Recombinant
RabMAb

Recombinant Anti-Human IgG antibody [EPR4421] (ab109489)

Rabbit recombinant monoclonal Human IgG antibody [EPR4421]. Validated in WB, IHC and tested in Human. Cited in 16 publication(s). Independently reviewed in 3 review(s).

Overview

  • Product name

    Anti-Human IgG antibody [EPR4421]
    See all Human IgG primary antibodies
  • Description

    Rabbit monoclonal [EPR4421] to Human IgG
  • Host species

    Rabbit
  • Specificity

    ab109489 recognizes all four Human IgG isotypes (IgG1, IgG2, IgG3, and IgG4).
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Human IgG aa 150-250 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • WB: Human IgG2, IgG1, IgG3, and IgG4 recombinant proteins, human fetal thymus, fetal tonsil and fetal spleen tissue lysates. IHC-P: Human tonsil and stomach tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4421
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 52-58 kDa.
IHC-P 1/300 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Target

  • Relevance

    IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
  • Cellular localization

    Cell Membrane and Secreted
  • Database links

  • Alternative names

    • FLJ39988 antibody
    • FLJ40036 antibody
    • FLJ40253 antibody
    • FLJ40587 antibody
    • FLJ40789 antibody
    • FLJ40834 antibody
    • G1m marker antibody
    • G2m marker antibody
    • G3m marker antibody
    • G4m marker antibody
    • HDC antibody
    • Heavy chain disease protein antibody
    • Human immunglobulin G antibody
    • Ig gamma 1 chain C region antibody
    • Ig gamma 2 chain C region antibody
    • Ig gamma 3 chain C region antibody
    • Ig gamma 4 chain C region antibody
    • IgG heavy chain locus antibody
    • IGHG 1 antibody
    • IGHG 2 antibody
    • IGHG 3 antibody
    • IGHG 4 antibody
    • IGHG1 antibody
    • IGHG2 antibody
    • IGHG3 antibody
    • IGHG4 antibody
    • Immunoglobulin Gm1 antibody
    • Immunoglobulin Gm3 antibody
    • Immunoglobulin Gm4 antibody
    • immunoglobulin heavy constant gamma 1 (G1m marker) antibody
    • Immunoglobulin heavy constant gamma 1 antibody
    • immunoglobulin heavy constant gamma 2 (G2m marker) antibody
    • Immunoglobulin heavy constant gamma 2 antibody
    • immunoglobulin heavy constant gamma 3 (G3m marker) antibody
    • Immunoglobulin heavy constant gamma 3 antibody
    • immunoglobulin heavy constant gamma 4 (G4m marker) antibody
    • Immunoglobulin heavy constant gamma 4 antibody
    • MGC117419 antibody
    • MGC45809 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling Human IgG with purified ab109489 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • All lanes : Anti-Human IgG antibody [EPR4421] (ab109489) at 1/20000 dilution (purified)

    Lane 1 : Human fetal thymus tissue lysate
    Lane 2 : Human fetal tonsil tissue lysate
    Lane 3 : Human fetal spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Human IgG antibody [EPR4421] (ab109489) at 1/1000 dilution (unpurified)

    Lane 1 : IgG2 recombinant protein (10 ng protein loaded)
    Lane 2 : IgG1 recombinant protein (10 ng protein loaded)
    Lane 3 : IgG3 recombinant protein (10 ng protein loaded)
    Lane 4 : IgG4 recombinant protein (10 ng protein loaded)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling IgG with unpurified ab109489 at a dilution of 1/500.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Human IgG antibody [EPR4421] (ab109489) at 1/1000 dilution (purified) + IgG3 recombinant protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Human IgG antibody [EPR4421] (ab109489) at 1/5000 dilution (purified)

    Lane 1 : IgG1 recombinant protein
    Lane 2 : IgG2 recombinant protein
    Lane 3 : IgG4 recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling IgG with unpurified ab109489 at a dilution of 1/500.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Liang TS  et al. MicroRNA-506 inhibits tumor growth and metastasis in nasopharyngeal carcinoma through the inactivation of the Wnt/ß-catenin signaling pathway by down-regulating LHX2. J Exp Clin Cancer Res 38:97 (2019). Read more (PubMed: 30791932) »
  • Xiu DH  et al. Long non-coding RNA LINC00968 attenuates drug resistance of breast cancer cells through inhibiting the Wnt2/ß-catenin signaling pathway by regulating WNT2. J Exp Clin Cancer Res 38:94 (2019). Read more (PubMed: 30791958) »
See all 31 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Breast cancer cell line)
Permeabilization
No
Specification
Breast cancer cell line
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 05 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast carcinoma and Breast cell lines)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6 and EDTA pH8.5
Permeabilization
No
Specification
Breast carcinoma and Breast cell lines
Blocking step
(agent) for 10 minute(s) · Concentration: 50% · Temperature: 24°C
Fixative
Formaldehyde

