiation therapy-induced reactive oxygen species specifically eliminates CD19+IgA+ B cells in nasopharyngeal carcinoma. Cancer Manag Res 11:6299-6309 (2019). Read more (PubMed: 31372036) »
    See 1 Publication for this product

    Publishing research using ab193189? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab193189.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & advice
    • Buying FAQs
    • RabMAb products
    • Biochemical product FAQs
    Company
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2019 Abcam plc. All rights reserved.