Anti-Human IgG4 pFc’ [HP6023] (Biotin) (ab99818)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-Human IgG4 pFc’ [HP6023] (Biotin)
See all IgG4 secondary antibodies -
Host speciesMouse
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Target speciesHuman
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Specificityab99818 reacts with the pFc’ portion of the heavy chain of Human IgG4.
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Tested applicationsSuitable for: ELISA, Dot blot, ICC/IF, IHC-Fr, WBmore details
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Immunogen
Other Immunogen Type corresponding to IgG4.
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ConjugationBiotin
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberHP6023
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IsotypeIgG3
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Light chain typekappa
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Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab99818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/1000 - 1/2000. | |
| Dot blot | Use at an assay dependent dilution. for Dot and slot immunoblotting. | |
| ICC/IF | Use at an assay dependent dilution. | |
| IHC-Fr | Use at an assay dependent dilution. | |
| WB | Use at an assay dependent dilution. |
Images
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![ELISA - Anti-Human IgG4 pFc’ [HP6023] (Biotin) (ab99818)](https://www.abcam.com/ps/products/99/ab99818/Images/ab99818-293781-ab99818ELISA.jpg)
ELISA - Anti-Human IgG4 pFc’ [HP6023] (Biotin) (ab99818)ELISA - Anti-Human IgG4 pFc [HP6023] (Biotin) (ab99818)
ELISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4.
Immunoglobulins were detected with serially diluted ab99818 followed by Streptavidin-HRP.
References
ab99818 has not yet been referenced specifically in any publications.
