Recombinant Anti-Human IgG antibody [RM116] (ab195574)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM116] to Human IgG
- Suitable for: Sandwich ELISA, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Human IgG antibody [RM116]
See all Human IgG primary antibodies -
Description
Rabbit monoclonal [RM116] to Human IgG -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISA, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human IgG. Human IgG purified from Human plasma.
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Positive control
- Human tonsil tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab195574 was purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM116 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab195574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Sandwich ELISA |
Use a concentration of 1 µg/ml.
May be paired with ab193185. |
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IHC-P |
Use a concentration of 0.5 - 2 µg/ml.
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Notes |
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Sandwich ELISA
Use a concentration of 1 µg/ml. May be paired with ab193185. |
IHC-P
Use a concentration of 0.5 - 2 µg/ml. |
Target
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Relevance
IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). -
Cellular localization
Cell Membrane and Secreted -
Database links
- Entrez Gene: 3500 Human
- Entrez Gene: 3501 Human
- Entrez Gene: 3502 Human
- Entrez Gene: 3503 Human
- Omim: 147100 Human
- Omim: 147110 Human
- Omim: 147120 Human
- Omim: 147130 Human
see all -
Alternative names
- FLJ39988 antibody
- FLJ40036 antibody
- FLJ40253 antibody
see all
Images
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Immunohistochemical analysis of Human tonsil tissue labeling Human IgG with ab195574 at 2 µg/ml.
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Sandwich ELISA using ab195574 as the capture antibody (100 ng/well), and biotinylated anti-Human light chains ( κ+ λ) antibody as the detection antibody (1 ug/mL), followed by an alkaline phosphatase conjugated streptavidin.
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Sandwich ELISA using ab195574 as the capture antibody (100 ng/well), and biotinylated anti-Human light chains ( κ+ λ) antibody as the detection antibody (1 ug/mL), followed by an alkaline phosphatase conjugated streptavidin.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab195574 has been referenced in 1 publication.
- Li H et al. Epithelial-mesenchymal transition in chronic rhinosinusitis (CRS) and the prognostic value of a-SMA in postoperative outcomes of patients with CRS. Mol Med Rep 20:2441-2449 (2019). PubMed: 31322276