Overview

  • Product name

    Human IGFBP7 ELISA Kit
    See all IGFBP7 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 2.3ng/ml 0.101 4.4%
    2 16 16.2ng/ml 0.729 4.5%
    3 16 28.1ng/ml 1.43 5.1%
    Inter-assay
    Sample n Mean SD CV%
    1 24 2.8ng/ml 0.188 6.7%
    2 24 15.4ng/ml 1.063 6.9%
    3 24 29.7ng/ml 2.14 7.2%
  • Sample type

    Cell culture supernatant, Urine, Serum, Heparin Plasma, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 20 pg/ml
  • Range

    625 pg/ml - 40000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human IGFBP7 ELISA Kit (ab213790) is designed for the quantitative measurement of Human IGFBP7 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for IGFBP7 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: D30-L282) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IGFBP7 amount of sample captured in plate. 

  • Notes

    Insulin-like growth factor-binding protein 7 is a protein that in humans is encoded by the IGFBP7 gene. The gene was mapped to chromosome 4q12. The major function of the protein is the regulation of availability of insulin-like growth factors (IGF's) in tissue as well as in modulating IGF binding to its receptors. IGFBP7 binds to IGF with high affinity. It also stimulates cell adhesion. The protein is implicated in some cancers.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human IGFBP7 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Human IGFBP7 antibody 1 x 130µl
    Lyophilized recombinant Human IGFBP7 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion.
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 Kazal-like domain.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • AGM
    • Angiomodulin
    • FSTL2
    • IBP-7
    • IBP7
    • IBP7_HUMAN
    • IGF binding protein 7
    • IGF-binding protein 7
    • IGFBP rP1
    • IGFBP-7
    • IGFBP-rP1
    • IGFBP7
    • IGFBPRP1
    • Insulin like growth factor binding protein 7
    • Insulin-like growth factor-binding protein 7
    • MAC25
    • MAC25 protein
    • PGI2 stimulating factor
    • PGI2-stimulating factor
    • Prostacyclin stimulating factor
    • Prostacyclin-stimulating factor
    • PSF
    • RAMSVPS
    • TAF
    • Tumor-derived adhesion factor
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human IGFBP7 ELISA Kit (ab213790) Standard Curve

Protocols

References

ab213790 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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