Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM127] to Human IgG Lambda Light Chain
- Suitable for: Flow Cyt, IHC-P, ELISA, ICC
- Reacts with: Human
Product nameAnti-Human IgG Lambda Light Chain antibody [RM127]
DescriptionRabbit monoclonal [RM127] to Human IgG Lambda Light Chain
Tested applicationsSuitable for: Flow Cyt, IHC-P, ELISA, ICCmore details
Species reactivityReacts with: Human
Full length native protein (purified) corresponding to Human Human IgG Lambda Light Chain. Human IgG purified from Human plasma.
- Human tonsil tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
Purification notesab195573 was purified from an animal origin–free culture supernatant.
Our Abpromise guarantee covers the use of ab195573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||Use a concentration of 0.5 - 2 µg/ml.|
|ELISA||Use a concentration of 0.05 - 0.2 µg/ml.|
|ICC||Use a concentration of 0.5 - 2 µg/ml.|
RelevanceAll five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).
- Constant region of lambda light chains antibody
- Ig lambda chain C regions antibody
- IGLC antibody
ab195573 has not yet been referenced specifically in any publications.