Key features and details
- Rabbit monoclonal [E20V] to Human IgG
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Human IgG antibody [E20V]
See all Human IgG primary antibodies
DescriptionRabbit monoclonal [E20V] to Human IgG
Tested applicationsSuitable for: IHC-Fr, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Human IgG (internal sequence). The exact sequence is proprietary.
Database link: P01857
EpitopeAntibody recognizes the epitope located between Val167 - Val185
- Human plasma cells in tonsillar tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 8.00
Preservative: 0.05% Sodium azide
Constituents: 2% BSA, 0.32% Tris HCl
Concentration information loading...
Purification notesThis immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. ab180555 is purified using a propriety technique and offers a completely post-translationally modified and properly glycosylated antibody. This offers increased stability.
Our Abpromise guarantee covers the use of ab180555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||1/100 - 1/300. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
RelevanceIgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
Cellular localizationCell Membrane and Secreted
- FLJ39988 antibody
- FLJ40036 antibody
- FLJ40253 antibody
ab180555 has not yet been referenced specifically in any publications.