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We tested TMB and HRP-streptavidin activity, as you recommended, and we have very weak blue colour intensity. Our institute also develops tests based on immunoaffinity for years, and our results more looks like a having problem with test sensitivity.We tested also TMB from this kit with HRP-labelled IgG from some other kit just to see the colour intensity, and developed colour was of much more intensity than that developed with HRP-streptavidin from ELISA IGFBP-1 kit. We was aware of all storage instructions before starting our experiments.
Asked on Jan 20 2012
Thank you for your message and cooperation, and for kindlyproviding this further information.
Reviewing the details, I appreciate the time you have spent on these experiments and would be pleased to arrange afree of charge replacementor credit note in compensation in this case.
I look forward to hearing from you with details of how you would like to proceed.
Answered on Jan 20 2012