Product nameAnti-Human IgM antibody [EPR5539-65-4]
See all Human IgM primary antibodies
DescriptionRabbit monoclonal [EPR5539-65-4] to Human IgM
Specificityab134159 only recognizes the mu chain of IgM.
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Unsuitable for: ICC
Species reactivityReacts with: Human
Full length native protein (purified) corresponding to Human Human IgM.
- Human plasma, tonsil and spleen lysates; Human tonsil tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 75 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceIgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.
Cellular localizationCell Membrane and Secreted
- Immunoglobin heavy chain constant region mu antibody
- AGM1 antibody
- Constant region of heavy chain of IgM antibody
All lanes : Anti-Human IgM antibody [EPR5539-65-4] (ab134159) at 1/1000 dilution
Lane 1 : Human plasma lysate
Lane 2 : Human tonsil lysate
Lane 3 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Observed band size: 75 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling Human IgM with ab134159 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.