Mr. Malcolm Lim

Verified customer

Submitted Jun 27 2017

Application
Flow Cytometry
Sample
Human Cell (PBMC)
Permeabilization
Yes - 4x Cytofix, 45 min, 4 ¯C
Gating Strategy
CD19 positive live cells
Specification
PBMC
Preparation
Cell harvesting/tissue preparation method: PBMC from whole blood by density gradient (lymphoprep, Fresenius)
Sample buffer: D-PBS+5 % FCS + 2 mM EDTA

Clara Lettl

Verified customer

Submitted Jan 18 2017

Answer

We tested ab109489 on mouse kidney, spleen, heart, brain, serum and thymus lysates in WB and did not see any bands at all (results attached). For this reason, we do not recommend the antibody for use with mouse nor do we expect cross-reactivity.

Read More

Answer

Thank you for contacting us.

ab109489 is not HRP-conjugated and you are correct, it is guaranteed to work in western blot.

If you wanted an anti-human IgG antibody conjugated to HRP, we have several secondary antibodies available in our catalogue.

ab99765 (https://www.abcam.com/ab99765) is a Mouse monoclonal [H2] Secondary Antibody to Human
IgG - Fc HRP conjugated.

ab102410 (https://www.abcam.com/ab102410) is a Donkey polyclonal Secondary Antibody to Human IgG - H&L (HRP).

If these secondary antibodies are the sorts of antibodies that you would be interested in, I would be happy to help you chose the most suitable antibody for your research.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our AbTrial program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information. Or you can find more information about this program at the following link:

www.abcam.com/abtrial

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I have compared the immunogen sequence similarity between human and Cynomolgus and have found 72.3% homology. We suggest that less than 85% homology is a good indicator that the antibody will not cross react however, we cannot guarentee this.

At the moment we are having a promotion on rabbit monoclonals such as ab109489. If you buy any primary antibody you get a free Rabbit monoclonal antibody if you add the following code to your purchase order: RABMAB-XBSMG

This might be something that may interest you as it would allow you to try ab109489 for free.

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Read More

Answer

Thank you for your inquiry.

Unfortunately, we do not have any antibodies against huamn IgG which has been demonstrated to not react with (all) Cynomolgus IgG available in our catalog.

We have one antibody ab109489 for which we have the immunogen for (https://www.abcam.com/ab109489). I have tried to do a sequence alignment to find out whether this antibody is likely to recognise Cynomolgus IgG however, I could not find the amino acid sequence for Cynomolgus IgG. If you have it, I would be happy to execute a sequence alignment for you. Alternatively,I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are:
www.biocompare.com
http://www.biocompare.com/ProductCategories/2045/Antibodies.html

We are happy to be able to offer custom antibody services through Epitomics, an Abcam company. For more information about these services visit:

http://www.epitomics.com/services/
email: service@epitomics.com

Alternatively, please contact Li Li at Epitomics:

li.li@epitomics.com

I hope this information is nevertheless helpful to you. Please do not hesitate to contact me if you have any further questions in this regard.

Read More

Question
Answer

Thank you for contacting us today.

I am sorry for the delay in getting back to you. As discussed over the phone, as you intend to use the antibodies ab1939, ab109489 and ab134083 in Western blotting and IHC, we would be able to offer you a testing discount for ab1939 for which we have not tested the antibody in IHC of paraffin embedded sections.

This offer means that if you purchase ab1939 as normal, test the antibody with your human samples in IHC and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price of ab1939 off any product). More information on this offer can be found here:

www.abcam.com/collaborationdiscount

Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab1939.



I am still looking into which positive control tissue to recommend with ab1939. Once I have this information I will get back to you.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Read More

Answer

L'immunogène correspond à la séquence aa 155-185 de la chaine lourde gamma 1 référence UniProt P01857 (http://www.uniprot.org/uniprot/P01857). Les acides aminés 155 à 185 correspondent au domaine CH2. J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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