Human IGFBP6 ELISA Kit (ab240686)
- Datasheet
- References
- Protocols
Overview
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Product name
Human IGFBP6 ELISA Kit
See all IGFBP6 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.3% Inter-assay Sample n Mean SD CV% Serum 3 3.9% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
4.03 pg/ml -
Range
7.81 pg/ml - 500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 99 91% - 106% Cell culture media 100 98% - 104% Hep Plasma 106 99% - 117% EDTA Plasma 99 96% - 102% Cit plasma 98 90% - 108% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Cow, Human -
Product overview
IGFBP6 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IGFBP6 protein in human serum, plasma, and cell culture supernatant.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
The superfamily of insulinlike growth factor (IGF) binding proteins include the six highaffinity IGF binding proteins (IGFBP) and at least four additional lowaffinity binding proteins referred to as IGFBP related proteins (IGFBPrP). IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Posttranslational modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.
Human IGFBP6 encodes a 240-amino acid (aa) residue precursor protein with a 24-aa residue signal peptide that is processed to generate the 216-aa Oglycosylated mature protein. IGFBP6 is expressed in ovarian cells, prostatic cells, and fibroblasts and is found predominantly in CSF and serum. IGFBP6 binds preferentially to IGFII, exhibiting a 2-fold higher affinity for IGFII than for IGFI.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IGFBP6 Capture Antibody 1 x 600µl 10X Human IGFBP6 Detector Antibody 2 vials 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human IGFBP6 Lyophilized Recombinant Protein 1 x 600µl Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
Post-translational
modificationsO-linked glycans consist of hexose (probably Gal), N-acetylhexosamine (probably GalNAc) and sialic acid residues. O-glycosylated with core 1 or possibly core 8 glycans. O-glycosylated on one site only in the region AA 143-168 in cerebrospinal fluid. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IBP 6
- IBP-6
- IBP6
see all -
Database links
- Entrez Gene: 404186 Cow
- Entrez Gene: 3489 Human
- Omim: 146735 Human
- SwissProt: Q05718 Cow
- SwissProt: P24592 Human
- Unigene: 274313 Human
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab240686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Other - Human IGFBP6 ELISA Kit (ab240686)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human IGFBP6 standard curve in Sample Diluent NS.The IGFBP6 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native IGFBP6 in human serum and plasma samples.The concentrations of IGFBP6 were measured in duplicates, interpolated from the IGFBP6 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 0.25%, plasma (citrate) 0.25%, plasma (EDTA) 0.25%, and plasma (heparin) 0.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP6 concentration was determined to be 362.78 ng/mL in serum, 276.59 ng/mL in plasma (citrate), 388.32 ng/mL plasma (EDTA), and 360.96 ng/mL plasma (Heparin).
Protocols
References
ab240686 has not yet been referenced specifically in any publications.
