Recombinant

Recombinant Anti-Human IgA antibody [RM128] (ab193189)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [RM128] to Human IgA
  • Suitable for: ELISA, IHC-P, Sandwich ELISA
  • Reacts with: Human

Overview

  • Product name

    Anti-Human IgA antibody [RM128]
    See all Human IgA primary antibodies

  • Description

    Rabbit monoclonal [RM128] to Human IgA

  • Host species

    Rabbit

  • Specificity

    ab193189 reacts with Human IgA, including both IgA1 and IgA2. No cross reactivity with Human IgG, IgM, IgD, or IgE.

  • Tested applications

    Suitable for: ELISA, IHC-P, Sandwich ELISAmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length protein corresponding to Human Human IgA.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    Purified from an animal origin–free culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    RM128

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab193189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 10 - 200 µg/ml.
IHC-P Use a concentration of 0.5 - 2 µg/ml.
Sandwich ELISA Use a concentration of 0.05 - 0.2 µg/ml.

Target

  • Relevance

    Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system.

  • Cellular localization

    Secreted

  • Database links

    • Alternative names

      • FLJ14473 antibody
      • FLJ35065 antibody
      • FLJ35500 antibody
      • FLJ36402 antibody
      • FLJ39698 antibody
      • FLJ40001 antibody
      • FLJ41548 antibody
      • FLJ41552 antibody
      • FLJ41789 antibody
      • FLJ43248 antibody
      • FLJ43594 antibody
      • FLJ44293 antibody
      • FLJ46028 antibody
      • FLJ46621 antibody
      • FLJ46724 antibody
      • IgA1 antibody
      • Igh2 antibody
      • IGHA1 antibody
      • IGHA2 antibody
      • Immunoglobulin heavy constant alpha 1 antibody
      • Immunoglobulin heavy constant alpha 2 (A2m marker) antibody
      • Immunoglobulin heavy constant alpha 2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgA antibody [RM128] (ab193189)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgA antibody [RM128] (ab193189)

      Immunohistochemical analysis of human lymphoid tissue labelling IgA with ab193189 at 0.1 - 1µg/mL.

    • Sandwich ELISA - Anti-Human IgA antibody [RM128] (ab193189)
      Sandwich ELISA - Anti-Human IgA antibody [RM128] (ab193189)

      Sandwich ELISA of Human IgA using ab193189 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

    • Sandwich ELISA - Anti-Human IgA antibody [RM128] (ab193189)
      Sandwich ELISA - Anti-Human IgA antibody [RM128] (ab193189)

      Sandwich ELISA of Human IgA using ab193189 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

    • ELISA - Anti-Human IgA antibody [RM128] (ab193189)
      ELISA - Anti-Human IgA antibody [RM128] (ab193189)

      ELISA analysis of Human immunoglobulins coated at 50 ng/well. ab193189 at 200 ng/mL, 50 ng/mL,
      or 10 ng/mL was used as the primary antibody and alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. the results shows taht ab193189 reacted to both Human IgA1 & IgA2. There was no cross reactivity with Human IgG, IgM, IgD, or IgE.

    • ELISA - Anti-Human IgA antibody [RM128] (ab193189)
      ELISA - Anti-Human IgA antibody [RM128] (ab193189)

      ELISA analysis of  different amounts of Human IgA detected using a serial dilution of ab193189 as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.

    • Anti-Human IgA antibody [RM128] (ab193189)
      Anti-Human IgA antibody [RM128] (ab193189)

    References (2)

    Publishing research using ab193189? Please let us know so that we can cite the reference in this datasheet.

    ab193189 has been referenced in 2 publications.

    • Puligedda RD  et al. Human IgA Monoclonal Antibodies That Neutralize Poliovirus, Produced by Hybridomas and Recombinant Expression. Antibodies (Basel) 9:N/A (2020). PubMed: 32121092
    • Li W  et al. Radiation therapy-induced reactive oxygen species specifically eliminates CD19+IgA+ B cells in nasopharyngeal carcinoma. Cancer Manag Res 11:6299-6309 (2019). PubMed: 31372036

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