Anti-Human IgG antibody [2A11] (ab7497)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-Human IgG antibody [2A11]
See all Human IgG primary antibodies -
Description
Mouse monoclonal [2A11] to Human IgG -
Host species
Mouse -
Specificity
Ab7497 specifically recognises Hinge region of Human IgG. No cross-reactivity with IgA,IgM,IgE. -
Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
These clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunised with IgG from human serum.
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General notes
Concentration varies from lot to lot and can be provided on request.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Ascites -
Purification notes
Purified from ascites. -
Clonality
Monoclonal -
Clone number
2A11 -
Myeloma
Sp2/0 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab7497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA | Use at an assay dependent dilution. |
Target
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Relevance
IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). -
Cellular localization
Cell Membrane and Secreted -
Database links
- Entrez Gene: 3500 Human
- Entrez Gene: 3501 Human
- Entrez Gene: 3502 Human
- Entrez Gene: 3503 Human
- Omim: 147100 Human
- Omim: 147110 Human
- Omim: 147120 Human
- Omim: 147130 Human
see all -
Alternative names
- FLJ39988 antibody
- FLJ40036 antibody
- FLJ40253 antibody
see all
References
This product has been referenced in:
- Marsalek L et al. Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris. Microb Cell Fact 18:119 (2019). Read more (PubMed: 31269943) »
- Fink M et al. Microbioreactor Cultivations of Fab-Producing Escherichia coli Reveal Genome-Integrated Systems as Suitable for Prospective Studies on Direct Fab Expression Effects. Biotechnol J 14:e1800637 (2019). Read more (PubMed: 31231932) »