Overview

  • Product name

    Human IGF2R ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 1.8ng/ml 0.063 3.5%
    2 16 4.2ng/ml 0.172 4.1%
    3 16 6.7ng/ml 0.389 5.8%
    Inter-assay
    Sample n Mean SD CV%
    1 24 1.5ng/ml 0.065 4.3%
    2 24 3.9ng/ml 0.222 5.7%
    3 24 6.4ng/ml 0.397 6.2%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    156 pg/ml - 10000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human IGF2R ELISA Kit (ab213788) is designed for the quantitative measurement of Human IGF2R in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for IGF2R has been precoated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: S1510-F2108) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGF2R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IGF2R amount of sample captured in plate. 

  • Notes

    Insulin-like growth factor 2 receptor, also called IGF2R or I-MPR is a protein that in humans is encoded by the IGF2R gene. This gene is mapped to 6q25.3. This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate, although the binding sites for either are located on different segments of the receptor. This receptor functions in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. While the related mouse gene shows exclusive expression from the maternal allele, imprinting of the human gene appears to be polymorphic, with only a minority of individuals showing expression from the maternal allele.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human IGF2R coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Human IGF2R antibody 1 x 130µl
    Lyophilized recombinant Human IGF2R standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.
  • Sequence similarities

    Belongs to the MRL1/IGF2R family.
    Contains 1 fibronectin type-II domain.
  • Domain

    Contains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends.
  • Cellular localization

    Lysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface.
  • Information by UniProt
  • Alternative names

    • 300 kDa mannose 6 phosphate receptor
    • 300 kDa mannose 6-phosphate receptor
    • Cation independent mannose 6 phosphate receptor
    • Cation-independent mannose-6-phosphate receptor
    • CD222
    • CD222 antigen
    • CI Man 6 P receptor
    • CI Man-6-P receptor
    • CI MPR
    • CI-M6PR
    • CI-MPR
    • CIMPR
    • IGF 2 receptor
    • IGF 2R
    • IGF II receptor
    • IGF-II receptor
    • IGF2 receptor
    • Igf2r
    • Insulin like growth factor 2 receptor
    • Insulin like growth factor II receptor
    • Insulin-like growth factor 2 receptor
    • Insulin-like growth factor II receptor
    • M6P R
    • M6P/IGF2 receptor
    • M6P/IGF2R
    • M6PR
    • mannose 6 phosphate receptor
    • mannose 6 phosphate receptor, cation independent
    • MPR 300
    • MPR300
    • MPRI
    • MPRI_HUMAN
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human IGF2R ELISA Kit (ab213788) Standard Curve

Protocols

References

ab213788 has not yet been referenced specifically in any publications.

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