Recombinant Anti-Human IgG antibody [EPR4421] (ab109489)

We tested ab109489 on mouse kidney, spleen, heart, brain, serum and thymus lysates in WB and did not see any bands at all (results attached). For this reason, we do not recommend the antibody for use with mouse nor do we expect cross-reactivity.

Thank you for contacting us.

ab109489 is not HRP-conjugated and you are correct, it is guaranteed to work in western blot.

If you wanted an anti-human IgG antibody conjugated to HRP, we have several secondary antibodies available in our catalogue.

ab99765 (https://www.abcam.com/ab99765) is a Mouse monoclonal [H2] Secondary Antibody to Human
IgG - Fc HRP conjugated.

ab102410 (https://www.abcam.com/ab102410) is a Donkey polyclonal Secondary Antibody to Human IgG - H&L (HRP).

If these secondary antibodies are the sorts of antibodies that you would be interested in, I would be happy to help you chose the most suitable antibody for your research.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our AbTrial program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information. Or you can find more information about this program at the following link:

www.abcam.com/abtrial

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I have compared the immunogen sequence similarity between human and Cynomolgus and have found 72.3% homology. We suggest that less than 85% homology is a good indicator that the antibody will not cross react however, we cannot guarentee this.

At the moment we are having a promotion on rabbit monoclonals such as ab109489. If you buy any primary antibody you get a free Rabbit monoclonal antibody if you add the following code to your purchase order: RABMAB-XBSMG

This might be something that may interest you as it would allow you to try ab109489 for free.

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Thank you for your inquiry.

Unfortunately, we do not have any antibodies against huamn IgG which has been demonstrated to not react with (all) Cynomolgus IgG available in our catalog.

We have one antibody ab109489 for which we have the immunogen for (https://www.abcam.com/ab109489). I have tried to do a sequence alignment to find out whether this antibody is likely to recognise Cynomolgus IgG however, I could not find the amino acid sequence for Cynomolgus IgG. If you have it, I would be happy to execute a sequence alignment for you. Alternatively,I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are:
www.biocompare.com
http://www.biocompare.com/ProductCategories/2045/Antibodies.html

We are happy to be able to offer custom antibody services through Epitomics, an Abcam company. For more information about these services visit:

http://www.epitomics.com/services/
email: service@epitomics.com

Alternatively, please contact Li Li at Epitomics:

li.li@epitomics.com

I hope this information is nevertheless helpful to you. Please do not hesitate to contact me if you have any further questions in this regard.

Thank you for contacting us today.

I am sorry for the delay in getting back to you. As discussed over the phone, as you intend to use the antibodies ab1939, ab109489 and ab134083 in Western blotting and IHC, we would be able to offer you a testing discount for ab1939 for which we have not tested the antibody in IHC of paraffin embedded sections.

This offer means that if you purchase ab1939 as normal, test the antibody with your human samples in IHC and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price of ab1939 off any product). More information on this offer can be found here:

www.abcam.com/collaborationdiscount

Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab1939.



I am still looking into which positive control tissue to recommend with ab1939. Once I have this information I will get back to you.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

L'immunogène correspond à la séquence aa 155-185 de la chaine lourde gamma 1 référence UniProt P01857 (http://www.uniprot.org/uniprot/P01857). Les acides aminés 155 à 185 correspondent au domaine CH2. J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.