RecombinantRabMAb

Recombinant Anti-Human IgG antibody [EPR4421] (ab109489)

Reviews (3)Q&A (6)References (64)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4421] to Human IgG
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Human IgG antibody [EPR4421]
    See all Human IgG primary antibodies

  • Description

    Rabbit monoclonal [EPR4421] to Human IgG

  • Host species

    Rabbit

  • Specificity

    ab109489 recognizes all four Human IgG isotypes (IgG1, IgG2, IgG3, and IgG4).

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Human IgG aa 150-250 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • WB: Human IgG2, IgG1, IgG3, and IgG4 recombinant proteins, human fetal thymus, fetal tonsil and fetal spleen tissue lysates. IHC-P: Human tonsil and stomach tissues.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR4421

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 52-58 kDa.
IHC-P 1/300 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Target

  • Relevance

    IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

  • Cellular localization

    Cell Membrane and Secreted

  • Database links

    see all

  • Alternative names

    • FLJ39988 antibody
    • FLJ40036 antibody
    • FLJ40253 antibody
    • FLJ40587 antibody
    • FLJ40789 antibody
    • FLJ40834 antibody
    • G1m marker antibody
    • G2m marker antibody
    • G3m marker antibody
    • G4m marker antibody
    • HDC antibody
    • Heavy chain disease protein antibody
    • Human immunglobulin G antibody
    • Ig gamma 1 chain C region antibody
    • Ig gamma 2 chain C region antibody
    • Ig gamma 3 chain C region antibody
    • Ig gamma 4 chain C region antibody
    • IgG heavy chain locus antibody
    • IGHG 1 antibody
    • IGHG 2 antibody
    • IGHG 3 antibody
    • IGHG 4 antibody
    • IGHG1 antibody
    • IGHG2 antibody
    • IGHG3 antibody
    • IGHG4 antibody
    • Immunoglobulin Gm1 antibody
    • Immunoglobulin Gm3 antibody
    • Immunoglobulin Gm4 antibody
    • immunoglobulin heavy constant gamma 1 (G1m marker) antibody
    • Immunoglobulin heavy constant gamma 1 antibody
    • immunoglobulin heavy constant gamma 2 (G2m marker) antibody
    • Immunoglobulin heavy constant gamma 2 antibody
    • immunoglobulin heavy constant gamma 3 (G3m marker) antibody
    • Immunoglobulin heavy constant gamma 3 antibody
    • immunoglobulin heavy constant gamma 4 (G4m marker) antibody
    • Immunoglobulin heavy constant gamma 4 antibody
    • MGC117419 antibody
    • MGC45809 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (ab109489)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (ab109489)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling Human IgG with purified ab109489 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)
    Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)
    All lanes : Anti-Human IgG antibody [EPR4421] (ab109489) at 1/20000 dilution (purified)

    Lane 1 : Human fetal thymus tissue lysate
    Lane 2 : Human fetal tonsil tissue lysate
    Lane 3 : Human fetal spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)
    Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)
    All lanes : Anti-Human IgG antibody [EPR4421] (ab109489) at 1/1000 dilution (unpurified)

    Lane 1 : IgG2 recombinant protein (10 ng protein loaded)
    Lane 2 : IgG1 recombinant protein (10 ng protein loaded)
    Lane 3 : IgG3 recombinant protein (10 ng protein loaded)
    Lane 4 : IgG4 recombinant protein (10 ng protein loaded)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (ab109489)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (ab109489)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling IgG with unpurified ab109489 at a dilution of 1/500.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)
    Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)
    Anti-Human IgG antibody [EPR4421] (ab109489) at 1/1000 dilution (purified) + IgG3 recombinant protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)
    Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)
    All lanes : Anti-Human IgG antibody [EPR4421] (ab109489) at 1/5000 dilution (purified)

    Lane 1 : IgG1 recombinant protein
    Lane 2 : IgG2 recombinant protein
    Lane 3 : IgG4 recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (ab109489)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (ab109489)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling IgG with unpurified ab109489 at a dilution of 1/500.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Human IgG antibody [EPR4421] (ab109489)
    Anti-Human IgG antibody [EPR4421] (ab109489)

References (64)

Publishing research using ab109489? Please let us know so that we can cite the reference in this datasheet.

ab109489 has been referenced in 64 publications.

  • Gong D  et al. Silencing Long Non-coding RNA LINC01224 Inhibits Hepatocellular Carcinoma Progression via MicroRNA-330-5p-Induced Inhibition of CHEK1. Mol Ther Nucleic Acids 19:482-497 (2020). PubMed: 31902747
  • Wang D  et al. Soluble CD146, a cerebrospinal fluid marker for neuroinflammation, promotes blood-brain barrier dysfunction. Theranostics 10:231-246 (2020). PubMed: 31903117
  • Fan L  et al. LINC00908 negatively regulates microRNA-483-5p to increase TSPYL5 expression and inhibit the development of prostate cancer. Cancer Cell Int 20:10 (2020). PubMed: 31938018
  • Li B  et al. The MSC-Derived Exosomal lncRNA H19 Promotes Wound Healing in Diabetic Foot Ulcers by Upregulating PTEN via MicroRNA-152-3p. Mol Ther Nucleic Acids 19:814-826 (2020). PubMed: 31958697
  • Zhu S  et al. Long non-coding RNA HOXA11-AS upregulates Cyclin D2 to inhibit apoptosis and promote cell cycle progression in nephroblastoma by recruiting forkhead box P2. Am J Cancer Res 10:284-298 (2020). PubMed: 32064168
View all Publications for this product

