Product nameHuman IgE ELISA Kit
Sample typeSerum, Plasma
Assay typeSandwich (quantitative)
Sensitivity= 5 IU/ml
Range5 IU/ml - 800 IU/ml
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
ab108650 Immunoglobulin E (IgE) Human ELISA Kit is intended for the quantitative determination of Immunoglobulin E (IgE) concentration in Human serum.
Patients with atopic allergic diseases such as atopic asthma, atopic dermatitis, and hay fever have been shown to exhibit increased total immunoglobulin E (IgE) levels in blood. IgE is also known as the reagenic antibody. In general, elevated levels of IgE indicate an increased probability of an IgE-mediated hypersensitivity, responsible for allergic reactions. Parasitic infestations such as hookworm, and certain clinical disorders including aspergillosis, have also been demonstrated to cause high levels of IgE. Decreased levels of IgE are found in cases of hypogammaglobulinemia, autoimmune diseases, ulcerative colitis, hepatitis, cancer, and malaria. Cord blood or serum IgE levels may have prognostic value in assessing the risk of future allergic conditions in children. The IgE serum concentration in a patient is dependent on both the extent of the allergic reaction and the number of different allergens to which he is sensitized. Nonallergic normal individuals have IgE concentrations that vary widely and increase steadily during childhood, reaching their highest levels at age 15 to 20, and thereafter remaining constant until about age 60 when they slowly decline.
Tested applicationsSuitable for: Sandwich ELISAmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Enzyme Conjugate Reagent 1 x 18ml IgE Reference Standard 1 (0 IU/ml) 1 x 0.5ml IgE Reference Standard 2 (10 IU/ml) 1 x 0.5ml IgE Reference Standard 3 (50 IU/ml) 1 x 0.5ml IgE Reference Standard 4 (100 IU/ml) 1 x 0.5ml IgE Reference Standard 5 (400 IU/ml) 1 x 0.5ml IgE Reference Standard 6 (800 IU/ml) 1 x 0.5ml Monoclonal anti-IgE-coated microtiter plate 1 unit Stop Solution 1 x 11ml TMB Reagent 1 x 11ml Zero Buffer 1 x 13ml
- Constant region of heavy chain of IgE
- HEAVY EPSILON CHAIN PSEUDOGENE 1
- HEAVY EPSILON CHAIN PSEUDOGENE 2
Our Abpromise guarantee covers the use of ab108650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|
This product has been referenced in:
- Wan Z et al. Photocleavage-based affinity purification of biomarkers from serum: Application to multiplex allergy testing. PLoS One 13:e0191987 (2018). Read more (PubMed: 29389948) »
- Ramos-Martínez E et al. Reduction of respiratory infections in asthma patients supplemented with vitamin D is related to increased serum IL-10 and IFN? levels and cathelicidin expression. Cytokine 108:239-246 (2018). Read more (PubMed: 29402723) »