Key features and details
- One-wash 90 minute protocol
- Sensitivity: 20 pg/ml
- Range: 93.75 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman IgG3 ELISA Kit
See all IgG3 kits
Intra-assay Sample n Mean SD CV% Human Serum 8 3.73% Inter-assay Sample n Mean SD CV% Human Serum 3 4.01%
Sample typeCell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma
Assay typeSandwich (quantitative)
Range93.75 pg/ml - 6000 pg/ml
Sample specific recovery Sample type Average % Range Serum 93 86% - 102% Cell culture media 95 92% - 99% Hep Plasma 84 81% - 89% EDTA Plasma 91 87% - 98% Cit plasma 91 81% - 102%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Human
Does not react with: Sheep, Goat, Cow, Pig
Human IgG3 ELISA Kit (ab201284) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IgG3 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IgG3 with 20 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Immunoglubulin G (IgG) is a glycoprotein molecule which belongs to the immunoglobulin family of proteins known as antibodies. Immunoglobulins are the key component of humoral immunity. IgG has an approximate molecular weight of about 150kDa and it is composed of four peptide chains: two identical heavy chains (γ) of about 50kDa and two identical light chains (κ) of about 25kDa each. The heavy chains are linked to each other and to the light chain by disulfide bonds. At the N terminus, both the heavy and the light chain contain variable regions (VH and VL) which account for antibody diversity. At the C terminus, both chains contain constant regions (CH and CL) but only CH mediates effector functions. Structurally the IgG molecule may be divided into: (1) the Fragment antigen binding region (Fab) containing the VL, VH, CL and CH2 all of which shape the antigen binding site and (2) the Fragment crystallizable region (Fc) containing CH domains 2 – 4 which stabilize the antibody and bind to the Fc receptor on the surface of macrophages, neutrophils, natural killer cells as well as to complement proteins to mediate therefore physiological effects.
IgG is synthesized and secreted by plasma B cells in response to an immunogen after recognition of specific epitopes on the antigen and it is generated following class switching and maturation of an antibody response, thus providing immune protection. There are four subclasses of IgG in Humans (IgG 1, 2, 3, 4) with variable affinity to Fc receptors and complement. It has been shown that during HIV-1 infection, antibodies with the same epitope specificity but of different subclass have different functions.
IgG3 comprises around 5 - 10% of total IgG and plays a major role in the immune responses against protein or polypeptide antigens. The affinity of the Fc region of IgG3 binds with very high affinity and mediates a strong antibody-dependent cellular cytotoxicity and complement dependent cytotoxicity. IgG3 does not bind to Protein A, but it does bind to Protein G and L. Antigen-specific IgG3 immune response has been associated with long-term protection from the Plasmodium falciparum parasite and Chikungunya virus and it has been shown to be correlated with a decreased HIV infection risk after vaccination induced immediate humoral response.
IgG3 deficiency is associated with Wiscott-Aldrich disease, systemic lupus erythematosus, juvenile diabetes mellitus, and peridontal infections. IgG3 deficiency has been associated with a history of recurrent infections, leading to chronic lung disease
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Human IgG3 Capture Antibody 1 x 600µl 10X Human IgG3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human IgG3 Lyophilized Purified Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
RelevanceIgG3, like IgG1, activates complement and has an affinity for fc receptors; thus IgG1 and IgG3 are the more effective of the IgG subclasses. An IgG3 deficiency is associated with Wiscott-Aldrich disease, systemic lupus erythematosus, juvenile diabetes mellitus, and peridontal infections. IgG anti-viral antibodies are restricted to IgG subclasses 1 and 3, with IgG3 antibodies being the first to appear in response to infection.
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Background-subtracted data values (mean +/- SD) are graphed.
Native human IgG3 protein was measured in serum diluted 1:150,000 in sample diluent NS prior to a 2-fold dilution series in Sample Diluent NS. Native Human IgG3 protein was measured in citrate plasma (1:125,000), EDTA plasma (1:150,000), and heparin plasma (1:200,000) in a 2-fold dilution series in Sample Diluent NS. The interpolated dilution factor corrected values are graphed (mean +/- SD).
Ten individual healthy male donors and one pooled sample (n = 50) were evaluated for the presence of IgG3 in serum using this assay. Results were interpolated from the standard curve in Sample Diluent NS and corrected by sample dilution (1:200,000). In the 10 individual donors, the mean level of IgG3 is 381.44 µg/mL with a range of 173.71 – 746.69 µg/mL and a standard deviation of 159.71 µg/mL.
ab201284 has not yet been referenced specifically in any publications.