Overview

  • Product name

    Human IgA ELISA Kit
    See all IgA kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 10%
  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.5 ng/ml
  • Range

    1.56 ng/ml - 100 ng/ml
  • Recovery

    97 %

  • Assay time

    4h 0m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human IgA in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IgA in plasma, serum, urine, saliva, milk, and cell culture supernatants.


    An IgA specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IgA specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IgA captured in plate.


    Get higher sensitivity in only 90 minutes with Human IgA ELISA Kit (ab196263) from our SimpleStep ELISA® range.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human IgA Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    IgA Microplate (12 x 8 well strips) 1 unit
    IgA Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas

  • Relevance

    Human IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA. OR: The secretory component is a component of immunoglobulin A (IgA) which consists of a portion of the polymeric immunoglobulin receptor. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen.
  • Alternative names

    • Hepatocellular carcinoma-associated protein TB6
    • Ig alpha 1 chain C region
    • Ig alpha 2 chain C region
    • IGHA
    • IGHA1
    • IGHA2
    • IGHAC
    • Immunoglobulin heavy constant alpha 1
    • Immunoglobulin heavy constant alpha 2
    • Immunoglobulin heavy constant alpha 2 A2m marker
    • PIgR
    • Poly-Ig receptor
    • polymeric immunoglobulin receptor
    • polymeric immunoglobulin receptor Secretory component
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab137980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • IgA measured in biological fluids, background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab137980

Protocols

References

This product has been referenced in:

  • Li H  et al. Tripterygium Wilfordii inhibits tonsillar IgA production by downregulating IgA class switching in IgA nephropathy. Oncotarget 8:109027-109042 (2017). Read more (PubMed: 29312588) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Seric IgA in an allergic patient

Excellent Excellent 5/5 (Ease of Use)
Abreviews
We measured IgA in serum from an allergic patient. We performed two dilutions of the same serum: 1/100000 and 1/200000 and both were in a good range (we obtained a concentration of 1,7mg/ml and 1,6mg/ml, repectively). Abcam recomend a dilution of 80000, but if necessary dilute the samples from 20000 to 200000.

Abcam user community

Verified customer

Submitted May 29 2019

Question
Answer

There is no cross-reactivity with mouse samples with this kit.

Read More

Answer

The following kits are compatible as per your request;

https://www.abcam.com/iga-human-elisa-kit-ab137980.html

https://www.abcam.com/igg-human-elisa-kit-ab100547.html

https://www.abcam.com/igm-human-elisa-kit-ab137982.html

Read More

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