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  1. Link

    human-igg4-pfcrsquo-hp6023-biotin-ab99818.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Anti-Human IgG4 pFc’ [HP6023] (Biotin) (ab99818)

  • Datasheet
  • SDS
Submit a review Submit a question References (1)

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  • ELISA - Anti-Human IgG4 pFc’ [HP6023] (Biotin) (ab99818)
  • Datasheet
  • References (1)
  • Protocols

Overview

  • Product name

    Anti-Human IgG4 pFc’ [HP6023] (Biotin)
    See all IgG4 secondary antibodies
  • Host species

    Mouse
  • Target species

    Human
  • Specificity

    ab99818 reacts with the pFc’ portion of the heavy chain of Human IgG4.
  • Tested applications

    Suitable for: ELISA, Dot blot, ICC/IF, IHC-Fr, WBmore details
  • Immunogen

    Other Immunogen Type corresponding to Human IgG4. Myeloma protein

  • Conjugation

    Biotin

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    HP6023
  • Isotype

    IgG3
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Human
    • IgG4
    • Biotin

Associated products

  • Alternative Versions

    • Anti-Human IgG4 pFc’ [HP6023] (ab99814)
    • Anti-Human IgG4 pFc’ [HP6023] (FITC) (ab99815)
    • Anti-Human IgG4 pFc’ [HP6023] (Alkaline Phosphatase) (ab99816)
    • Mouse Anti-Human IgG4 pFc’ [HP6023] (HRP) (ab99817)
    • Anti-Human IgG4 pFc’ [HP6023] (Phycoerythrin) (ab99819)

Applications

Our Abpromise guarantee covers the use of ab99818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000 - 1/2000.
Dot blot Use at an assay dependent dilution. for Dot and slot immunoblotting.
ICC/IF Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

Images

  • ELISA - Anti-Human IgG4 pFc’ [HP6023] (Biotin) (ab99818)
    ELISA - Anti-Human IgG4 pFc’ [HP6023] (Biotin) (ab99818)

    ELISA - Anti-Human IgG4 pFc [HP6023] (Biotin) (ab99818)

    ELISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4.

    Immunoglobulins were detected with serially diluted ab99818 followed by Streptavidin-HRP.

Datasheets and documents

    • Datasheet
    • SDS
  • References

    This product has been referenced in:

    • Boylan B & Miller CH Effects of pre-analytical heat treatment in factor VIII (FVIII) inhibitor assays on FVIII antibody levels. Haemophilia 24:487-491 (2018). Read more (PubMed: 29461004) »
    See 1 Publication for this product

    Publishing research using ab99818? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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