Human IGFBP-1 ELISA Kit (ab213789)
- Datasheet
- References
- Protocols
Overview
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Product name
Human IGFBP-1 ELISA Kit
See all IGFBP1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 145pg/ml 6.09 4.2% 2 16 736pg/ml 37.54 5.1% 3 16 1243pg/ml 70.85 5.7% Inter-assay Sample n Mean SD CV% 1 24 173pg/ml 10.21 5.9% 2 24 667pg/ml 42.02 6.3% 3 24 1360pg/ml 102 7.5% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 1 pg/ml -
Range
31.2 pg/ml - 2000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The Human IGFBP-1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213789) is designed for the quantitative detection of Human IGFBP-1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for IGFBP-1 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: A26-N259) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IGFBP-1 amount of sample captured in plate. The standard product used in this kit is recombinant human IGFBP-1, consisting of 234 amino acids with the molecular mass of 25 kDa.
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Notes
IGFBP1 is also known as amniotic fluid binding protein (AFBP), placental protein-12, alpha-pregnancy associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25. The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb. The IBP-1 gene is a single copy gene, located on chromosome 7.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human IGFBP-1 coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti- Human IGFBP-1 antibody 1 x 130µl Lyophilized recombinant Human IGFBP-1 standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
Research areas
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Function
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. -
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
Post-translational
modificationsPhosphorylated; probably by casein kinase II. Alters the affinity of the protein for IGFs. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- AFBP
- Alpha pregnancy associated endometrial globulin
- Amniotic fluid binding protein
see all -
Database links
- Entrez Gene: 3484 Human
- Omim: 146730 Human
- SwissProt: P08833 Human
- Unigene: 642938 Human
Images
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Human IGFBP-1 ELISA Kit (ab213789) Standard CurveHuman IGFBP-1 ELISA Kit (ab213789) Standard Curve
Protocols
References
ab213789 has not yet been referenced specifically in any publications.
