Anti-Human IgG antibody [IG266] (ab200699)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (ab200699)](https://www.abcam.com/ps/products/200/ab200699/Images/ab200699-241482-anti-human-igg-antibody-ig266-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (ab200699)")
Key features and details
- Mouse monoclonal [IG266] to Human IgG
- Suitable for: IHC-P, SDS-PAGE
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Human IgG antibody [IG266]
See all Human IgG primary antibodies -
Description
Mouse monoclonal [IG266] to Human IgG -
Host species
Mouse -
Specificity
ab200699 recognizes a protein of 75 kDa, identified as gamma heavy chain of Human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. -
Tested applications
Suitable for: IHC-P, SDS-PAGEmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human IgG. P01857, P01859, P01860, P01861.
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Positive control
- IHC-P: Human tonsil tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
ab200699 is purified from bioreactor concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
IG266 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (2) |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Incubate with primary antibody for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes. |
| SDS-PAGE |
Use at an assay dependent concentration.
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| Notes |
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate with primary antibody for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes. |
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SDS-PAGE
Use at an assay dependent concentration. |
Target
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Relevance
IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). -
Cellular localization
Cell Membrane and Secreted -
Database links
- Entrez Gene: 3500 Human
- Entrez Gene: 3501 Human
- Entrez Gene: 3502 Human
- Entrez Gene: 3503 Human
- Omim: 147100 Human
- Omim: 147110 Human
- Omim: 147120 Human
- Omim: 147130 Human
see all -
Alternative names
- FLJ39988 antibody
- FLJ40036 antibody
- FLJ40253 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (ab200699)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for IgG with ab200699 at 1 µg/ml in immunohistochemical analysis.
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![SDS-PAGE - Anti-Human IgG antibody [IG266] (ab200699)](https://www.abcam.com/ps/products/200/ab200699/Images/ab200699-390905-anti-igg-mouse-monoclonal-antibody.jpg)
SDS-PAGE - Anti-Human IgG antibody [IG266] (ab200699)Lane 1: Non-reduced ab200699
Lane 2: Reduced ab200699
SDS-PAGE analysis of ab200699.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab200699 has been referenced in 17 publications.
- Yuan M et al. UNC5C-knockdown enhances the growth and metastasis of breast cancer cells by potentiating the integrin a6/ß4 signaling pathway. Int J Oncol 56:139-150 (2020). PubMed: 31789389
- Deng Y et al. Long non-coding RNA F11-AS1 inhibits HBV-related hepatocellular carcinoma progression by regulating NR1I3 via binding to microRNA-211-5p. J Cell Mol Med 24:1848-1865 (2020). PubMed: 31880390
- Wang X et al. Long non-coding RNA LINC00473/miR-195-5p promotes glioma progression via YAP1-TEAD1-Hippo signaling. Int J Oncol 56:508-521 (2020). PubMed: 31894297
- Feng C et al. Tumor-Suppressive MicroRNA-216b Binds to TPX2, Activating the p53 Signaling in Human Cutaneous Squamous Cell Carcinoma. Mol Ther Nucleic Acids 20:186-195 (2020). PubMed: 32169806
- Gao L et al. The RBP1-CKAP4 axis activates oncogenic autophagy and promotes cancer progression in oral squamous cell carcinoma. Cell Death Dis 11:488 (2020). PubMed: 32587255