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Immunology Immunoglobulins Heavy Chain IgA
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Recombinant

Recombinant Anti-Human IgA1 antibody [RM124] (ab193187)

  • Datasheet
  • SDS
Submit a review Submit a question References (2)

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Sandwich ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)
  • Sandwich ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)
  • ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)
  • ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)
  • Anti-Human IgA1 antibody [RM124] (ab193187)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [RM124] to Human IgA1
  • Suitable for: Sandwich ELISA, ELISA
  • Reacts with: Human

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Overview

  • Product name

    Anti-Human IgA1 antibody [RM124]
  • Description

    Rabbit monoclonal [RM124] to Human IgA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Sandwich ELISA, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human Human IgA.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RM124
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgA
    • Secondary antibodies
    • anti-Human
    • IgA
    • Unconjugated

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • Native Human IgA1 protein (ab91020)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab193187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
Use a concentration of 0.05 - 0.2 µg/ml.
ELISA
Use at an assay dependent concentration.
Notes
Sandwich ELISA
Use a concentration of 0.05 - 0.2 µg/ml.
ELISA
Use at an assay dependent concentration.

Target

  • Relevance

    Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. In normal adult serum, the approximate percentage composition of IgA with respect to is subclasses is IgA1:90% and IgA2:10%. In secretory IgA, the subclass proportions may approach 50:50. The clinical significance of the subclasses has yet to be determined.
  • Database links

    • Entrez Gene: 3493 Human
    • Omim: 146900 Human
    • SwissProt: P01876 Human
    • Unigene: 699841 Human
    • Alternative names

      • FLJ14473 antibody
      • FLJ35065 antibody
      • FLJ35500 antibody
      • FLJ36402 antibody
      • FLJ39698 antibody
      • FLJ40001 antibody
      • FLJ41548 antibody
      • FLJ41552 antibody
      • FLJ41789 antibody
      • FLJ43248 antibody
      • FLJ43594 antibody
      • FLJ44293 antibody
      • FLJ46028 antibody
      • FLJ46621 antibody
      • FLJ46724 antibody
      • FLJ46811 antibody
      • FLJ46824 antibody
      • FLJ90170 antibody
      • Ig alpha 1 chain C region antibody
      • IgA1 antibody
      • IGHA1 antibody
      • immunoglobulin heavy constant alpha 1 antibody
      • MGC102857 antibody
      see all

    Images

    • Sandwich ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)
      Sandwich ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)

      Sandwich ELISA using ab193187 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

    • Sandwich ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)
      Sandwich ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)

      Sandwich ELISA using ab193187 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody
      as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

    • ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)
      ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)

      ELISA of Human immunoglobulins (the plate was coated with 50 ng/well of different immunoglobulins) using ab193187 at 200 ng/mL, 50 ng/mL, or 10 ng/mL as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody. The graph shows ab193187 only reacted to Human IgA. There is very slightly cross reactivity with IgA2. No cross reactivity occurs with Human IgG, IgM, IgD, or IgE. 

    • ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)
      ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)

      A titer ELISA of Human IgA1 with ab193187 as the primary antibody. The plate was coated with Human IgA1 at different concentrations then a serial dilution of ab193187 was used as the primary antibody . An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.

    • Anti-Human IgA1 antibody [RM124] (ab193187)
      Anti-Human IgA1 antibody [RM124] (ab193187)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab193187? Please let us know so that we can cite the reference in this datasheet.

    ab193187 has been referenced in 2 publications.

    • Aazami H  et al. Local eosinophils are associated with increased IgA subclass levels in the sinonasal mucosa of chronic rhinosinusitis with polyp patients. Allergy Asthma Clin Immunol 16:30 (2020). PubMed: 32351585
    • Onodera T  et al. Immune-Focusing Properties of Virus-like Particles Improve Protective IgA Responses. J Immunol 203:3282-3292 (2019). PubMed: 31704880

    Customer reviews and Q&As

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