Key features and details
- One-wash 90 minute protocol
- Sensitivity: 750 pg/ml
- Range: 1.56 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman IgG2 ELISA Kit
Intra-assay Sample n Mean SD CV% Serum 8 5.3% Inter-assay Sample n Mean SD CV% Serum 3 4.3%
Sample typeCell culture supernatant, Saliva, Milk, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma
Assay typeSandwich (quantitative)
Range1.56 ng/ml - 100 ng/ml
Sample specific recovery Sample type Average % Range Saliva 104 93% - 124% Milk 90 86% - 94% Urine 96.5 90% - 101% Serum 99.7 97.4% - 102.4% Hep Plasma 101.3 97.4% - 107.4% EDTA Plasma 102.5 98% - 105% Cit plasma 107.5 103% - 110%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Human
Does not react with: Goat, Cow, Pig
Human IgG2 ELISA Kit (ab202402) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IgG2 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, milk, saliva, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IgG2 with 750 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Immunoglobulin G (IgG) is a glycoprotein molecule which belongs to the immunoglobulin family of proteins known as antibodies. Immunoglobulins are the key component of humoral immunity. IgG has an approximate molecular weight of about 150kDa and it is composed of four peptide chains: two identical heavy chains (γ) of about 50kDa and two identical light chains (κ) of about 25kDa each. The heavy chains are linked to each other and to the light chain by disulfide bonds. At the N terminus, both the heavy and the light chain contain variable regions (VH and VL) which account for antibody diversity. At the C terminus, both chains contain constant regions (CH and CL) but only CH mediates effector functions. Structurally the IgG molecule may be divided into: (1) the Fragment antigen binding region (Fab) containing the VL, VH, CL and CH2 all of which shape the antigen binding site and (2) the Fragment crystallizable region (Fc) containing CH domains 2 – 4 which stabilize the antibody and bind to the Fc receptor on the surface of macrophages, neutrophils, natural killer cells as well as to complement proteins to mediate therefore physiological effects.
IgG is synthesized and secreted by plasma B cells in response to an immunogen after recognition of specific epitopes on the antigen and it is generated following class switching and maturation of an antibody response, thus providing immune protection. There are four subclasses of IgG in humans (IgG 1, 2, 3, 4) with variable affinity to Fc receptors and complement. The levels of IgG are generally considered to be indicative of an individual’s immune status and are found decrease in conditions such as hypogammaglobulinemia and X-linked agammaglobulinemia. IgG accounts for 75% of the total human protein and can be found in serum, lymphatic fluid, cerebrospinal fluid, colostrum, milk, urine, saliva, tissues, sweat and skin sebum.
IgG2, the second largest part of IgG isotypes in humans, comprises 20-25% of the main subclass and is the prevalent immune response against carbohydrate-/polysaccharide antigens. Among all IgG isotypes, a deficiency in IgG2 is the most common one and associated with recurring airway/respiratory infections caused by encapsulated bacteria such as pneumococci and/or Haemophilus influenza type B.
PlatformMicroplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Human IgG2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human IgG2 Capture Antibody (Lyophilized) 1 vial Human IgG2 Lyophilized Purified Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Background-subtracted data values (mean +/- SD) are graphed.
Native human IgG2 protein was measured in citrate plasma (1:300,000), EDTA plasma (1:200,000), and heparin plasma (1:300,000) in a 2 fold dilution series in Sample Diluent NS. The interpolated dilution factor corrected values are graphed (mean +/- SD).
Native human IgG2 protein was measured in milk (1:800), urine (1:30), and saliva (1:400) in a 2-fold dilution series in Sample Diluent NS. The interpolated dilution factor corrected values are graphed (mean +/- SD).
Ten individual healthy male donors were evaluated for the presence of IgG2 in serum using this assay. Results were interpolated from the standard curve in Sample Diluent NS and corrected by sample dilution (1:500,000). In the 10 individual donors, the mean level of IgG2 is 5.6 mg/mL with a range of 2.5 – 9.1 mg/mL and a standard deviation of 2.16 mg/mL.
ab202402 has not yet been referenced specifically in any publications.