Key features and details
- Sensitivity: 0.35 ng/ml
- Range: 1.56 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman IgA ELISA Kit
See all IgA kits
Intra-assay Sample n Mean SD CV% Overall 5% Inter-assay Sample n Mean SD CV% Overall 9.4%
Sample typeCell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
Assay typeSandwich (quantitative)
Range1.56 ng/ml - 100 ng/ml
Assay time4h 0m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Human IgA in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IgA in plasma, serum, urine, saliva, milk, and cell culture supernatants.
An IgA specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IgA specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IgA captured in plate.
Get higher sensitivity in only 90 minutes with Human IgA ELISA Kit (ab196263) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human IgA Antibody 1 x 120µl Chromogen Substrate 1 x 7ml IgA Microplate (12 x 8 well strips) 1 unit IgA Standard (Lyophilized) 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml
RelevanceHuman IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA. OR: The secretory component is a component of immunoglobulin A (IgA) which consists of a portion of the polymeric immunoglobulin receptor. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen.
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ab137980 has been referenced in 2 publications.
- Rojas OL et al. Recirculating Intestinal IgA-Producing Cells Regulate Neuroinflammation via IL-10. Cell 176:610-624.e18 (2019). PubMed: 30612739
- Li H et al. Tripterygium Wilfordii inhibits tonsillar IgA production by downregulating IgA class switching in IgA nephropathy. Oncotarget 8:109027-109042 (2017). PubMed: 29312588