Product nameHuman IGFBP3 Matched Antibody Pair Kit
See all IGFBP3 kits
Assay typeELISA set
Range62.5 pg/ml - 4000 pg/ml
Species reactivityReacts with: Human
Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant human IGFBP3.
Both capture and detector antibodies are rabbit monoclonal antibodies.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Additional protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website.
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab211652) which uses the same antibody pair.
Buffer information: The capture antibody is glycerol free. The detector antibody contains glycerol.
To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.
Tested applicationsSuitable for: ELISAmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 10 x 96 tests 2 x 96 tests Human IGFBP3 Capture Antibody 1 x 100µg 1 x 20µg Human IGFBP3 Detector Antibody 1 x 25µg 1 x 5µg Human IGFBP3 Lyophilized Protein 1 vial 1 vial
FunctionIGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Tissue specificityExpressed by most tissues. Present in plasma.
Sequence similaritiesContains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain.
Developmental stageIGFBP3 levels are higher during extrauterine life and peak during puberty.
DomainThe thyroglobulin type-1 domain mediates interaction with HN.
modificationsPhosphorylation sites are present in the extracelllular medium.
- Information by UniProt
- Acid stable subunit of the 140 K IGF complex
- Binding protein 29
- Binding protein 53
SimpleStep ELISA kits
Our Abpromise guarantee covers the use of ab217607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
ab217607 has not yet been referenced specifically in any publications.