Customer reviews and Q&As

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1-9 of 9 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Human IgG antibody [EPR4421]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Breast cancer cell line)
Permeabilization
No
Specification
Breast cancer cell line
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jul 05 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Human IgG antibody [EPR4421]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast carcinoma and Breast cell lines)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6 and EDTA pH8.5
Permeabilization
No
Specification
Breast carcinoma and Breast cell lines
Blocking step
(agent) for 10 minute(s) · Concentration: 50% · Temperature: 24°C
Fixative
Formaldehyde
Read More

Mr. Malcolm Lim

Verified customer

Submitted Jun 27 2017

Flow Cytometry abreview for Anti-Human IgG antibody [EPR4421]

 Average
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (PBMC)
Permeabilization
Yes - 4x Cytofix, 45 min, 4 ¯C
Gating Strategy
CD19 positive live cells
Specification
PBMC
Preparation
Cell harvesting/tissue preparation method: PBMC from whole blood by density gradient (lymphoprep, Fresenius)
Sample buffer: D-PBS+5 % FCS + 2 mM EDTA
Read More

Clara Lettl

Verified customer

Submitted Jan 18 2017

Question

How was it determined that this antibody does not cross react with mouse? I'd like to use it in WB and IHC-P to detect human IgG specifically in a human sample xenografted into mouse tissue.

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Answer

We tested ab109489 on mouse kidney, spleen, heart, brain, serum and thymus lysates in WB and did not see any bands at all (results attached). For this reason, we do not recommend the antibody for use with mouse nor do we expect cross-reactivity.

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Question

Inquiry: Dear ma'm/sir I would like to know whether the following product, is HRP-conjugated or not. From the picture in your site, I can see it has been used in westernblot films. ab109489

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Answer

Thank you for contacting us.

ab109489 is not HRP-conjugated and you are correct, it is guaranteed to work in western blot.

If you wanted an anti-human IgG antibody conjugated to HRP, we have several secondary antibodies available in our catalogue.

ab99765 (https://www.abcam.com/ab99765) is a Mouse monoclonal [H2] Secondary Antibody to Human
IgG - Fc HRP conjugated.

ab102410 (https://www.abcam.com/ab102410) is a Donkey polyclonal Secondary Antibody to Human IgG - H&L (HRP).

If these secondary antibodies are the sorts of antibodies that you would be interested in, I would be happy to help you chose the most suitable antibody for your research.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Question

Ah, that was actually also one that caught my eye. (antigen is located in the CH2 doamin of IgG , see attachments). Is it possible to receive a “test vial”?

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Answer

Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our AbTrial program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information. Or you can find more information about this program at the following link:

www.abcam.com/abtrial

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I have compared the immunogen sequence similarity between human and Cynomolgus and have found 72.3% homology. We suggest that less than 85% homology is a good indicator that the antibody will not cross react however, we cannot guarentee this.

At the moment we are having a promotion on rabbit monoclonals such as ab109489. If you buy any primary antibody you get a free Rabbit monoclonal antibody if you add the following code to your purchase order: RABMAB-XBSMG

This might be something that may interest you as it would allow you to try ab109489 for free.

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

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Question

Thank you for the prompt reply. The application would be in an immunoassay the methodology can be ECLIA, ELISA, SPR etc. The preference would be recognition of all IgG subclasses, however, the antibody should have been demonstrated to not react with (all) Cynomolgus IgG (subclasses).

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Answer

Thank you for your inquiry.

Unfortunately, we do not have any antibodies against huamn IgG which has been demonstrated to not react with (all) Cynomolgus IgG available in our catalog.

We have one antibody ab109489 for which we have the immunogen for (https://www.abcam.com/ab109489). I have tried to do a sequence alignment to find out whether this antibody is likely to recognise Cynomolgus IgG however, I could not find the amino acid sequence for Cynomolgus IgG. If you have it, I would be happy to execute a sequence alignment for you. Alternatively,I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are:
www.biocompare.com
http://www.biocompare.com/ProductCategories/2045/Antibodies.html

We are happy to be able to offer custom antibody services through Epitomics, an Abcam company. For more information about these services visit:

http://www.epitomics.com/services/
email: service@epitomics.com

Alternatively, please contact Li Li at Epitomics:

li.li@epitomics.com

I hope this information is nevertheless helpful to you. Please do not hesitate to contact me if you have any further questions in this regard.

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Question

Phone call enquiring about the antibodies ab1939, ab109489 and ab134083

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Answer

Thank you for contacting us today.

I am sorry for the delay in getting back to you. As discussed over the phone, as you intend to use the antibodies ab1939, ab109489 and ab134083 in Western blotting and IHC, we would be able to offer you a testing discount for ab1939 for which we have not tested the antibody in IHC of paraffin embedded sections.

This offer means that if you purchase ab1939 as normal, test the antibody with your human samples in IHC and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price of ab1939 off any product). More information on this offer can be found here:

www.abcam.com/collaborationdiscount

Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab1939.



I am still looking into which positive control tissue to recommend with ab1939. Once I have this information I will get back to you.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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Question

Cette numérotation aa est bien à partir du N-terminal sur la chaine lourde d'une IgG ? Si oui cette région serait alors dans le domaine CH1. S'il vous plaît pouvez-vous me confirmer cela ?  

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Answer

L'immunogène correspond à la séquence aa 155-185 de la chaine lourde gamma 1 référence UniProt P01857 (http://www.uniprot.org/uniprot/P01857). Les acides aminés 155 à 185 correspondent au domaine CH2. J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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