Overview

  • Product name

    Human IGFBP4 ELISA Kit
    See all IGFBP4 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Plasma 5 4.8%
    Inter-assay
    Sample n Mean SD CV%
    Plasma 3 6.1%
  • Sample type

    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    12 pg/ml
  • Range

    39.1 pg/well - 2500 pg/well
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 107 105% - 110%
    Serum 114 106% - 125%
    Heparin Plasma 101 99% - 101%
    EDTA Plasma 112 101% - 127%
    Citrate Plasma 108 101% - 120%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    IGFBP4 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IGFBP4 protein in human serum, plasma, and cell culture supernatant.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    IGFBP4 (insulin-like growth factor-binding protein 4) is a member of IGF-binding proteins. IGFBP4 modulates the interaction of IGF-I and IGF-2 with their receptors and extends the half-life of these proteins.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab230936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • The IGFBP4 standard curve was prepared as described in Section 10.

  • The concentrations of IGFBP4 were measured in duplicates, interpolated from the IGFBP4 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:200, plasma (citrate) 1:200, plasma (heparin) 1:200, plasma (EDTA) 1:200, and A549 supernatant 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP4 concentration was determined to be 452 ng/mL in serum, 336 ng/mL in plasma (citrate), 236 ng/ml in plasma (heparin), 359 ng/ml in plasma (EDTA), and 410 ng/mL in A549 supernatant.

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP4 concentration was determined to be 478 ng/mL with a range of 389 – 743 ng/mL.

Protocols

References

ab230936 has not yet been referenced specifically in any publications.

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