Overview

  • Product name

    Anti-Human IgG antibody [EPR12700]
    See all Human IgG primary antibodies
  • Description

    Rabbit monoclonal [EPR12700] to Human IgG
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Human IgG. Isolated from a pool of normal Human sera.

  • Positive control

    • Human plasma, thymus, spleen and tonsil tissue lysates; Human IgG; Human spleen and colon tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12700
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250.

See IHC antigen retrieval protocols.

WB 1/1000 - 1/10000. Detects a band of approximately 52-58 kDa (predicted molecular weight: 36, 41 kDa).
ELISA Use at an assay dependent concentration.

Please look at the product page for ab201842 for information on how this clone performs in an ELISA.

Target

  • Relevance

    IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
  • Cellular localization

    Cell Membrane and Secreted
  • Database links

  • Alternative names

    • FLJ39988 antibody
    • FLJ40036 antibody
    • FLJ40253 antibody
    • FLJ40587 antibody
    • FLJ40789 antibody
    • FLJ40834 antibody
    • G1m marker antibody
    • G2m marker antibody
    • G3m marker antibody
    • G4m marker antibody
    • HDC antibody
    • Heavy chain disease protein antibody
    • Human immunglobulin G antibody
    • Ig gamma 1 chain C region antibody
    • Ig gamma 2 chain C region antibody
    • Ig gamma 3 chain C region antibody
    • Ig gamma 4 chain C region antibody
    • IgG heavy chain locus antibody
    • IGHG 1 antibody
    • IGHG 2 antibody
    • IGHG 3 antibody
    • IGHG 4 antibody
    • IGHG1 antibody
    • IGHG2 antibody
    • IGHG3 antibody
    • IGHG4 antibody
    • Immunoglobulin Gm1 antibody
    • Immunoglobulin Gm3 antibody
    • Immunoglobulin Gm4 antibody
    • immunoglobulin heavy constant gamma 1 (G1m marker) antibody
    • Immunoglobulin heavy constant gamma 1 antibody
    • immunoglobulin heavy constant gamma 2 (G2m marker) antibody
    • Immunoglobulin heavy constant gamma 2 antibody
    • immunoglobulin heavy constant gamma 3 (G3m marker) antibody
    • Immunoglobulin heavy constant gamma 3 antibody
    • immunoglobulin heavy constant gamma 4 (G4m marker) antibody
    • Immunoglobulin heavy constant gamma 4 antibody
    • MGC117419 antibody
    • MGC45809 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling Human IgG with purified ab181236 at 1/100 dilution (1.3 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

  • Anti-Human IgG antibody [EPR12700] (ab181236) at 1/10000 dilution (purified) + Human tonsil lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 36, 41 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Human IgG with unpurified ab181236 at 1/250 dilution, dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Anti-Human IgG antibody [EPR12700] (ab181236) at 1/2000 dilution (purified) + Human spleen lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 36, 41 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Anti-Human IgG antibody [EPR12700] (ab181236) at 1/10000 dilution (unpurified)

    Lane 1 : Human plasma lysate
    Lane 2 : Human thymus lysate
    Lane 3 : Human tonsil lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 36, 41 kDa

  • Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Human IgG with unpurified ab181236 at 1/250 dilution, dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • All lanes : Anti-Human IgG antibody [EPR12700] (ab181236) at 1/2000 dilution (unpurified)

    Lane 1 : Human IgG
    Lane 2 : Human IgM
    Lane 3 : Human IgA
    Lane 4 : Human IgD

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36, 41 kDa

References

This product has been referenced in:

  • Vergauwen G  et al. Confounding factors of ultrafiltration and protein analysis in extracellular vesicle research. Sci Rep 7:2704 (2017). Read more (PubMed: 28577337) »
See 1 Publication for this product

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