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Abcam’s IgD Human ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgD in Human samples.
In this assay the IgD present in samples reacts with the anti-IgD antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgD antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound IgD. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgD in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgD in the test sample. The quantity of IgD in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
|Components||1 x 96 tests|
|100X HRP-conjugated anti-Human IgD antibody||1 x 150µl|
|20X Wash Buffer Concentrate||1 x 50ml|
|5X Diluent Concentrate||1 x 50ml|
|Chromogen Substrate Solution||1 x 12ml|
|Human IgD Calibrator (lyophilized)||1 vial|
|Human IgD ELISA Microplate||1 unit|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab157708 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab157708 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"