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    human-igg-antibody-ig266-ab200699.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Anti-Human IgG antibody [IG266] (ab200699)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (17)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (ab200699)
  • SDS-PAGE - Anti-Human IgG antibody [IG266] (ab200699)

Key features and details

  • Mouse monoclonal [IG266] to Human IgG
  • Suitable for: IHC-P, SDS-PAGE
  • Reacts with: Human
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-Human IgG antibody [IG266]
    See all Human IgG primary antibodies
  • Description

    Mouse monoclonal [IG266] to Human IgG
  • Host species

    Mouse
  • Specificity

    ab200699 recognizes a protein of 75 kDa, identified as gamma heavy chain of Human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes.
  • Tested applications

    Suitable for: IHC-P, SDS-PAGEmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human IgG. P01857, P01859, P01860, P01861.

  • Positive control

    • IHC-P: Human tonsil tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    ab200699 is purified from bioreactor concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    IG266
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG

Associated products

  • Alternative Versions

    • Anti-Human IgG antibody [IG266] - BSA and Azide free (ab233885)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Related Products

    • Biotin Anti-Human IgG antibody [EPR12700] (ab201842)
    • Streptavidin (HRP) (ab7403)
    • Native Human IgG protein (ab91102)
    • Native Human IgG protein (ab98981)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab200699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (2)
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Incubate with primary antibody for 30 minutes at RT.

Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes.

SDS-PAGE
Use at an assay dependent concentration.
Notes
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Incubate with primary antibody for 30 minutes at RT.

Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes.

SDS-PAGE
Use at an assay dependent concentration.

Target

  • Relevance

    IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
  • Cellular localization

    Cell Membrane and Secreted
  • Database links

    • Entrez Gene: 3500 Human
    • Entrez Gene: 3501 Human
    • Entrez Gene: 3502 Human
    • Entrez Gene: 3503 Human
    • Omim: 147100 Human
    • Omim: 147110 Human
    • Omim: 147120 Human
    • Omim: 147130 Human
    • SwissProt: P01857 Human
    • SwissProt: P01859 Human
    • SwissProt: P01860 Human
    • SwissProt: P01861 Human
    see all
  • Alternative names

    • FLJ39988 antibody
    • FLJ40036 antibody
    • FLJ40253 antibody
    • FLJ40587 antibody
    • FLJ40789 antibody
    • FLJ40834 antibody
    • G1m marker antibody
    • G2m marker antibody
    • G3m marker antibody
    • G4m marker antibody
    • HDC antibody
    • Heavy chain disease protein antibody
    • Human immunglobulin G antibody
    • Ig gamma 1 chain C region antibody
    • Ig gamma 2 chain C region antibody
    • Ig gamma 3 chain C region antibody
    • Ig gamma 4 chain C region antibody
    • IgG heavy chain locus antibody
    • IGHG 1 antibody
    • IGHG 2 antibody
    • IGHG 3 antibody
    • IGHG 4 antibody
    • IGHG1 antibody
    • IGHG2 antibody
    • IGHG3 antibody
    • IGHG4 antibody
    • Immunoglobulin Gm1 antibody
    • Immunoglobulin Gm3 antibody
    • Immunoglobulin Gm4 antibody
    • immunoglobulin heavy constant gamma 1 (G1m marker) antibody
    • Immunoglobulin heavy constant gamma 1 antibody
    • immunoglobulin heavy constant gamma 2 (G2m marker) antibody
    • Immunoglobulin heavy constant gamma 2 antibody
    • immunoglobulin heavy constant gamma 3 (G3m marker) antibody
    • Immunoglobulin heavy constant gamma 3 antibody
    • immunoglobulin heavy constant gamma 4 (G4m marker) antibody
    • Immunoglobulin heavy constant gamma 4 antibody
    • MGC117419 antibody
    • MGC45809 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (ab200699)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (ab200699)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for IgG with ab200699 at 1 µg/ml in immunohistochemical analysis.

  • SDS-PAGE - Anti-Human IgG antibody [IG266] (ab200699)
    SDS-PAGE - Anti-Human IgG antibody [IG266] (ab200699)

    Lane 1: Non-reduced ab200699

    Lane 2: Reduced ab200699

    SDS-PAGE analysis of ab200699.

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (17)

Publishing research using ab200699? Please let us know so that we can cite the reference in this datasheet.

ab200699 has been referenced in 17 publications.

  • Yuan M  et al. UNC5C-knockdown enhances the growth and metastasis of breast cancer cells by potentiating the integrin a6/ß4 signaling pathway. Int J Oncol 56:139-150 (2020). PubMed: 31789389
  • Deng Y  et al. Long non-coding RNA F11-AS1 inhibits HBV-related hepatocellular carcinoma progression by regulating NR1I3 via binding to microRNA-211-5p. J Cell Mol Med 24:1848-1865 (2020). PubMed: 31880390
  • Wang X  et al. Long non-coding RNA LINC00473/miR-195-5p promotes glioma progression via YAP1-TEAD1-Hippo signaling. Int J Oncol 56:508-521 (2020). PubMed: 31894297
  • Feng C  et al. Tumor-Suppressive MicroRNA-216b Binds to TPX2, Activating the p53 Signaling in Human Cutaneous Squamous Cell Carcinoma. Mol Ther Nucleic Acids 20:186-195 (2020). PubMed: 32169806
  • Gao L  et al. The RBP1-CKAP4 axis activates oncogenic autophagy and promotes cancer progression in oral squamous cell carcinoma. Cell Death Dis 11:488 (2020). PubMed: 32587255
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Human IgG antibody [IG266]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Bio SB ImmunoRetriever with citrate
Permeabilization
No
Specification
Placenta
Fixative
Formaldehyde
Read More

Marcelo Pelajo-Machado

Verified customer

Submitted Mar 26 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Human IgG antibody [IG266]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization
No
Specification
Spleen
Blocking step
BSA as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Mar 18 2019

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