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RecombinantRabMAb

Recombinant Anti-Human IgG antibody [EPR4421] (ab109489)

Reviews (3)Q&A (6)References (174)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (ab109489)
  • Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)
  • Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (ab109489)
  • Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)
  • Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (ab109489)
  • Anti-Human IgG antibody [EPR4421] (ab109489)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4421] to Human IgG
  • Suitable for: WB, IHC-P
  • Reacts with: Human, Recombinant fragment

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Human IgG antibody [EPR4421]
    See all Human IgG primary antibodies

  • Description

    Rabbit monoclonal [EPR4421] to Human IgG

  • Host species

    Rabbit

  • Specificity

    ab109489 recognizes all four Human IgG isotypes (IgG1, IgG2, IgG3, and IgG4).

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human, Recombinant fragment

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human IgG2, IgG1, IgG3, and IgG4 recombinant proteins, human fetal thymus, fetal tonsil and fetal spleen tissue lysates. IHC-P: Human tonsil and stomach tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR4421

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 52-58 kDa.
IHC-P (1)
1/300 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 52-58 kDa.
IHC-P
1/300 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Target

  • Relevance

    IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

  • Cellular localization

    Cell Membrane and Secreted

  • Database links

    see all

  • Alternative names

    • FLJ39988 antibody
    • FLJ40036 antibody
    • FLJ40253 antibody
    • FLJ40587 antibody
    • FLJ40789 antibody
    • FLJ40834 antibody
    • G1m marker antibody
    • G2m marker antibody
    • G3m marker antibody
    • G4m marker antibody
    • HDC antibody
    • Heavy chain disease protein antibody
    • Human immunglobulin G antibody
    • Ig gamma 1 chain C region antibody
    • Ig gamma 2 chain C region antibody
    • Ig gamma 3 chain C region antibody
    • Ig gamma 4 chain C region antibody
    • IgG heavy chain locus antibody
    • IGHG 1 antibody
    • IGHG 2 antibody
    • IGHG 3 antibody
    • IGHG 4 antibody
    • IGHG1 antibody
    • IGHG2 antibody
    • IGHG3 antibody
    • IGHG4 antibody
    • Immunoglobulin Gm1 antibody
    • Immunoglobulin Gm3 antibody
    • Immunoglobulin Gm4 antibody
    • immunoglobulin heavy constant gamma 1 (G1m marker) antibody
    • Immunoglobulin heavy constant gamma 1 antibody
    • immunoglobulin heavy constant gamma 2 (G2m marker) antibody
    • Immunoglobulin heavy constant gamma 2 antibody
    • immunoglobulin heavy constant gamma 3 (G3m marker) antibody
    • Immunoglobulin heavy constant gamma 3 antibody
    • immunoglobulin heavy constant gamma 4 (G4m marker) antibody
    • Immunoglobulin heavy constant gamma 4 antibody
    • MGC117419 antibody
    • MGC45809 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (ab109489)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (ab109489)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling Human IgG with purified ab109489 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)
    Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)
    All lanes : Anti-Human IgG antibody [EPR4421] (ab109489) at 1/20000 dilution (purified)

    Lane 1 : Human fetal thymus tissue lysate
    Lane 2 : Human fetal tonsil tissue lysate
    Lane 3 : Human fetal spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)
    Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)
    All lanes : Anti-Human IgG antibody [EPR4421] (ab109489) at 1/1000 dilution (unpurified)

    Lane 1 : IgG2 recombinant protein (10 ng protein loaded)
    Lane 2 : IgG1 recombinant protein (10 ng protein loaded)
    Lane 3 : IgG3 recombinant protein (10 ng protein loaded)
    Lane 4 : IgG4 recombinant protein (10 ng protein loaded)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (ab109489)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (ab109489)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling IgG with unpurified ab109489 at a dilution of 1/500.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)
    Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)
    Anti-Human IgG antibody [EPR4421] (ab109489) at 1/1000 dilution (purified) + IgG3 recombinant protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)
    Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)
    All lanes : Anti-Human IgG antibody [EPR4421] (ab109489) at 1/5000 dilution (purified)

    Lane 1 : IgG1 recombinant protein
    Lane 2 : IgG2 recombinant protein
    Lane 3 : IgG4 recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (ab109489)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (ab109489)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling IgG with unpurified ab109489 at a dilution of 1/500.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Human IgG antibody [EPR4421] (ab109489)
    Anti-Human IgG antibody [EPR4421] (ab109489)

References (174)

Publishing research using ab109489? Please let us know so that we can cite the reference in this datasheet.

ab109489 has been referenced in 174 publications.

  • Ye M  et al. Oncogenic Role of Long Noncoding RNAMALAT1 in Thyroid Cancer Progression through Regulation of the miR-204/IGF2BP2/m6A-MYC Signaling. Mol Ther Nucleic Acids 23:1-12 (2021). PubMed: 33312756
  • Wang S  et al. Transfer of microRNA-25 by colorectal cancer cell-derived extracellular vesicles facilitates colorectal cancer development and metastasis. Mol Ther Nucleic Acids 23:552-564 (2021). PubMed: 33510943
  • Li M  et al. Deep dive on the proteome of salivary extracellular vesicles: comparison between ultracentrifugation and polymer-based precipitation isolation. Anal Bioanal Chem 413:365-375 (2021). PubMed: 33159572
  • Song F  et al. Long non-coding RNA MIAT promotes the proliferation and invasion of laryngeal squamous cell carcinoma cells by sponging microRNA-613. Exp Ther Med 21:232 (2021). PubMed: 33603840
  • Mao Z  et al. CircCDR1as Suppresses Bone Microvascular Endothelial Cell Activity and Angiogenesis Through Targeting miR-135b/ FIH-1 Axis. Orthop Surg 13:573-582 (2021). PubMed: 33619902
View all Publications for this product

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Human IgG antibody [EPR4421]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast carcinoma and Breast cell lines)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6 and EDTA pH8.5
Permeabilization
No
Specification
Breast carcinoma and Breast cell lines
Blocking step
(agent) for 10 minute(s) · Concentration: 50% · Temperature: 24°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Malcolm Lim

Verified customer

Submitted Jun 27 2017

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