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RecombinantRabMAb

Recombinant Anti-Human IgM antibody [EPR20731] (ab212201)

Submit a review Submit a question References (3)
Immunocytochemistry/ Immunofluorescence - Anti-Human IgM antibody [EPR20731] (ab212201)
  • Western blot - Anti-Human IgM antibody [EPR20731] (ab212201)
  • Flow Cytometry - Anti-Human IgM antibody [EPR20731] (ab212201)
  • Immunoprecipitation - Anti-Human IgM antibody [EPR20731] (ab212201)
  • Anti-Human IgM antibody [EPR20731] (ab212201)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20731] to Human IgM
  • Suitable for: WB, IP, Flow Cyt, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Human IgM antibody [EPR20731]
    See all Human IgM primary antibodies

  • Description

    Rabbit monoclonal [EPR20731] to Human IgM

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Raji whole cell lysate; human tonsil and fetal spleen tissue lysates. IP: Raji whole cell lysate. ICC/IF: Raji cells. Flow cytometry: Human PBMC cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20731

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab212201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 49 kDa.
IP
1/30.
Flow Cyt
1/50.
ICC/IF
1/100.
Notes
WB
1/1000. Predicted molecular weight: 49 kDa.
IP
1/30.
Flow Cyt
1/50.
ICC/IF
1/100.

Target

  • Relevance

    IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.

  • Cellular localization

    Cell Membrane and Secreted

  • Database links

    • Alternative names

      • Immunoglobin heavy chain constant region mu antibody
      • AGM1 antibody
      • Constant region of heavy chain of IgM antibody
      • DKFZp686I15196 antibody
      • DKFZp686I15212 antibody
      • FLJ00385 antibody
      • Ig mu chain C region antibody
      • IGHM antibody
      • IgM heavy chain constant region antibody
      • Immunoglobin heavy constant mu antibody
      • Immunoglobulin mu antibody
      • MGC104996 antibody
      • MGC52291 antibody
      • MU antibody
      • VH antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Human IgM antibody [EPR20731] (ab212201)
      Immunocytochemistry/ Immunofluorescence - Anti-Human IgM antibody [EPR20731] (ab212201)

      Immunofluorescent analysis of 100% methanol-fixed Raji (human Burkitt's lymphoma cell line) cells labeling IgM with ab212201 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in Raji cells.

      The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Western blot - Anti-Human IgM antibody [EPR20731] (ab212201)
      Western blot - Anti-Human IgM antibody [EPR20731] (ab212201)
      All lanes : Anti-Human IgM antibody [EPR20731] (ab212201) at 1/1000 dilution

      Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell lysate at 20 µg
      Lane 2 : Human tonsil tissue lysate at 20 µg
      Lane 3 : Human fetal spleen tissue lysate at 10 µg

      Secondary
      All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

      Developed using the ECL technique.

      Predicted band size: 49 kDa
      Observed band size: 78 kDa why is the actual band size different from the predicted?



      The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
      The molecular mass observed is consistent with the literature (PMID 16199094; PMID: 15955802; PMID 2107027).

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure times.

      Lanes 1 & 3: 3 minutes.

      Lane 2: 10 seconds.

    • Flow Cytometry - Anti-Human IgM antibody [EPR20731] (ab212201)
      Flow Cytometry - Anti-Human IgM antibody [EPR20731] (ab212201)

      Flow cytometric analysis of human PBMC (peripheral blood mononuclear cells) cell line labeling IgM with ab212201 at 1/50 (right panel) compared with an isotype control details (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody. 

      Human PBMCs co-stained with anti-CD19 (Y-axis); only CD19+ population give a positive signal for IgM. Gated on total viable cells.

    • Immunoprecipitation - Anti-Human IgM antibody [EPR20731] (ab212201)
      Immunoprecipitation - Anti-Human IgM antibody [EPR20731] (ab212201)

      IgM was immunoprecipitated from 0.35 mg of Raji (human Burkitt's lymphoma cell line) whole cell lysate with ab212201 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab212201 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

      Lane 1: Raji whole cell lysate 10 μg (Input).

      Lane 2: ab212201 IP in Raji whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab212201 in Raji whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 10 seconds.

    • Anti-Human IgM antibody [EPR20731] (ab212201)
      Anti-Human IgM antibody [EPR20731] (ab212201)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (3)

    Publishing research using ab212201? Please let us know so that we can cite the reference in this datasheet.

    ab212201 has been referenced in 3 publications.

    • Tan L  et al. Mesangial IgM deposition predicts renal outcome in patients with IgA nephropathy: a multicenter, observational study. Clin Exp Med N/A:N/A (2021). PubMed: 33837881
    • Hao Y  et al. Analysis of the relationship between Oxford classification, IgM deposition and multiple indexes and the adverse prognosis of patients with primary IgA nephropathy and related risk factors. Exp Ther Med 17:1234-1239 (2019). PubMed: 30679997
    • Fetz AE  et al. Surface Area to Volume Ratio of Electrospun Polydioxanone Templates Regulates the Adsorption of Soluble Proteins from Human Serum. Bioengineering (Basel) 6:N/A (2019). PubMed: 31480458

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