Anti-Human IgG antibody [2A11] (ab7497)

Submit a review Q&A (3)

Overview

  • Product name
    Anti-Human IgG antibody [2A11]
    See all Human IgG primary antibodies
  • Description
    Mouse monoclonal [2A11] to Human IgG
  • Host species
    Mouse
  • Specificity
    Ab7497 specifically recognises Hinge region of Human IgG. No cross-reactivity with IgA,IgM,IgE.
  • Tested applications
    Suitable for: ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    These clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunised with IgG from human serum.

  • General notes

    Concentration varies from lot to lot and can be provided on request.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab109489 as a replacement.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    2A11
  • Myeloma
    Sp2/0
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
  • Cellular localization
    Cell Membrane and Secreted
  • Database links
    see all
  • Alternative names
    • FLJ39988 antibody
    • FLJ40036 antibody
    • FLJ40253 antibody
    • FLJ40587 antibody
    • FLJ40789 antibody
    • FLJ40834 antibody
    • G1m marker antibody
    • G2m marker antibody
    • G3m marker antibody
    • G4m marker antibody
    • HDC antibody
    • Heavy chain disease protein antibody
    • Human immunglobulin G antibody
    • Ig gamma 1 chain C region antibody
    • Ig gamma 2 chain C region antibody
    • Ig gamma 3 chain C region antibody
    • Ig gamma 4 chain C region antibody
    • IgG heavy chain locus antibody
    • IGHG 1 antibody
    • IGHG 2 antibody
    • IGHG 3 antibody
    • IGHG 4 antibody
    • IGHG1 antibody
    • IGHG2 antibody
    • IGHG3 antibody
    • IGHG4 antibody
    • Immunoglobulin Gm1 antibody
    • Immunoglobulin Gm3 antibody
    • Immunoglobulin Gm4 antibody
    • immunoglobulin heavy constant gamma 1 (G1m marker) antibody
    • Immunoglobulin heavy constant gamma 1 antibody
    • immunoglobulin heavy constant gamma 2 (G2m marker) antibody
    • Immunoglobulin heavy constant gamma 2 antibody
    • immunoglobulin heavy constant gamma 3 (G3m marker) antibody
    • Immunoglobulin heavy constant gamma 3 antibody
    • immunoglobulin heavy constant gamma 4 (G4m marker) antibody
    • Immunoglobulin heavy constant gamma 4 antibody
    • MGC117419 antibody
    • MGC45809 antibody
    see all

References

ab7497 has not yet been referenced specifically in any publications.

Publishing research using ab7497? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Question

Thanks for your response. I intend to employ ELISA method.
Thanks

Read More
Answer

Thank you for your reply.


I picked out three antibodies which I think are suitable for you. Please let me know if you need anything further, like matching secondary antibodies.

https://www.abcam.com/index.html?datasheet=1947 (or use the following: https://www.abcam.com/index.html?datasheet=1947).

https://www.abcam.com/index.html?datasheet=7400 (or use the following: https://www.abcam.com/index.html?datasheet=7400).

https://www.abcam.com/index.html?datasheet=7497 (or use the following: https://www.abcam.com/index.html?datasheet=7497).


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question

PRE-SALES ENQUIRY Does it recognise IgG4 and is it the heavy or light chain the antibody detects?

Read More
Answer

Thank you for your enquiry. Ab7497 is class-specific for human IgG and clone 2A11 is Fab-specific.

Read More

Question

What is the concentration?

Read More
Answer

Thank you for your enquiry. For both these antibodies the concentration varies from lot to lot; normally the concentration is from 1 to 10 mg/ml. We are currently out of stock for both of these; if you were to order one, I could obtain more information regarding the concentration. If you have any more questions, please contact us again.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up