Key features and details
- Goat polyclonal to Human IgG (FITC)
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Product nameAnti-Human IgG antibody (FITC)
See all Human IgG primary antibodies
DescriptionGoat polyclonal to Human IgG (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
SpecificityLow background fluorescence of normal tissue in contrast to cases of autoimmune disease, deposited immunoglobulin IgG. No cross reactivity with albumin fractions of human serum. No cross reactivity with albumin fractions of animal species.
Tested applicationsSuitable for: IHC-Fr, Flow Cytmore details
Species reactivityReacts with: Human
The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel filtation and ion exchange chromatography to clear unbound conjugate. F/P ratio: 4-6 mol Fluorescein per 1 mol of goat IgG.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.50
Constituents: 50% Glycerol, 0.58% Sodium chloride, 0.164% Sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesFrom the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose bound human IgA and IgM. Specific antibodies were absorbed by incubation with Sepharose bound human IgG. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgG a minimum of 65% protein bound.
Our Abpromise guarantee covers the use of ab81051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab37394 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceIgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
Cellular localizationCell Membrane and Secreted
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ab81051 has been referenced in 1 publication.
- Gramlich OW et al. Enhanced insight into the autoimmune component of glaucoma: IgG autoantibody accumulation and pro-inflammatory conditions in human glaucomatous retina. PLoS One 8:e57557 (2013). ICC/IF ; Human . PubMed: 23451242