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I would like to know whether there is cross-reactivity with murine IGFBP-1 levels in the ELISA kit as I would like to know the total level of IGFBP-1. IGFBP1 Human ELISA Kit (ab100539)
Asked on Oct 07 2013
The immunogen used to raise the antibody pair in IGFBP1 Human ELISA Kit was a recombinant human IGFBP-1, aa 26-259. (This is the full length protein minus the signal peptide).
Unfortunately, epitope mapping has not been performed so it is not known where exactly in this region the antibody pair is binding. However, based on our cross reactivity tests, this assay cross reacts ˜10% with recombinant mouse IGF-1. Therefore based on this data, it is unlikely that ab100539 will work in mouse.
Elisa ThomasAbcam Scientific Support
Answered on Oct 07 2013