Product nameHuman IGFBP1 ELISA Kit
Sample typeCell culture supernatant, Serum, Plasma, Cell Lysate
Assay typeSandwich (quantitative)
Sensitivity< 5 pg/ml
Range2.74 pg/ml - 2000 pg/ml
> 92 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 94.64 83% - 102% Serum 95.27 84% - 104% Plasma 92.47 82% - 102%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s IGFBP1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IGFBP1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human IGFBP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGFBP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples
Tested applicationsSuitable for: Sandwich ELISAmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 240X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human IGFBP1 (lyophilized) 2 vials IGFBP1 Microplate (12 x 8 wells) 1 unit Recombinant Human IGFBP1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
RelevanceInsulin like growth factor binding protein 1 (IGFBP1) is a member of the superfamily of insulin like growth factor (IGF) binding proteins which include six high affinity IGF binding proteins (IGFBP) and at least four low affinity binding proteins referred to as IGFBP related proteins (IGFBPrP). The IGFBP members are cysteine rich proteins with conserved cysteine residues clustered in the amino terminal and the carboxy terminal regions of the molecule. The N terminal and C terminal regions are highly homologous among rat, human and bovine sequences. Contained within IGFBP1 and 2 is an integrin receptor recognition sequence (RGD) that is responsible for promoting cell migration by an IGF independent action. IGFBPs hold a central position in IGF ligand receptor interactions through influences on both the bioavailability and distribution of IGFs in the extracellular environment. IGFBPs will either inhibit or enhance the biological activities of IGF or act in an IGF independent manner.
Our Abpromise guarantee covers the use of ab100539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
This product has been referenced in:
- Mohr EL et al. Ocular and uteroplacental pathology in a macaque pregnancy with congenital Zika virus infection. PLoS One 13:e0190617 (2018). Read more (PubMed: 29381706) »
- Gligorijevic N et al. Structural changes of fibrinogen as a consequence of cirrhosis. Thromb Res 166:43-49 (2018). Read more (PubMed: 29655002) »