Recombinant Anti-Human IgA1 antibody [RM124] (ab193187)
![Sandwich ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)](https://www.abcam.com/ps/products/193/ab193187/Images/ab193187-230341-anti-human-iga1-antibody-rm124-sandwich-elisa.jpg "Sandwich ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM124] to Human IgA1
- Suitable for: Sandwich ELISA, ELISA
- Reacts with: Human
Overview
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Product name
Anti-Human IgA1 antibody [RM124] -
Description
Rabbit monoclonal [RM124] to Human IgA1 -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISA, ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Human Human IgA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM124 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA |
Use a concentration of 0.05 - 0.2 µg/ml.
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| ELISA |
Use at an assay dependent concentration.
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| Notes |
|---|
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Sandwich ELISA
Use a concentration of 0.05 - 0.2 µg/ml. |
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ELISA
Use at an assay dependent concentration. |
Target
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Relevance
Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. In normal adult serum, the approximate percentage composition of IgA with respect to is subclasses is IgA1:90% and IgA2:10%. In secretory IgA, the subclass proportions may approach 50:50. The clinical significance of the subclasses has yet to be determined. -
Database links
- Entrez Gene: 3493 Human
- Omim: 146900 Human
- SwissProt: P01876 Human
- Unigene: 699841 Human
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Alternative names
- FLJ14473 antibody
- FLJ35065 antibody
- FLJ35500 antibody
see all
Images
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Sandwich ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)
Sandwich ELISA using ab193187 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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![Sandwich ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)](https://www.abcam.com/ps/products/193/ab193187/Images/ab193187-230342-anti-human-iga1-antibody-rm124-sandwich-elisa.jpg)
Sandwich ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)Sandwich ELISA using ab193187 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody
as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin. -
![ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)](https://www.abcam.com/ps/products/193/ab193187/Images/ab193187-230343-anti-human-iga1-antibody-rm124-elisa.jpg)
ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)ELISA of Human immunoglobulins (the plate was coated with 50 ng/well of different immunoglobulins) using ab193187 at 200 ng/mL, 50 ng/mL, or 10 ng/mL as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody. The graph shows ab193187 only reacted to Human IgA. There is very slightly cross reactivity with IgA2. No cross reactivity occurs with Human IgG, IgM, IgD, or IgE.
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![ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)](https://www.abcam.com/ps/products/193/ab193187/Images/ab193187-230344-anti-human-iga1-antibody-rm124-elisa.jpg)
ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)A titer ELISA of Human IgA1 with ab193187 as the primary antibody. The plate was coated with Human IgA1 at different concentrations then a serial dilution of ab193187 was used as the primary antibody . An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.
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![Anti-Human IgA1 antibody [RM124] (ab193187)](https://www.abcam.com/ps/products/193/ab193187/Images/ab193187-6-benefits-of-recombinant-antibodies.png)
Anti-Human IgA1 antibody [RM124] (ab193187)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab193187 has been referenced in 2 publications.
- Aazami H et al. Local eosinophils are associated with increased IgA subclass levels in the sinonasal mucosa of chronic rhinosinusitis with polyp patients. Allergy Asthma Clin Immunol 16:30 (2020). PubMed: 32351585
- Onodera T et al. Immune-Focusing Properties of Virus-like Particles Improve Protective IgA Responses. J Immunol 203:3282-3292 (2019). PubMed: 31704880