Overview

  • Product name

    Anti-Human IgG3 antibody [RM119]
    See all Human IgG3 primary antibodies
  • Description

    Rabbit monoclonal [RM119] to Human IgG3
  • Host species

    Rabbit
  • Specificity

    Reacts to the heavy chain of human IgG3. No cross reactivity with human IgG1, IgG2, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
  • Tested applications

    Suitable for: Sandwich ELISA, ICC/IF, Flow Cyt, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human Human IgG3. Purified Native Human IgG3 from Human Plasma
    Database link: P01860

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 48% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Protein A affinity purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RM119
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab193172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Use: 50ng/well – 200ng/well (for Capture);
0.05μg/mL – 0.2μg/mL (for Detection);

ICC/IF Use a concentration of 0.5 - 2 µg/ml.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P Use a concentration of 5 µg/ml.

.

ELISA Use at an assay dependent concentration.

Target

  • Relevance

    IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • DKFZp686H11213 antibody
    • FLJ39988 antibody
    • FLJ40036 antibody
    • FLJ40253 antibody
    • FLJ40587 antibody
    • FLJ40789 antibody
    • FLJ40834 antibody
    • G3m marker antibody
    • HDC antibody
    • Heavy chain disease protein antibody
    • Ig gamma 3 chain C region antibody
    • IgG3 antibody
    • IGHG 3 antibody
    • IGHG3 antibody
    • IGHG3 protein antibody
    • Immunoglobulin gamma 3 (Gm marker) antibody
    • Immunoglobulin gamma 3 antibody
    • Immunoglobulin heavy constant gamma 3 (G3m marker) antibody
    • Immunoglobulin heavy constant gamma 3 antibody
    • MGC45809 antibody
    see all

Images

  • A titer ELISA using ab193172. The plate was coated with different amounts of human IgG3. A serial dilution of ab193172 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • ELISA of human immunoglobulins shows ab193172 only reacted to human IgG3. No cross reactivity with Human IgG1, IgG2, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab193172 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • Sandwich ELISA using ab193172 as the capture antibody (100ng/well), and a biotinylated anti human light chains ( κ+ λ) antibody as the detection antibody, followed by an HRP conjugated streptavidin.

  • Sandwich ELISA using ab193172 as the capture antibody (100ng/well), and a biotinylated anti human light chains ( κ+ λ) antibody as the detection antibody, followed by an HRP conjugated streptavidin.

References

This product has been referenced in:

  • Hao Y  et al. Analysis of the relationship between Oxford classification, IgM deposition and multiple indexes and the adverse prognosis of patients with primary IgA nephropathy and related risk factors. Exp Ther Med 17:1234-1239 (2019). Read more (PubMed: 30679997) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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