Recombinant
RabMAb

Recombinant Anti-Human IgM antibody [EPR5539-65-4] (ab134159)

Overview

  • Product name

    Anti-Human IgM antibody [EPR5539-65-4]
    See all Human IgM primary antibodies
  • Description

    Rabbit monoclonal [EPR5539-65-4] to Human IgM
  • Host species

    Rabbit
  • Specificity

    ab134159 only recognizes the mu chain of IgM.
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: ICC
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Human IgM.

  • Positive control

    • Human plasma, tonsil and spleen lysates; Human tonsil tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5539-65-4
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 75 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Relevance

      IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.
    • Cellular localization

      Cell Membrane and Secreted
    • Database links

    • Alternative names

      • Immunoglobin heavy chain constant region mu antibody
      • AGM1 antibody
      • Constant region of heavy chain of IgM antibody
      • DKFZp686I15196 antibody
      • DKFZp686I15212 antibody
      • FLJ00385 antibody
      • Ig mu chain C region antibody
      • IGHM antibody
      • IgM heavy chain constant region antibody
      • Immunoglobin heavy constant mu antibody
      • Immunoglobulin mu antibody
      • MGC104996 antibody
      • MGC52291 antibody
      • MU antibody
      • VH antibody
      see all

    Images

    • All lanes : Anti-Human IgM antibody [EPR5539-65-4] (ab134159) at 1/1000 dilution

      Lane 1 : Human plasma lysate
      Lane 2 : Human tonsil lysate
      Lane 3 : Human spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Observed band size: 75 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling Human IgM with ab134159 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Sánchez-Rodríguez C  et al. Immunomodulatory effect of Polypodium leucotomos (Anapsos) in child palatine tonsil model. Int J Pediatr Otorhinolaryngol 107:56-61 (2018). Read more (PubMed: 29501312) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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