Overview

  • Product name

    Human IGFBP1 ELISA Kit
    See all IGFBP1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Supernatant 5 5.1%
    Inter-assay
    Sample n Mean SD CV%
    Supernatant 3 6.3%
  • Sample type

    Cell culture supernatant, Milk, Urine, Serum, Cell culture extracts, Hep Plasma, EDTA Plasma, Cit plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    11.6 pg/ml
  • Range

    17.2 pg/ml - 1100 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Milk 101 90% - 119%
    Urine 97 91% - 109%
    Serum 99 94% - 104%
    Cell culture extracts 100 89% - 112%
    Hep Plasma 102 97% - 108%
    EDTA Plasma 103 97% - 111%
    Cit plasma 104 101% - 108%
    Tissue Homogenate 101 93% - 108%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    IGFBP1 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IGFBP1 protein in human serum, plasma, urine, milk, cell and tissue extract, and cell supernatant samples


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    IGFBP1 (insulin growth factor binding protein 1) is an IGF-1 and IGF-2 binding protein involved in the stabilization of IGF-1 and IGF-2 half-lives. IGFBP-1 can both promote and inhibit IGF effects. IGFBP1 is primarily made in the liver and kidney, and altered expression is associated with several pathologies including diabetes development and coronary heart disease.


    Sensitivity
    Samples diluted in Sample Diluent NS - 11.6 pg/ml
    Samples diluted in 1X Cell Extraction Buffer - 2.9 pg/ml

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human IGFBP1 Capture Antibody 1 x 600µl
    10X Human IGFBP1 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BI 1 x 6ml
    Human IGFBP1 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.
  • Sequence similarities

    Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • Post-translational
    modifications

    Phosphorylated; probably by casein kinase II. Alters the affinity of the protein for IGFs.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • AFBP
    • Alpha pregnancy associated endometrial globulin
    • Amniotic fluid binding protein
    • Binding protein 25
    • Binding protein 26
    • Binding protein 28
    • Growth hormone independent binding protein
    • hIGFBP 1
    • hIGFBP1
    • IBP 1
    • IBP-1
    • IBP1
    • IBP1_HUMAN
    • IGF binding protein 1
    • IGF BP25
    • IGF-binding protein 1
    • IGFBP 1
    • IGFBP-1
    • IGFBP1
    • Insulin like growth factor binding protein 1
    • Insulin-like growth factor-binding protein 1
    • Placental protein 12
    • PP 12
    • PP12
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab233618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between Human IGFBP1 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • The IGFBP1 standard curve was prepared as described in Section 10.

  • The concentrations of IGFBP1 were measured in duplicates, interpolated from the IGFBP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2.5%, plasma (citrate) 5%, plasma (heparin) 5%, and plasma (EDTA) 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP1 concentration was determined to be 22 ng/mL in serum, 16 ng/mL in plasma (citrate), 21 ng/ml in plasma (heparin), and 37 ng/ml in plasma (EDTA).

  • The concentrations of IGFBP1 were measured in duplicates, interpolated from the IGFBP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: milk 5%, A549 supernatant 100%, and HepG2 supernatant 0.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP1 concentration was determined to be 12 ng/ml in milk, 0.5 ng/ml in A549 supernatant, and 172 ng/mL in HepG2 supernatant.

  • The concentrations of IGFBP1 were measured in duplicate and interpolated from the IGFBP1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP1 concentration was determined to be 580 pg/mL in HepG2 cell extract, 457 pg/ml in U-87 MG cell extract, and 739 pg/mL in liver tissue homogenate extract samples.

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP1 concentration was determined to be 29.1 ng/mL with a range of 1.5 – 117.3 ng/mL.

Protocols

References

ab233618 has not yet been referenced specifically in any publications.

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