Anti-Human IgG antibody [IG266] (ab200699)
- Datasheet
- References (7)
- Protocols
Overview
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Product name
Anti-Human IgG antibody [IG266]
See all Human IgG primary antibodies -
Description
Mouse monoclonal [IG266] to Human IgG -
Host species
Mouse -
Specificity
ab200699 recognizes a protein of 75 kDa, identified as gamma heavy chain of Human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. -
Tested applications
Suitable for: IHC-P, WB, IHC-Fr, ICC/IF, Flow Cyt, IP, ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human IgG. P01857, P01859, P01860, P01861.
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Positive control
- IHC-P: Human tonsil tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
ab200699 is purified from bioreactor concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
IG266 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab200699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate with primary antibody for 30 minutes at RT. |
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| WB | Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 36 kDa. | |
| IHC-Fr | Use a concentration of 0.5 - 1 µg/ml. Incubate with primary antibody for 30 minutes at RT. |
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| ICC/IF | Use a concentration of 0.5 - 1 µg/ml. | |
| Flow Cyt | Use 0.5-1µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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| IP | Use 0.5-1 µg for 500 µg of chromatin. | |
| ELISA | Use at an assay dependent concentration. |
Target
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Relevance
IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). -
Cellular localization
Cell Membrane and Secreted -
Database links
- Entrez Gene: 3500 Human
- Entrez Gene: 3501 Human
- Entrez Gene: 3502 Human
- Entrez Gene: 3503 Human
- Omim: 147100 Human
- Omim: 147110 Human
- Omim: 147120 Human
- Omim: 147130 Human
see all -
Alternative names
- FLJ39988 antibody
- FLJ40036 antibody
- FLJ40253 antibody
see all
Images
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![ELISA - Anti-Human IgG antibody [IG266] (ab200699)](https://www.abcam.com/ps/products/200/ab200699/Images/ab200699-257618-anti-human-igg-antibody-ig266-elisa.jpg)
ELISA - Anti-Human IgG antibody [IG266] (ab200699)The wells were coated with ab200699 at 5 µg/ml at 50 µl/well overnight at 4°C, followed by a 5% BSA blocking step for 2 hours at RT. Human IgG ab98981 was then added starting at 100 µg/ml and plasma/serum at 1:50 and gradually diluted 1:4, 50 µl/well for 2 hours. ab201842 was then added at 1:10,000 dilution, 50 µl/well for 2 hours. HRP-streptavidin ab7403 was used at 1:10,000 dilution for 1 hour.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (ab200699)](https://www.abcam.com/ps/products/200/ab200699/Images/ab200699-241482-anti-human-igg-antibody-ig266-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (ab200699)Formalin-fixed, paraffin-embedded human tonsil tissue stained for IgG with ab200699 at 1 µg/ml in immunohistochemical analysis.
Protocols
References
This product has been referenced in:
- Stockdale L et al. HIV, HCMV and mycobacterial antibody levels: a cross-sectional study in a rural Ugandan cohort. Trop Med Int Health 24:247-257 (2019). Read more (PubMed: 30506614) »
- Lei JJ et al. Long noncoding RNA CDKN2B-AS1 interacts with transcription factor BCL11A to regulate progression of cerebral infarction through mediating MAP4K1 transcription. FASEB J 33:7037-7048 (2019). Read more (PubMed: 30870006) »
