Overview

  • Product name
    Human IgG1 ELISA Kit
    See all IgG1 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Saliva, Urine, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 0.05 ng/ml
  • Range
    0.05 ng/ml - 40 ng/ml
  • Recovery

    84 %

    Sample specific recovery
    Sample type Average % Range
    Serum 87 75% - 97%
    Plasma 81.5 71% - 94%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IgG1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IgG1 in serum and plasma.

    This assay employs an antibody specific for Human IgG1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IgG1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IgG1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IgG1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Sandwich ELISA - IgG1 Human ELISA Kit (ab100548)
    Sandwich ELISA - IgG1 Human ELISA Kit (ab100548)

    Human IgG1 measured in biological fluids showing quantity (pg) per mL of tested sample. Human serum and plasma were diluted 1e6 - 64e6 fold. Human saliva and urine were diluted 1e3-64e3 fold.

  • Typical standard curve
    Typical standard curve

    Representative standard curve using ab100548

Protocols

References

ab100548 has not yet been referenced specifically in any publications.

Publishing research using ab100548? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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13 reviews or Q&A

10
Votes: Highest First

Detection of Trastuzumab in mouse plasma samples

 Good Average 3/5 (Ease of Use)
Abreviews
The assay was tested for quantification of Trastuzumab plasma levels in a pharmacokinetic experiment in mice.
The standard curve was generated with both the recombinant IgG1 standard provided in the kit and the Trastuzumab, as comparison of relative O.D.
Each standard was assayed in duplicate, following the manufacturer instructions.
Plasma samples were diluted 10,000 fold with 1X Assay Diluent and gave the expected O.D. values.
abreview image
Username

Dr. Gianfranco Pasut

Verified customer

Submitted Apr 12 2017

I understand that the capture antibody for kit Ab100548 is mouse monoclonal. We would like to use this kit to detect levels of human IgG1 in rat samples. Will the kit work, or would you expect cross reaction of the mouse capture antibody with rat IgG? ...

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Hello! The laboratory is unsure if this would cross-react with rat or not, they have not tested it and have no data on this. Please let me know if you have any further questions.

What is the species that the polyclonal antibody is raised in?

Thank you for contacting us.

Regarding our Human IgG1 ELISA kit, the host species for the capture antibody is mouse and it is monoclonal; the detection antibody is goat, polyclonal. I have attached the manual for your reference.

I ...

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When doing the final analysis, do you have to take the correction factor into account?

Thank you for calling Abcam last week.

I have talked tothe lab and yes youwill need to multiplyyour sample concentration results by the sample dilution used in order to determine the actual levels of IgG1 in their samples. If it helps, the lev...

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Our customer is going to purchase ab100548 and would like to know if there is a cross-reactivity with rat IgG.

Cross-reactivity for ab100548 with rat has not been tested.
I can confirm that the capture antibody is monoclonal and the detection antibody is polyclonal. For the most part we do not see cross-species reactivity with sandwich based assays.

As promised I took a picture of the fungus growing in Item E. I received
the item E replacement from you and the new kit, thank you.
As well, I would like to thank you for the sweets, it was a nice thought.
In the future, I would suggest...

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Thank you for your email and the image.

I will send it to the supplying lab. Also, thank you for your suggestions, which I will pass on as well. I can assure you that this problem with the item E has been in discussion recently and we are loo...

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Thank you for the info.
I have absolutely used ddh2o for the reconstitution of the item E. However,
that should not even
be an issue because the fungus also grew in the item E stock solution
which was in the fridge. I am currently a...

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Thank you for your reply.

Yes, you are making a good point there - the fungus growing in the stock solution without adding any water. I will let the lab know and proceed with arranging the replacement for item E.

Enjoy your confer...

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ab100548: item E (assay diluent) has fungal contamination
after filtration obtained different results as before filtration of fungus
at the beginning: hazy solution, overtime, fungus grew bigger

lot GR65263-3 (Jan 2011) - used

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Thank you for your patience.

I have heard back from the supplying lab with the following information:

For ab100692: The plate is only coated with the capture antibody.

For ab100548: Assay Diluent B does contain some prote...

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ab100548: item E (assay diluent) has fungal contamination
after filtration obtained different results as before filtration of fungus
at the beginning: hazy solution, overtime, fungus grew bigger

lot GR65263-3 (Jan 2011) - used

Read More

Thank you for contacting us.

I am so sorry to hear about the problem you are having with our kits.

For ab100548, I have arranged for the replacement of 1 kit and am in touch with the lab to replace item E for the second kit.
<...

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Does this kit recognize the Fc or Fab fragment of the human IgG?

Thank you for contacting Abcam

The capture antibody in the ab100548 kit recognizes the Fc portion of IgG1. The detection antibody recognizes the Fab portion of IgG. When used as a pair, the kit detects IgG1.

Please let me know if th...

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1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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