Key features and details
- Chicken polyclonal to Human IgM
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgY
Product nameAnti-Human IgM antibody
See all Human IgM primary antibodies
DescriptionChicken polyclonal to Human IgM
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Full length native protein (purified) (Human): IgM from plasma.
- Human plasma protein
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein L purified
Purification notesab26867 is an affinity purified IgY.
Our Abpromise guarantee covers the use of ab26867 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).|
RelevanceIgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.
Cellular localizationCell Membrane and Secreted
- Immunoglobin heavy chain constant region mu antibody
- AGM1 antibody
- Constant region of heavy chain of IgM antibody
Anti-Human IgM antibody (ab26867) at 1/500 dilution + Human plasma protein
HRP conjugated goat anti-IgY at 1/1000 dilution
Predicted band size: 75 kDa
Observed band size: 25,75 kDa why is the actual band size different from the predicted?
Colorimetric method for signal development
ab26867 has not yet been referenced specifically in any publications.