Overview

  • Product name
    Anti-Human IgG antibody [IG266]
    See all Human IgG primary antibodies
  • Description
    Mouse monoclonal [IG266] to Human IgG
  • Host species
    Mouse
  • Specificity
    ab200699 recognizes a protein of 75 kDa, identified as gamma heavy chain of Human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes.
  • Tested applications
    Suitable for: IHC-P, WB, IHC-Fr, ICC/IF, Flow Cyt, IP, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human IgG. P01857, P01859, P01860, P01861.

  • Positive control
    • IHC-P: Human tonsil tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A/G purified
  • Purification notes
    ab200699 is purified from bioreactor concentrate by Protein A/G.
  • Clonality
    Monoclonal
  • Clone number
    IG266
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab200699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Incubate with primary antibody for 30 minutes at RT.

WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 36 kDa.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.

Incubate with primary antibody for 30 minutes at RT.

ICC/IF Use a concentration of 0.5 - 1 µg/ml.
Flow Cyt Use 0.5-1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

IP Use 0.5-1 µg for 500 µg of chromatin.
ELISA Use at an assay dependent concentration.

Target

  • Relevance
    IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
  • Cellular localization
    Cell Membrane and Secreted
  • Database links
  • Alternative names
    • FLJ39988 antibody
    • FLJ40036 antibody
    • FLJ40253 antibody
    • FLJ40587 antibody
    • FLJ40789 antibody
    • FLJ40834 antibody
    • G1m marker antibody
    • G2m marker antibody
    • G3m marker antibody
    • G4m marker antibody
    • HDC antibody
    • Heavy chain disease protein antibody
    • Human immunglobulin G antibody
    • Ig gamma 1 chain C region antibody
    • Ig gamma 2 chain C region antibody
    • Ig gamma 3 chain C region antibody
    • Ig gamma 4 chain C region antibody
    • IgG heavy chain locus antibody
    • IGHG 1 antibody
    • IGHG 2 antibody
    • IGHG 3 antibody
    • IGHG 4 antibody
    • IGHG1 antibody
    • IGHG2 antibody
    • IGHG3 antibody
    • IGHG4 antibody
    • Immunoglobulin Gm1 antibody
    • Immunoglobulin Gm3 antibody
    • Immunoglobulin Gm4 antibody
    • immunoglobulin heavy constant gamma 1 (G1m marker) antibody
    • Immunoglobulin heavy constant gamma 1 antibody
    • immunoglobulin heavy constant gamma 2 (G2m marker) antibody
    • Immunoglobulin heavy constant gamma 2 antibody
    • immunoglobulin heavy constant gamma 3 (G3m marker) antibody
    • Immunoglobulin heavy constant gamma 3 antibody
    • immunoglobulin heavy constant gamma 4 (G4m marker) antibody
    • Immunoglobulin heavy constant gamma 4 antibody
    • MGC117419 antibody
    • MGC45809 antibody
    see all

Images

  • The wells were coated with ab200699 at 5 µg/ml at 50 µl/well overnight at 4°C, followed by a 5% BSA blocking step for 2 hours at RT. Human IgG ab98981 was then added starting at 100 µg/ml and plasma/serum at 1:50 and gradually diluted 1:4, 50 µl/well for 2 hours. ab201842 was then added at 1:10,000 dilution, 50 µl/well for 2 hours. HRP-streptavidin ab7403 was used at 1:10,000 dilution for 1 hour.

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for IgG with ab200699 at 1 µg/ml in immunohistochemical analysis.

References

This product has been referenced in:
  • Wang L  et al. Protein phosphatase 2A as a new target for downregulating osteoclastogenesis and alleviating titanium particle-induced bone resorption. Acta Biomater 73:488-499 (2018). Read more (PubMed: 29656074) »
  • Yin D  et al. Pro-Angiogenic Role of LncRNA HULC in Microvascular Endothelial Cells via Sequestrating miR-124. Cell Physiol Biochem 50:2188-2202 (2018). Read more (PubMed: 30415256) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization
No
Specification
Spleen
Blocking step
BSA as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Formaldehyde

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Verified customer

Submitted Mar 18 2019

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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