Recombinant

Recombinant Anti-Human IgA1 antibody [RM124] (ab193187)

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Overview

  • Product name

    Anti-Human IgA1 antibody [RM124]

  • Description

    Rabbit monoclonal [RM124] to Human IgA1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Sandwich ELISA, ELISA, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length protein corresponding to Human Human IgA.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 48% PBS, 50% Glycerol
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    Purified from an animal origin–free culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    RM124

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab193187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.1 - 1 µg/ml.
ICC/IF Use a concentration of 0.5 - 2 µg/ml.
Sandwich ELISA Use a concentration of 0.05 - 0.2 µg/ml.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. In normal adult serum, the approximate percentage composition of IgA with respect to is subclasses is IgA1:90% and IgA2:10%. In secretory IgA, the subclass proportions may approach 50:50. The clinical significance of the subclasses has yet to be determined.

  • Database links

    • Alternative names

      • FLJ14473 antibody
      • FLJ35065 antibody
      • FLJ35500 antibody
      • FLJ36402 antibody
      • FLJ39698 antibody
      • FLJ40001 antibody
      • FLJ41548 antibody
      • FLJ41552 antibody
      • FLJ41789 antibody
      • FLJ43248 antibody
      • FLJ43594 antibody
      • FLJ44293 antibody
      • FLJ46028 antibody
      • FLJ46621 antibody
      • FLJ46724 antibody
      • FLJ46811 antibody
      • FLJ46824 antibody
      • FLJ90170 antibody
      • Ig alpha 1 chain C region antibody
      • IgA1 antibody
      • IGHA1 antibody
      • immunoglobulin heavy constant alpha 1 antibody
      • MGC102857 antibody
      see all

    Images

    • Sandwich ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)
      Sandwich ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)

      Sandwich ELISA using ab193187 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

    • Sandwich ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)
      Sandwich ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)

      Sandwich ELISA using ab193187 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody
      as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

    • ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)
      ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)

      ELISA of Human immunoglobulins (the plate was coated with 50 ng/well of different immunoglobulins) using ab193187 at 200 ng/mL, 50 ng/mL, or 10 ng/mL as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody. The graph shows ab193187 only reacted to Human IgA. There is very slightly cross reactivity with IgA2. No cross reactivity occurs with Human IgG, IgM, IgD, or IgE. 

    • ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)
      ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)

      A titer ELISA of Human IgA1 with ab193187 as the primary antibody. The plate was coated with Human IgA1 at different concentrations then a serial dilution of ab193187 was used as the primary antibody . An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.

    References

    ab193187 has not yet been referenced specifically in any publications.

    Publishing research using ab193187? Please let us know so that we can cite the reference in this datasheet.

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