Human IgE full length protein (Azide-free) (ab65866)

Overview

  • Product name
    Human IgE full length protein (Azide-free)
    See all IgE proteins and peptides
  • Protein length
    Full length protein
  • Description
    Native Human IgE protein (Azide free)

Description

  • Nature
    Native
  • Source
    Native
  • Amino Acid Sequence
    • Species
      Human
    • Additional sequence information
      Observed band of ~185kDa on SDS-PAGE

Specifications

Our Abpromise guarantee covers the use of ab65866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    ELISA

    Immunocytochemistry

  • Endotoxin level
    < 25.000 Eu/mg
  • Purity
    > 90 % SDS-PAGE.
    Human IgE full length protein was purified by Protein L chromatography. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The remaining contaminants are mainly components from Foetal Bovine Serum.
  • Form
    Liquid
  • Additional notes

    This product is purified Human IgE with kappa light chains produced in vitro from a monoclonal hybridoma.

    This antigen is quite unique, because it is the only available IgE product, which originates from a human monoclonal cell line. Our IgE product shows superior advantage when compared to the traditionally purified polyclonal IgE from myeloma patients. A comparison which is highly superior on the following key parameters:.

    • Less batch-to-batch variation
    • Higher degree of purity (>90%)
    • A more uniform product (monoclonal and not polyclonal)

     

    IgE has been used in a variety of cell stimulation assays, including the following cell types:

    • Human airway smooth muscle (HASM) cells
    • mast cells (MC)
    • Human bronchial/tracheal smooth muscle (B/TSM) cells
    • acute myelogenous leukemia (AML) blasts
    • Human neutrophils

     

    1 mg of ab65866 is 380,000± 50,000 IU/ml.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: 0.0268% PBS, 0.87% Sodium chloride

General Info

  • Alternative names
    • Igh2
    • IGHE
    • IGHEP1
    • Immunoglobulin heavy constant epsilon
  • Relevance
    IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • Cellular localization
    Cell Membrane

Images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • SDS-PAGE analysis of:

    Lane 1: Molecular weight markers.
    Lane 2: ab65866.
    Lane 3: IgE purified from serum from a myeloma patient.

References

This product has been referenced in:
  • Vidal M  et al. Development of quantitative suspension array assays for six immunoglobulin isotypes and subclasses to multiple Plasmodium falciparum antigens. J Immunol Methods N/A:N/A (2018). Read more (PubMed: 29397157) »
  • Wan Z  et al. Photocleavage-based affinity purification of biomarkers from serum: Application to multiplex allergy testing. PLoS One 13:e0191987 (2018). Read more (PubMed: 29389948) »

See all 7 Publications for this product

Customer reviews and Q&As

The masses in the molecular weight marker are as follows, heaviest to lightest in kDa:

1. 195
2. 142
3. 96
4. 71 (Dark band)
5. 48
6. 33
7. 28
8. 22

I am sorry to confirm we are not able to sell this antibody at 1 mg/ml. I can suggest to consider trying concentrating the protein once you receive it.

Please note that as far as we are aware it has not been tested by the application you wis...

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The lab let me know that they do not measure endotoxin levels in any of their protein solutions and hence they cannot give us a specific value for ab65866.

I can confirm that ab65866 Human IgE protein has a purity of >95% as determined by SDS PAGE.
I am sorry it has not been given a purity 'grading'.

Yes, ab65866 is produced from the HE1 clone.

The antigen is produced in a monoclonal hybridoma, and is not purified from blood or anything else, so the concentration is determined by measuring OD 280. It is then sold based on mg/mL and not in units as the WHO standard product.

The ab65866 Human IgE protein is an affinity-purified, fully human IgE antibody with kappa light chains. The IgE is produced in vitro from a monoclonal hybridoma. The hybridoma is made by purifying IgE producing B cells from a patient with multiple Mye...

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Yes, this protein can stimulate mast cells.

We can confirm that the human IgE have kappa light chains.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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