Anti-Human IgG antibody [IG266] (ab200699)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-Human IgG antibody [IG266]
See all Human IgG primary antibodies -
DescriptionMouse monoclonal [IG266] to Human IgG
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Host speciesMouse
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Specificityab200699 recognizes a protein of 75 kDa, identified as gamma heavy chain of Human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes.
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Tested applicationsSuitable for: IHC-P, WB, IHC-Fr, ICC/IF, Flow Cyt, IP, ELISAmore details
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Species reactivityReacts with: Human
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Immunogen
Full length native protein (purified) corresponding to Human IgG. P01857, P01859, P01860, P01861.
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Positive control
- IHC-P: Human tonsil tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
PurityProtein A/G purified
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Purification notesab200699 is purified from bioreactor concentrate by Protein A/G.
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ClonalityMonoclonal
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Clone numberIG266
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IsotypeIgG2a
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Light chain typekappa
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab200699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate with primary antibody for 30 minutes at RT. |
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| WB | Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 36 kDa. | |
| IHC-Fr | Use a concentration of 0.5 - 1 µg/ml. Incubate with primary antibody for 30 minutes at RT. |
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| ICC/IF | Use a concentration of 0.5 - 1 µg/ml. | |
| Flow Cyt | Use 0.5-1µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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| IP | Use 0.5-1 µg for 500 µg of chromatin. | |
| ELISA | Use at an assay dependent concentration. |
Target
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RelevanceIgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
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Cellular localizationCell Membrane and Secreted
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Database links
- Entrez Gene: 3500 Human
- Entrez Gene: 3501 Human
- Entrez Gene: 3502 Human
- Entrez Gene: 3503 Human
- Omim: 147100 Human
- Omim: 147110 Human
- Omim: 147120 Human
- Omim: 147130 Human
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Alternative names
- FLJ39988 antibody
- FLJ40036 antibody
- FLJ40253 antibody
see all
Images
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![ELISA - Anti-Human IgG antibody [IG266] (ab200699)](https://www.abcam.com/ps/products/200/ab200699/Images/ab200699-257618-ab201842ELISA.jpg)
ELISA - Anti-Human IgG antibody [IG266] (ab200699)The wells were coated with ab200699 at 5 µg/ml at 50 µl/well overnight at 4°C, followed by a 5% BSA blocking step for 2 hours at RT. Human IgG ab98981 was then added starting at 100 µg/ml and plasma/serum at 1:50 and gradually diluted 1:4, 50 µl/well for 2 hours. ab201842 was then added at 1:10,000 dilution, 50 µl/well for 2 hours. HRP-streptavidin ab7403 was used at 1:10,000 dilution for 1 hour.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (ab200699)](https://www.abcam.com/ps/products/200/ab200699/Images/ab200699-241482-ab200699.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (ab200699)Formalin-fixed, paraffin-embedded human tonsil tissue stained for IgG with ab200699 at 1 µg/ml in immunohistochemical analysis.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Wang L et al. Protein phosphatase 2A as a new target for downregulating osteoclastogenesis and alleviating titanium particle-induced bone resorption. Acta Biomater 73:488-499 (2018). Read more (PubMed: 29656074) »
- Yin D et al. Pro-Angiogenic Role of LncRNA HULC in Microvascular Endothelial Cells via Sequestrating miR-124. Cell Physiol Biochem 50:2188-2202 (2018). Read more (PubMed: 30415256) »
