Recombinant Anti-Human IgG antibody [EPR12700] (ab181236)
- Datasheet
- References (1)
- Protocols
Overview
-
Product name
Anti-Human IgG antibody [EPR12700]
See all Human IgG primary antibodies -
Description
Rabbit monoclonal [EPR12700] to Human IgG -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human Human IgG. Isolated from a pool of normal Human sera.
-
Positive control
- Human plasma, thymus, spleen and tonsil tissue lysates; Human IgG; Human spleen and colon tissues.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12700 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab181236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/250. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 52-58 kDa (predicted molecular weight: 36, 41 kDa). | |
| ELISA | Use at an assay dependent concentration. Please look at the product page for ab201842 for information on how this clone performs in an ELISA. |
Target
-
Relevance
IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). -
Cellular localization
Cell Membrane and Secreted -
Database links
- Entrez Gene: 3500 Human
- Entrez Gene: 3501 Human
- Entrez Gene: 3502 Human
- Entrez Gene: 3503 Human
- Omim: 147100 Human
- Omim: 147110 Human
- Omim: 147120 Human
- Omim: 147130 Human
see all -
Alternative names
- FLJ39988 antibody
- FLJ40036 antibody
- FLJ40253 antibody
see all
Images
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] (ab181236)](https://www.abcam.com/ps/products/181/ab181236/Images/ab181236-274508-anti-human-igg-antibody-epr12700-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] (ab181236)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling Human IgG with purified ab181236 at 1/100 dilution (1.3 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.
-
![Western blot - Anti-Human IgG antibody [EPR12700] (ab181236)](https://www.abcam.com/ps/products/181/ab181236/Images/ab181236-274506-anti-human-igg-antibody-epr12700-western-blot.jpg)
Western blot - Anti-Human IgG antibody [EPR12700] (ab181236)Anti-Human IgG antibody [EPR12700] (ab181236) at 1/10000 dilution (purified) + Human tonsil lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 36, 41 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] (ab181236)](https://www.abcam.com/ps/products/181/ab181236/Images/ab181236-214514-anti-human-igg-antibody-epr12700-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] (ab181236)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Human IgG with unpurified ab181236 at 1/250 dilution, dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
-
![Western blot - Anti-Human IgG antibody [EPR12700] (ab181236)](https://www.abcam.com/ps/products/181/ab181236/Images/ab181236-274505-anti-human-igg-antibody-epr12700-western-blot.jpg)
Western blot - Anti-Human IgG antibody [EPR12700] (ab181236)Anti-Human IgG antibody [EPR12700] (ab181236) at 1/2000 dilution (purified) + Human spleen lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 36, 41 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
-
![Western blot - Anti-Human IgG antibody [EPR12700] (ab181236)](https://www.abcam.com/ps/products/181/ab181236/Images/ab181236-214511-anti-human-igg-antibody-epr12700-western-blot.jpg)
Western blot - Anti-Human IgG antibody [EPR12700] (ab181236)All lanes : Anti-Human IgG antibody [EPR12700] (ab181236) at 1/10000 dilution (unpurified)
Lane 1 : Human plasma lysate
Lane 2 : Human thymus lysate
Lane 3 : Human tonsil lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 36, 41 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] (ab181236)](https://www.abcam.com/ps/products/181/ab181236/Images/ab181236-214513-anti-human-igg-antibody-epr12700-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] (ab181236)Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Human IgG with unpurified ab181236 at 1/250 dilution, dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
-
![Western blot - Anti-Human IgG antibody [EPR12700] (ab181236)](https://www.abcam.com/ps/products/181/ab181236/Images/ab181236-214512-anti-human-igg-antibody-epr12700-western-blot.jpg)
Western blot - Anti-Human IgG antibody [EPR12700] (ab181236)All lanes : Anti-Human IgG antibody [EPR12700] (ab181236) at 1/2000 dilution (unpurified)
Lane 1 : Human IgG
Lane 2 : Human IgM
Lane 3 : Human IgA
Lane 4 : Human IgD
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36, 41 kDa
References
This product has been referenced in:
- Vergauwen G et al. Confounding factors of ultrafiltration and protein analysis in extracellular vesicle research. Sci Rep 7:2704 (2017). Read more (PubMed: 28577337) »
