Key features and details
- Mouse monoclonal [5G12] to Human IgG3
- Suitable for: ELISA
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Human IgG3 antibody [5G12]
See all Human IgG3 primary antibodies
DescriptionMouse monoclonal [5G12] to Human IgG3
SpecificitySubclass-specific for human IgG3. No allotype restriction. Hinge-region specific.
Tested applicationsSuitable for: ELISAmore details
Species reactivityReacts with: Human
IgG3 from human serum.
General notesConcentration varies from lot to lot and can be provided on request.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein A purified
Purification notesChromatography on protein A Sepharose
Our Abpromise guarantee covers the use of ab1928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
Specific antibody titer is 1/300,000 by indirect ELISA.
RelevanceIgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab1928 has been referenced in 3 publications.
- Nair M et al. Dexamethasone-Mediated Upregulation of Calreticulin Inhibits Primary Human Glioblastoma Dispersal Ex Vivo. Int J Mol Sci 19:N/A (2018). PubMed: 29443896
- Poitelon Y et al. Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination. Nat Commun 6:8303 (2015). WB ; Rat . PubMed: 26383514
- Medeiros MM et al. Natural antibody response to Plasmodium falciparum merozoite antigens MSP5, MSP9 and EBA175 is associated to clinical protection in the Brazilian Amazon. BMC Infect Dis 13:608 (2013). ELISA ; Human . PubMed: 24373342