Key features and details
- Mouse monoclonal [KT131] to Human IgG
- Suitable for: Sandwich ELISA, WB, ELISA
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-Human IgG antibody [KT131]
See all Human IgG primary antibodies
DescriptionMouse monoclonal [KT131] to Human IgG
Specificityab211339 is specific for IgG1, IgG2, IgG3, IgG4. It does not cross-react with Human IgA, IgE and IgM.
Tested applicationsSuitable for: Sandwich ELISA, WB, ELISAmore details
Species reactivityReacts with: Human
Full length protein corresponding to Human Human IgG.
- Purified immunoglobulins IgG1, IgG2, IgG3, IgG4, IgG.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab211339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 5 µg/ml.|
|WB||1/500 - 1/10000.|
RelevanceIgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
Cellular localizationCell Membrane and Secreted
- FLJ39988 antibody
- FLJ40036 antibody
- FLJ40253 antibody
All lanes : Anti-Human IgG antibody [KT131] (ab211339) at 1/500 dilution
Lane 1 : Purified immunoglobulin IgG1
Lane 2 : Purified immunoglobulin IgG2
Lane 3 : Purified immunoglobulin IgG3
Lane 4 : Purified immunoglobulin IgG4
Lane 5 : Purified immunoglobulin IgG
Lane 6 : Purified immunoglobulin IgA
Lane 7 : Purified immunoglobulin IgE
Lane 8 : Purified immunoglobulin IgM
Sandwich ELISA for normal Human serum samples using ab211339 coated plate and HRP conjugated antibody.
Sandwich ELISA for purified IgG using ab211339 coated plate and HRP conjugated antibody.
ELISA using plates coated with Human antibodies with various isotypes.
ab211339 has been referenced in 1 publication.
- Wang L & Ji S Inhibition of Ubc9-Induced CRMP2 SUMOylation Disrupts Glioblastoma Cell Proliferation. J Mol Neurosci 69:391-398 (2019). PubMed: 31267313