Recombinant

Recombinant Anti-Human IgG Lambda Light Chain antibody [RM127] (ab195573)

Overview

  • Product name

    Anti-Human IgG Lambda Light Chain antibody [RM127]
  • Description

    Rabbit monoclonal [RM127] to Human IgG Lambda Light Chain
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, ELISA, ICCmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Human IgG Lambda Light Chain. Human IgG purified from Human plasma.

  • Positive control

    • Human tonsil tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 48% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab195573 was purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RM127
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab195573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P Use a concentration of 0.5 - 2 µg/ml.
ELISA Use a concentration of 0.05 - 0.2 µg/ml.
ICC Use a concentration of 0.5 - 2 µg/ml.

Target

  • Relevance

    All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • Constant region of lambda light chains antibody
    • Ig lambda chain C regions antibody
    • IGLC antibody
    • Immunoglobulin lambda constant 1 antibody
    • Mcg marker antibody
    see all

Images

  • Immunohistochemical analysis of Human tonsil tissue labeling Human IgG Lambda Light Chain with ab195573 at 2 µg/ml.

References

ab195573 has not yet been referenced specifically in any publications.

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