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Dear Sir or Madame, I ordered at your company three ELISA kits for the detection of human IGFBP2 (ab100540), IGFBP3 (100541) and IGFBP5 (100543). I am satisfied with these products. Only do I have a question I could not find the answer in the instructions manual. Do the detection antibodies for IGFBP2, IGFBP3 and IGFBP5 used in these kits also recognize cleaved forms of these proteins or only the full length proteins? I thank you in advance for your response. Best regards,
Asked on Oct 17 2011
Thank you for your patient. I just had a response from my lab colleagues. They confirmed that all three kits were designed against the mature form of the protein (which is the full amino acid sequence minus the signalling peptide). As the epitope is located somewhere on the mature form, the detection antibodies will both recognise uncleaved and cleaved forms of the protein. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Oct 17 2011