Overview

  • Product name

    Human IgG ELISA Kit
    See all IgG kits
  • Detection method

    Colorimetric
  • Sample type

    Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 20 pg/ml
  • Range

    0.02 ng/ml - 15 ng/ml
  • Recovery

    117 %

    Sample specific recovery
    Sample type Average % Range
    Serum 116.1 105% - 123%
    Plasma 118.1 99% - 126%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human IgG ELISA kit is designed for the quantitative measurement of Human IgG in serum and plasma. (UniprotID: Q60590)


     

  • Notes

    The Human immune system consists of two functional components classified as the innate system (the physical, biochemical and cellular barriers), and the adaptive immune system (including lymphocytes and immunoglobulins). Immunoglobulins are the key elements of the humoral immune response in vertebrate against parasitic invasion.

    The polypeptide chains of immunoglobulins composed of two identical heavy (H) chains and two identical light (L) chains linked together by inter-chain disulfide bonds. While the amino-terminal portions that exhibits highly variable amino-acid composition are involved in antigen binding, the C terminal constant parts are involved in complement binding, placental passage and binding to cell membranes. Based upon the variation of the constant region of the heavy chain, nine immunoglobulin heavy chain isotypes are found in humans: IgA (with subclasses IgA1 and IgA2), IgD, IgE, IgM, and IgG (with subclasses IgG1, IgG2, IgG3, and IgG4).

    IgG is the predominant immunoglobulin in the serum, which accounts for 75% of the total serum antibody of healthy individuals. IgG has a molecular weight of about 150 kDa. Four distinct subgroups of human IgG (IgG1, IgG2, IgG3, and IgG4) were first demonstrated in the 1960’s by using polyclonal antisera prepared in animals immunized with human myeloma proteins.

    The capture antibody of the kit ab100547 detects the Fc portion of the heavy chain of all subclasses of human IgG and the detection antibody detects the F(ab’)2 fragment of IgG.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab100547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100547

Protocols

References

This product has been referenced in:

  • Yamamoto M  et al. Analysis of allergic reaction in IgG4-related disease. Mod Rheumatol N/A:1-3 (2019). Read more (PubMed: 30676808) »
  • Ciupe SM  et al. A Bistable Switch in Virus Dynamics Can Explain the Differences in Disease Outcome Following SIV Infections in Rhesus Macaques. Front Microbiol 9:1216 (2018). Read more (PubMed: 29930544) »
See all 8 Publications for this product

Customer reviews and Q&As

1-10 of 14 Abreviews or Q&A

Seric IgG in healthy controls

Excellent Good 4/5 (Ease of Use)
Abreviews
We measured IgG in serum from 8 healthy controls. We performed three dilutions of each serum: 1/10exp7, 5*1/10exp7 and 1/10exp6. The best dilution in our samples was 1/10exp6.
Abcam recomend a dilution of 1/10exp7, but we strongly recommend (at least for healthy controls), firstly to test more than one dilution in order to work in a proper range (maybe 1/10exp7 and 1/10exp6).

Abcam user community

Verified customer

Submitted Jun 13 2019

assaying saliva samples with human IgG ELISA kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews
I measured IgG in saliva samples with the human IgG ELISA kit. Two saliva samples from healthy controls were used, each were diluted at 1:10000, 1:100000, 1:1000000, 1:10000000 with assay diluent.

Abcam user community

Verified customer

Submitted Apr 12 2019

IgG levels in human milk smaples by ELISA

Average Average 3/5 (Ease of Use)
Abreviews
Abcam´s IgG human ELISA kit was performed according to manufacturer protocol except to sample preparation, because this protocol was not previously validated for human milk samples. Therefore, we had to dilute for achieve the expected concentrations in samples. For example, we did a dilution of 1: 1,000,000 to get 1-3 mg/mL concentrations (data from scientific studies) at follows:
Add 1 μL of serum human milk into a tube with 99 μL 1X Assay Diluent B to prepare a 100-fold diluted sample. Mix through and then pipette 1 μL of prepared 100-fold diluted sample into a tube with 99 μL 1X Assay Diluent to prepare a 10,000 fold diluted sample. Mix through and then pipette 1 μL of prepared 10,000-fold diluted sample into a tube with 99 μL 1X Assay
More optimized dilutions are required to improve the sensibility of the low concentrations. Moreover, it’s important to point out that these immunoglobulin levels are low in milk samples, unlike serum from blood. It would be advisable optimize the type of diluent and standards to this type of sample. Despite of these reasons, IgG was detected in all samples, showing levels between 0,200 and 500 mg/mL.
In our opinion, it would be necessary positive controls to perform the analysis.

Abcam user community

Verified customer

Submitted Aug 20 2014

Answer

1. I can confirm that the standard included with this kit is total human IgG purified from plasma

2. This kit can detect all IgG subclasses, however we have not determined the relative sensitivity to each.

Read More

Answer

Epitope mapping has not been performed. However, the capture antibody is a mouse monoclonal and recognizes the Fc portion and the detection antibody is polyclonal and recognizes the Fab portion.

Read More

Answer

Sample binds to an antibody specific for Human IgG coated on the 96-well plate.

Read More

Answer

The standard of the kit is total human IgG purified from plasma. We do not sell kit components separately, however the equivalent in our catalogue would be ab98981, www.abcam.com/ab98981.

For the assay diluent I would recommend PBS buffer.

Read More

Answer

You are right with your calculations where at the end of the Item F preparation, you will have a 8,000uL volume. Since100uL is used for each well, you will have enough for rougly 80 wells. However, there will be some volume lost during pipetting,transfer, etc therefore each Item F vial has extra volume. Therefore, with 2 vials of Item F, you will have more than enough solution for 96 wells.

Read More

Question
Answer

For this kit, there is no need to use positive control. The kit has an anti-Human IgG antibody coated on the well, and you simply need to add your sample (unknown protein amount) together with a fixed concentration of standard protein (Item C).You can then determine how much protein you have in your sample by plotting the standard protein amount vs OD readout, and read your sample protein amount from the graph.

Read More

Answer

We unfortunately do not have bigger sizes for this kit. You can however order 2 kits at a time, if you are interested in more of these.

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1-10 of 14 Abreviews or Q&A

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