Overview

  • Product name

    Human IgE ELISA Kit
  • Detection method

    Colorimetric
  • Sample type

    Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 5 IU/ml
  • Range

    5 IU/ml - 800 IU/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    ab108650 Immunoglobulin E (IgE) Human ELISA Kit is intended for the quantitative determination of Immunoglobulin E (IgE) concentration in Human serum.

    Patients with atopic allergic diseases such as atopic asthma, atopic dermatitis, and hay fever have been shown to exhibit increased total immunoglobulin E (IgE) levels in blood. IgE is also known as the reagenic antibody. In general, elevated levels of IgE indicate an increased probability of an IgE-mediated hypersensitivity, responsible for allergic reactions. Parasitic infestations such as hookworm, and certain clinical disorders including aspergillosis, have also been demonstrated to cause high levels of IgE. Decreased levels of IgE are found in cases of hypogammaglobulinemia, autoimmune diseases, ulcerative colitis, hepatitis, cancer, and malaria. Cord blood or serum IgE levels may have prognostic value in assessing the risk of future allergic conditions in children. The IgE serum concentration in a patient is dependent on both the extent of the allergic reaction and the number of different allergens to which he is sensitized. Nonallergic normal individuals have IgE concentrations that vary widely and increase steadily during childhood, reaching their highest levels at age 15 to 20, and thereafter remaining constant until about age 60 when they slowly decline.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    Enzyme Conjugate Reagent 1 x 18ml
    IgE Reference Standard 1 (0 IU/ml) 1 x 0.5ml
    IgE Reference Standard 2 (10 IU/ml) 1 x 0.5ml
    IgE Reference Standard 3 (50 IU/ml) 1 x 0.5ml
    IgE Reference Standard 4 (100 IU/ml) 1 x 0.5ml
    IgE Reference Standard 5 (400 IU/ml) 1 x 0.5ml
    IgE Reference Standard 6 (800 IU/ml) 1 x 0.5ml
    Monoclonal anti-IgE-coated microtiter plate 1 unit
    Stop Solution 1 x 11ml
    TMB Reagent 1 x 11ml
    Zero Buffer 1 x 13ml
  • Research areas

  • Alternative names

    • Constant region of heavy chain of IgE
    • HEAVY EPSILON CHAIN PSEUDOGENE 1
    • HEAVY EPSILON CHAIN PSEUDOGENE 2
    • Ig epsilon chain C region
    • IgE IMMUNOGLOBULIN
    • Igh2
    • IGHE
    • IGHEP1
    • IGHEP2
    • Immunoglobulin epsilon
    • Immunoglobulin heavy chain (epsilon polypeptide)
    • Immunoglobulin heavy constant epsilon
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

  • IgE measured in biological fluids showing quantity (IU) per mL of tested sample. Samples were diluted 1-2 fold.

  • Results of a typical standard run with absorbency readings at 450nm shown on the Y axis against IgE concentrations shown on the X axis.

Protocols

References

This product has been referenced in:

  • Wan Z  et al. Photocleavage-based affinity purification of biomarkers from serum: Application to multiplex allergy testing. PLoS One 13:e0191987 (2018). Read more (PubMed: 29389948) »
  • Ramos-Martínez E  et al. Reduction of respiratory infections in asthma patients supplemented with vitamin D is related to increased serum IL-10 and IFN? levels and cathelicidin expression. Cytokine 108:239-246 (2018). Read more (PubMed: 29402723) »
See all 5 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

LABEL FAULT !

Average Good 4/5 (Ease of Use)
Abreviews
We did the experiment with the standard protocol, but the experiment was failed.
So we did the troubleshooting experiment and found the label fault.

The TMB reagent label was attached on the bottle of enzyme conjugate reagent, and the enzyme conjugate reagent label was attached on the bottle of TMB reagent!

Our important samples was wasted !

We contacted the Korean technical support team, and complained this problem.

There is some doubt about every kit, and researchers should check the label of reagent before do the experiment!

Note from Abcam Scientific Support - We are very sorry for this mislabeling and sincerely regret the loss of samples. To help resolve this issue we have provided a new kit free of charge.

Abcam user community

Verified customer

Submitted Jul 24 2013

Answer

Thank you for contacting us.
The Human IgE ELISA kit ab108650 has only been tested in human serum.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for contacting us. You do not need any additional reagents apart from those listed on pages 5 and 6. The rinsing is done with the distilled/deionozed water.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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