Recombinant
RabMAb

Recombinant Anti-Human IgM antibody [EPR20731] - BSA and Azide free (ab228524)

Overview

  • Product name

    Anti-Human IgM antibody [EPR20731] - BSA and Azide free
    See all Human IgM primary antibodies
  • Description

    Rabbit monoclonal [EPR20731] to Human IgM - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P01871

  • Positive control

    • ICC/IF: Raji cells.
  • General notes

    Ab228524 is the carrier-free version of ab212201. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab228524 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20731
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Relevance

    IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.
  • Cellular localization

    Cell Membrane and Secreted
  • Database links

  • Alternative names

    • Immunoglobin heavy chain constant region mu antibody
    • AGM1 antibody
    • Constant region of heavy chain of IgM antibody
    • DKFZp686I15196 antibody
    • DKFZp686I15212 antibody
    • FLJ00385 antibody
    • Ig mu chain C region antibody
    • IGHM antibody
    • IgM heavy chain constant region antibody
    • Immunoglobin heavy constant mu antibody
    • Immunoglobulin mu antibody
    • MGC104996 antibody
    • MGC52291 antibody
    • MU antibody
    • VH antibody
    see all

Images

  • IgM was immunoprecipitated from 0.35 mg of Raji (human Burkitt's lymphoma cell line) whole cell lysate with ab212201 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab212201 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Raji whole cell lysate 10 μg (Input).

    Lane 2: ab212201 IP in Raji whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab212201 in Raji whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212201).

  • Flow cytometric analysis of human PBMC (peripheral blood mononuclear cells) cell line labeling IgM with ab212201 at 1/50 (right panel) compared with an isotype control details (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody. 

    Human PBMCs co-stained with anti-CD19 (Y-axis); only CD19+ population give a positive signal for IgM. Gated on total viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212201).

  • Immunofluorescent analysis of 100% methanol-fixed Raji (human Burkitt's lymphoma cell line) cells labeling IgM with ab212201 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in Raji cells.

    The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    -ve control: PBS instead of ab212201, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212201).

References

ab228524 has not yet been referenced specifically in any publications.

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