Overview

  • Product name
    Anti-Human IgG antibody - ChIP Grade
    See all Human IgG primary antibodies
  • Description
    Rabbit polyclonal to Human IgG - ChIP Grade
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ChIP, Flow Cyt, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    IgG isolated from a pool of normal human sera.

  • Positive control
    • Tonsil

Properties

Applications

Our Abpromise guarantee covers the use of ab2410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent dilution. PubMed: 15252039Used as negative isotype control in PMID 15252039.
Flow Cyt Use at an assay dependent dilution.
IHC-P 1/200 - 1/500. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent dilution.

Target

  • Relevance
    IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
  • Cellular localization
    Cell Membrane and Secreted
  • Database links
  • Alternative names
    • FLJ39988 antibody
    • FLJ40036 antibody
    • FLJ40253 antibody
    • FLJ40587 antibody
    • FLJ40789 antibody
    • FLJ40834 antibody
    • G1m marker antibody
    • G2m marker antibody
    • G3m marker antibody
    • G4m marker antibody
    • HDC antibody
    • Heavy chain disease protein antibody
    • Human immunglobulin G antibody
    • Ig gamma 1 chain C region antibody
    • Ig gamma 2 chain C region antibody
    • Ig gamma 3 chain C region antibody
    • Ig gamma 4 chain C region antibody
    • IgG heavy chain locus antibody
    • IGHG 1 antibody
    • IGHG 2 antibody
    • IGHG 3 antibody
    • IGHG 4 antibody
    • IGHG1 antibody
    • IGHG2 antibody
    • IGHG3 antibody
    • IGHG4 antibody
    • Immunoglobulin Gm1 antibody
    • Immunoglobulin Gm3 antibody
    • Immunoglobulin Gm4 antibody
    • immunoglobulin heavy constant gamma 1 (G1m marker) antibody
    • Immunoglobulin heavy constant gamma 1 antibody
    • immunoglobulin heavy constant gamma 2 (G2m marker) antibody
    • Immunoglobulin heavy constant gamma 2 antibody
    • immunoglobulin heavy constant gamma 3 (G3m marker) antibody
    • Immunoglobulin heavy constant gamma 3 antibody
    • immunoglobulin heavy constant gamma 4 (G4m marker) antibody
    • Immunoglobulin heavy constant gamma 4 antibody
    • MGC117419 antibody
    • MGC45809 antibody
    see all

Images

  • ab2410 staining IgG in human tonsil sections

References

This product has been referenced in:
See all 20 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

We have not received any report of the this antibody being used for diagnosis of autoimmune diseases by IHC or other methods. It has been demonstrated to be capable of staining a sub-population of cells in tonsil sections, as shown on the datasheet, but detection of immune deposits of immunoglobulins may requirea more sensitive tecnique. If you do decide to try this antibody, please contact us if you have any difficulty.

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Answer

Thank you for your call today and for your questions.

Here are the products that we discussed:

1) Rabbit anti-human IgG (H&L) antibody: Ab6715

https://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Human-IgG-H-L-ab6715.html

2) Mouse anti-human IgG (H&L) antibody: Ab34834

https://www.abcam.com/Mouse-polyclonal-Secondary-Antibody-to-Human-IgG-H-L-ab34834.html

3) Anti-mouse IgG (H&L) antibody conjugated to AP: Ab7069

https://www.abcam.com/Mouse-IgG-secondary-antibody-H-L-Pre-Adsorbed-ab7069.html

This anti-mouse IgG antibody is pre-adsorbed against human IgG, so that it will not bind to the human IgG on the Western blot. It will only bind to the mouse primary antibody.

I hope this information will be useful, but please let me know if you have any further questions or if there is anything else that we can do for you. Have a nice day.

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Answer

Thank you for your reply.

I attached the MSDS to this email for your information.

I hope this ids helpful and wish you good luck with your research.

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Answer

Thank you for your inquiry.

The MSDS PDFsfor our products can be found on the online datasheets.

If there is not MSDS as in the case of ab2410, this indicates that the product does not contains hazardous substances or only contains so little that a MSDS is not necessary.

If you still require a MSDS for ab2410, I will be happy to ask the department to generate one for you (which would say, that it is not hazardous in these minute amounts).

I hope this information is helpful and wish you a good day.

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Answer

Vielen Dank für Ihre Nachrichten.

Leider sind die anti-human IgGs nicht die Kontrollen, die wir Ihnen als Isotypkontrolle für die IL9- und IL9R- Antikörper empfehlen würden. (Damit würden Sie sicher eine Färbung erhalten, die Sie so nicht beabsichtigen).

Als Isotypkontrolle können wir Ihnen ab27478 oder ab27472 (vorverdünnt=prediluted) empfehlen:

https://www.abcam.com/index.html?datasheet=27478 (or use the following: https://www.abcam.com/index.html?datasheet=27478).

https://www.abcam.com/index.html?datasheet=27472 (or use the following: https://www.abcam.com/index.html?datasheet=27472).

Es freut mich zu hören, dass Sie unser Angebot unsere Antikörper ab106287und ab111915an Gefrierschnitten zu testen, annehmen möchten.



Wie besprochen, können Sie den Gutscheincode zum Kauf jeweils eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test in IHC-Fr eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unsere Kundendienst mit dem Code und der ursprünglichen Bestellnummer.

Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung.

Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: https://www.abcam.com/collaborationdiscount

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Answer

Thank you for contacting us. I have heard back from the lab with the following information: This antibody was only tested on human tissues and not on any other species. Therefore, we do not know if it would crossreact with mouse or not. I am sorry to not have more helpful information for you in this regard. Please let me know if you would be looking into other proteins that might show low homology between human and mouse, and I can help you to find a suitable antibody. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us and I'm sorry to hear that you're having some trouble staining with ab2410. We haven't tested this antibody using monkey tissue, only human tissue (tonsil), so we haven't optimized the protocol for monkey. However, I've included some info about the protocol tested for you to try optimizing. Pretreatment enzymatic retrieval: We recommend histozyme treatment (Diagnostic Biosystem cat. #: K 046) for ab2410 for 5 minutes at room temperature. The antibody should be diluted even further if you're seeing background. Try 1/3000 - 1/5000 and dilute your secondary more too. The lab suggests incubating the primary for 30 mins at room temperature. Plus, what are you using for blocking? We'd recommend 5-10% serum from the species your secondary is raised in and incubating that for 1 hr at RT. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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